RESUMEN

To facilitate the characterization of plant genes, the Cre-loxP site-specific recombination system was adapted to make reporter vectors for plant expression studies. This system allows promoter fragments to be cloned into a small vector (univector) and subsequently recombined in vitro with binary vectors containing different reporter genes precisely at near-perfect efficiency. We have constructed univector-adapted vectors with three reporters, beta-glucuronidase, luciferase, and green fluorescent protein, and a BASTA-resistance gene for selection of plant transformants. Expression in plants using the new system was validated by comparison to conventional reporter vectors. These new vectors are efficient and economical alternatives to the other plant reporter vectors currently available. The royalty-free Cre-loxP system serves as a platform for the future expansion of recombination-based cloning vectors for plant research.

Asunto(s)

Expresión Génica/genética , Genes Reporteros/genética , Vectores Genéticos/genética , Integrasas/genética , Plantas/genética , Proteínas Virales/genética , Arabidopsis/genética , Proteínas de Arabidopsis/genética , Secuencia de Bases , Clonación Molecular , Frutas/genética , Glucuronidasa/genética , Glucuronidasa/metabolismo , Glutatión Transferasa/genética , Glutatión Transferasa/metabolismo , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Integrasas/metabolismo , Luciferasas/genética , Luciferasas/metabolismo , Datos de Secuencia Molecular , Hojas de la Planta/genética , Plantas Modificadas Genéticamente , Regiones Promotoras Genéticas/genética , Proteínas Recombinantes de Fusión/genética , Proteínas Recombinantes de Fusión/metabolismo , Recombinación Genética , Intercambiadores de Sodio-Hidrógeno/genética , Transcripción Genética/genética , Proteínas Virales/metabolismo