RESUMEN
A continuous ambulatory peritoneal dialysis patient developed eosinophilic peritonitis and was followed for 7 months. After 1 month, the peritonitis resolved, with a concomitant drop in percentage of hypodense eosinophils (Eos) recovered from peritoneal dialysate (PD) as well as a drop in fluid major basic protein levels. Blood eosinophil differential percentages were low, but the percentage of hypodense Eos in the blood tended to be relatively increased. Stool samples showed no evidence of parasitic infection, and epicutaneous skin tests were negative. Leukotriene C4 levels remained relatively constant as did white blood cell counts. Flow cytometric analysis of lymphocytes and granulocytes from PD and blood revealed high levels of CD23-positive lymphocytes.
Asunto(s)
Eosinofilia/patología , Diálisis Peritoneal Ambulatoria Continua/efectos adversos , Peritonitis/patología , Ribonucleasas , Adulto , Antígenos CD/análisis , Proteínas Sanguíneas/análisis , Proteínas en los Gránulos del Eosinófilo , Eosinofilia/etiología , Eosinofilia/inmunología , Femenino , Citometría de Flujo , Granulocitos/inmunología , Antígenos HLA-DR/análisis , Humanos , Inmunoglobulina E/análisis , Recuento de Leucocitos , Leucocitos/inmunología , Peritonitis/etiología , Peritonitis/inmunología , Radioinmunoensayo , SRS-A/análisisRESUMEN
A flow cytometric immunofluorescence assay (FIFA) was developed to detect antibodies to human immunodeficiency virus (HIV) using live cell indirect immunofluorescence and analysis by flow cytometry. A panel of 107 sera, previously tested for anti-HIV antibody with the Abbott Enzyme-Linked Immunosorbent Assay test and Western blot (WB), was rescreened by FIFA. Antibodies were tested on HIV-infected and uninfected H9 cells in the FIFA. Although ELISA results indicated seven false positive results by comparison with the WB, 46 of 46 FIFA positive results tested WB positive and 61 of 61 FIFA negative results were WB negative. The results of FIFA showed 100% sensitivity and 100% specificity compared with WB. This rapid, quantitative, relatively easy assay makes FIFA an alternate confirmatory test for the presence of HIV antibodies.
Asunto(s)
Citometría de Flujo , Técnica del Anticuerpo Fluorescente , Anticuerpos Anti-VIH/análisis , Ensayo de Inmunoadsorción Enzimática , Estudios de Evaluación como Asunto , Humanos , Sensibilidad y EspecificidadRESUMEN
Monoclonal antibodies (MoAbs) to the major gag core protein p27 and a viral protein p44 of type D retrovirus (SRV-2) were produced and used in the detection of SRV-2 antigens in infected Raji cells and in tissues from macaques with simian acquired immunodeficiency syndrome (SAIDS) and retroperitoneal fibromatosis (RF). Anti-p44 MoAb showed inhibition of syncytium formation by both SRV-1- and SRV-2-infected Raji cells.
Asunto(s)
Anticuerpos Monoclonales/biosíntesis , Antígenos Virales/análisis , Retrovirus de los Simios/inmunología , Animales , Anticuerpos Monoclonales/inmunología , Antígenos Virales/inmunología , Western Blotting , Línea Celular , Citometría de Flujo , Técnica del Anticuerpo Fluorescente , Productos del Gen gag , Hibridomas , Inmunohistoquímica , Pruebas de Neutralización , Proteínas de los Retroviridae/inmunologíaRESUMEN
Human T-lymphotropic virus type III (HTLV-III) or lymphadenopathy-associated virus (LAV) is tropic for human T cells with the helper-inducer phenotype, as defined by reactivity with monoclonal antibodies specific for the T4 molecule. Treatment of T4+ T cells with monoclonal antibodies to T4 antigen blocks HTLV-III/LAV binding, syncytia formation, and infectivity. Thus, it has been inferred that the T4 molecule itself is a virus receptor. In the present studies, the surfaces of T4+ T cells were labeled radioactively, and then the cells were exposed to virus. After the cells were lysed, HTLV-III/LAV antibodies were found to precipitate a surface protein with a molecular weight of 58,000 (58K). By blocking and absorption experiments, this 58K protein was identified as the T4 molecule. No cell-surface structures other than the T4 molecule were involved in the antibody-antigen complex formation. Two monoclonal antibodies, each reactive with a separate epitope of the T4 molecule, were tested for their binding capacities in the presence of HTLV-III/LAV. When HTLV-III/LAV was bound to T4+ T cells, the virus blocked the binding of one of the monoclonal antibodies, T4A (OKT4A), but not of the other, T4 (OKT4). When HTLV-III/LAV was internally radiolabeled and bound to T4+ T cells which were then lysed, a viral glycoprotein of 110K (gp110) coprecipitated with the T4 molecule. The binding of gp110 to the T4 molecule may thus be a major factor in HTLV-III/LAV tropism and may prove useful in developing therapeutic or preventive measures for the acquired immune deficiency syndrome.