RESUMEN
Gain of chromosome 18q and translocation t(14;18) are] frequently found in B-cell non-Hodgkin's lymphomas (B-NHL). Increased BCL2 transcription and BCL2 protein expression have been suggested to be the result of the gain. We utilized FISH, PCR and array CGH to study BCL2 and chromosome 18 copy number changes and rearrangements in 93 cases of B-NHL. BCL2 protein was expressed in >75% of the tumor cells in 92% of the cases by immunohistochemistry. Gain of BCL2 was associated with a 25% increase in BCL2 expression levels (immunoblotting), whereas t(14;18) resulted in a 55% increase in BCL2 levels compared to cases without BCL2 alterations. The tumor cell (spontaneous) apoptotic fractions were similar for the cases with different BCL2 genotypes. However, the normal cell apoptotic fractions were higher for the tumors with t(14;18) compared to the tumors without BCL2 alterations, while the tumors with gain of BCL2 only showed intermediate levels. Low-level gains of parts of chromosome 18 were found in 14 of the 38 B-NHL cases with t(14;18), with a consensus region 18pter-q21.33 that did not include the BCL2 gene. The 11 cases with 18q gain only showed a consensus region encompassing 18q21.2-18q21.32 and 18q21.33, which contain PMAIP1/MALT1 and BCL2, respectively.
Asunto(s)
Apoptosis/genética , Aberraciones Cromosómicas , Cromosomas Humanos Par 14 , Cromosomas Humanos Par 18 , Linfoma de Células B/genética , Proteínas Proto-Oncogénicas c-bcl-2/genética , Análisis Citogenético , Dosificación de Gen , Regulación Neoplásica de la Expresión Génica , Reordenamiento Génico , Humanos , Ganglios Linfáticos/patología , Linfoma de Células B/patología , Proteínas Proto-Oncogénicas c-bcl-2/análisis , Translocación GenéticaRESUMEN
Common variable immunodeficiency (CVID) represents a heterogeneous group of antibody deficiency syndromes, characterized by defective antibody production in which T cell deficiency may play a pathogenic role. A subgroup of CVID patients has impaired in vitro T cell proliferation. Using microarray analyses of T cells from these patients, we found a gene expression pattern different from healthy controls and patients with X-linked agammaglobulinaemia. The profile of the differentially expressed genes suggests enhanced cytotoxic effector functions, antigen experienced or chronically activated T cells and a predominance of CCR7(-) T cells. Further experiments using flow cytometry revealed a striking predominance of CCR7(-) T cells in a subgroup of CVID patients, and an association with impaired T cell proliferation. Our observations indicate that a predominance of CCR7(-) T cells with effector-memory cell features and with reduced proliferative capacity may characterize a subgroup of CVID.
Asunto(s)
Inmunodeficiencia Variable Común/inmunología , Regulación de la Expresión Génica/inmunología , Receptores de Quimiocina/inmunología , Linfocitos T/inmunología , Adulto , Agammaglobulinemia/genética , Agammaglobulinemia/inmunología , Anciano , División Celular/genética , División Celular/inmunología , Células Cultivadas , Cromosomas Humanos X , Inmunodeficiencia Variable Común/genética , Femenino , Regulación de la Expresión Génica/genética , Ligamiento Genético/genética , Ligamiento Genético/inmunología , Humanos , Memoria Inmunológica/inmunología , Activación de Linfocitos/genética , Activación de Linfocitos/inmunología , Masculino , Persona de Mediana Edad , Análisis de Secuencia por Matrices de Oligonucleótidos/métodos , Receptores CCR7 , Transcripción GenéticaRESUMEN
332 persons, who in 1987 received sanctions for sexual offences, were observed in freedom for five years, or until recidivism at an earlier stage. 82 (24.7%) committed new crimes during the observation period. Sexual offences comprised about 1/3 (8.4%) of the crimes committed. Rapists had the highest degree of recidivism. There was no significant difference between the various subgroups of sexual offenders (sexual offences against minors, rapists and other offenders) with regard to the frequency of recidivism into sexual offences. Registered sanctions for sexual offences before 1987, and sexual abuse and obscene conduct toward minors registered i 1987, were found to be significantly correlated to sexual recidivism after 1987. Problems regarding the evaluation of the effects of treatment to forestall recidivism into sexual crimes are discussed.
Asunto(s)
Delitos Sexuales , Femenino , Medicina Legal/legislación & jurisprudencia , Medicina Legal/estadística & datos numéricos , Psiquiatría Forense/legislación & jurisprudencia , Psiquiatría Forense/estadística & datos numéricos , Humanos , Masculino , Noruega , Recurrencia , Factores de RiesgoRESUMEN
Between 1980 and 1993, 26 patients were treated for primary lymphoma in the central nervous system at the Norwegian Radium Hospital. This is a rare disease with poor prognosis and thus represents a great therapeutic challenge. Immunocompromised, e.g. AIDS patients, are a group at high risk, but the incidence has increased among immunocompetent patients as well. Median patient age was 64 years; and none of the patients had any signs of immunodeficiency. 23 of the patients received radiation therapy. 13 of the patients received some form of chemotherapy. The overall median survival was 19 months. WHO performance status 0-2, unifocal lesion, absence of steroid dependency and normal serum levels of LDH were all associated with longer survival. Although complete remissions were achieved in most patients, relapses in the central nervous system were frequent.