RESUMEN
Bacterial adhesion is an important step in tissue colonization and depends extensively on the surface properties of a bacterial cell. For many microorganisms the prerequisite for host body occupancy is a break in tissue continuity. The next step is ongoing tissue destruction by products of bacterial metabolism: microbial enzymes and toxins. This happens, for example, in the initial phase of periodontitis. The mechanisms of adhesion are related to the specific structures present on the bacterial cell surface. This article summarizes recent data about bacterial attachment to host cells.
Asunto(s)
Adhesión Bacteriana/fisiología , Infecciones Bacterianas/veterinaria , Animales , Infecciones Bacterianas/microbiología , Enfermedades Periodontales/microbiología , Enfermedades Periodontales/veterinariaRESUMEN
Lymphoid cell infiltration of periodontal tissues undergoing pathological destruction suggests a functional interdependence between connective tissue proteins such as collagen, fibronectin and elastin and mononuclear cells, mainly T lymphocytes. The aim of the study was to assess T lymphocyte proliferation after stimulation by mAb OKT3 and then costimulation by ECM (extracellular matrix) proteins, as well as T-cell adhesion to connective tissue proteins in patients with adult periodontitis, early-onset periodontitis, chronic gingivitis and experimental gingivitis. Controls included patients with clinically healthy periodontium. Statistical analysis of results showed a significantly decreased level of T lymphocyte proliferation in response to costimulating action of ECM proteins among patients with adult periodontitis as compared to the control group. Reactivity of T-cells was much higher in experimental gingivitis than in adult periodontitis, and significantly lower in chronic gingivitis than in controls. In addition, T-cell interaction with ECM proteins was abnormal in early-onset periodontitis patients, but was not statistically significant.
Asunto(s)
Proteínas de la Matriz Extracelular/inmunología , Gingivitis/inmunología , Periodontitis/inmunología , Linfocitos T/inmunología , Adulto , Anciano , Anticuerpos Monoclonales/inmunología , Adhesión Celular , Femenino , Humanos , Activación de Linfocitos , Masculino , Persona de Mediana Edad , Mitógenos/farmacologíaRESUMEN
The aim of study was the evaluation of periodontal pockets microflora in patients with advanced periodontitis. From each subject 16-20 samples were taken using paper points. Pooled sample after 60 s. mixing was serially diluted in reduced BHI. For total cell counts and for the isolation of black pigmented anaerobes Brucella agar supplemented with 5% sheep blood, hemin, menadione, with and without Kanamycin-Vancomycin mixture and BM agar plates were used. For isolation of A. actinomycetemcomitans TSBV agar plates were used. Cultures were incubated in anaerobic chamber at 37 degrees C for 7 days and TSBV agar plates in an atmosphere of 95% air-5% CO2 at 37 degrees C for 5 days. Microorganisms were identified by Gram staining, colony morphology, fluorescence in UV-light, haemagglutination of 3% sheep erythrocytes, fermentation of sugars, production of indole, urease (API 20A), specific enzymes (Rapid ID 32A). Twenty seven subjects with clinically recognized periodontitis were examined. Microorganisms important in periodontitis were isolated from periodontal pockets of almost all examined subjects. The number of bacteria obtained from the sample of one patient ranged from 1 x 10(4) CFU/ml to 3,6 x 10(6) CFU/ml. Porphyromonas gingivalis was identified in the samples taken from 17 patients, Prevotella intermedia-19, Actinobacillus actinomycetemcomitans -11, Fusobacterium nucleatum-9, Peptostreptococcus spp.-22.
Asunto(s)
Bacterias Aerobias/aislamiento & purificación , Bacterias Anaerobias/aislamiento & purificación , Bolsa Periodontal/microbiología , Adulto , Agar , Medios de Cultivo , Humanos , Persona de Mediana Edad , Especificidad de la EspecieAsunto(s)
Necesidades y Demandas de Servicios de Salud , Investigación sobre Servicios de Salud , Enfermedades Periodontales/epidemiología , Adulto , Cálculos Dentales/epidemiología , Femenino , Humanos , Masculino , Persona de Mediana Edad , Servicios de Salud del Trabajador , Enfermedades Periodontales/terapia , Índice Periodontal , Polonia/epidemiología , Población UrbanaRESUMEN
The position of the connective tissue attachment was determined by Ramfjord's method in subjects aged 20-29 and 40-49 years. In the determination four surfaces of all remaining incisors, canines, and premolars were considered. A defect of the connective tissue attachment was found in about 95% of the analysed dental surfaces in subjects aged 40-49 years. The frequency of occurrence of these defects varied depending on the type dental surface.
Asunto(s)
Enfermedades Periodontales/patología , Adulto , Tejido Conectivo/patología , Humanos , Persona de Mediana Edad , Ligamento Periodontal/patologíaRESUMEN
In 20 subjects aged 20-29 years the position of the connective tissue attachment was determined and the atrophy of the alveolar process bone was assessed by means of an analysis of pantomograms. The radiological image of the paradontium was compared with the result of clinical examination. It was found that radiological diagnosis by means of pantomography may be a valuable supplementation of clinical examination in case of advanced parodontitis with loss of connective tissue attachment by at least 6 mm.