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Hemophagocytic Lymphohistiocytosis (HLH) is a rare, highly aggressive syndrome involving dysregulated immune response. Most cases are secondary to underlying diseases including infections, autoimmune disorders and malignancies. The burden of disease of histoplasmosis and leishmaniosis associated with advanced HIV is still significant in low-and-middle income countries (LMIC). We present a case of histoplasmosis and leishmaniasis associated HLH in a man with an AIDS diagnosis.
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Síndrome de Inmunodeficiencia Adquirida , Histoplasmosis , Leishmaniasis Visceral , Linfohistiocitosis Hemofagocítica , Síndrome de Inmunodeficiencia Adquirida/complicaciones , Histoplasmosis/complicaciones , Histoplasmosis/diagnóstico , Histoplasmosis/tratamiento farmacológico , Humanos , Leishmaniasis Visceral/complicaciones , Leishmaniasis Visceral/diagnóstico , Leishmaniasis Visceral/tratamiento farmacológico , Linfohistiocitosis Hemofagocítica/diagnóstico , Linfohistiocitosis Hemofagocítica/etiología , MasculinoRESUMEN
ABSTRACT Background: Severe pneumonia is the most common cause of intensive care unit (ICU) admission and death due to novel coronavirus (SARS-CoV-2) respiratory disease (COVID-19). Due to its rapid outbreak, units for the evaluation of febrile patients in the pre-hospital setting were created. Objective: The objective of the study was to develop a sensitive and simple tool to assess the risk of pneumonia in COVID-19 patients and thus select which patients would require a chest imaging study. Materials and Methods: We conducted a cross-sectional study in a cohort of individuals with suspected COVID-19 evaluated in a public academic healthcare center in Buenos Aires city. All adult patients with positive RT-PCR assay for SARS-COV2 between April 24 and May 19 of 2020 were included in the study. Pneumonia was defined as the presence of compatible signs and symptoms with imaging confirmation. Univariate and multivariate logistic regression was performed. A risk indicator score was developed. Results: One hundred and forty-eight patients were included, 71 (48%) received the diagnosis of pneumonia. The final clinical model included four variables: age ≥ 40 years, cough, absence of sore throat, and respiratory rate ≥ 22. To create the score, we assigned values to the variables according to their ORs: 2 points for respiratory rate ≥ 22 and 1 point to the other variables. The AUC of the ROC curve was 0.80 (CI 95% 0.73-0.86). A cutoff value of 2 showed a sensitivity of 95.7% and a specificity of 43.24%. Conclusion: This sensible score may improve the risk stratification of COVID-19 patients in the pre-hospital setting. (REV INVEST CLIN. 2021;73(1):52-8)
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Humanos , Masculino , Femenino , Adolescente , Adulto , Persona de Mediana Edad , Adulto Joven , Neumonía Viral/diagnóstico , Fiebre/diagnóstico , COVID-19/complicaciones , Unidades de Cuidados Intensivos , Argentina , Neumonía Viral/etiología , Índice de Severidad de la Enfermedad , Riesgo , Estudios Transversales , Estudios Prospectivos , Estudios de Cohortes , Sensibilidad y Especificidad , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Fiebre/virología , COVID-19/diagnósticoRESUMEN
BACKGROUND: Cumulative burden of multiple mental health conditions may worsen physical health outcomes in vulnerable populations. Accordingly, identifying cumulative burdens of mental health conditions that may affect HIV treatment and care can guide public health strategies to reduce their impact on HIV-related health outcomes. This study examined the relationship between the cumulative burden of mental health conditions and factors associated with engagement in HIV care in Argentina. METHOD: Data for this study was obtained at baseline from Conexiones y Opciones Positivas en la Argentina 2 (COPA2). Participants (N = 360) were cisgender patients living with HIV who were lost to care, recruited from seven clinics serving people living with HIV in four Argentine urban centers. Cumulative burden of mental health conditions (i.e., depressive symptoms, problematic substance use, unhealthy alcohol use, and psychotic symptoms) was assessed. RESULTS: Every one-point increase in the number of mental health conditions present was associated with a decrement in patient-provider communication (b = - 0.22, p < .001), self-efficacy (b = - 0.13, p = .012), and motivation for adherence (b = - 0.11, p = .039). CONCLUSION: This study found cumulative burden of depression, problematic substance use, unhealthy alcohol use, and psychotic symptoms to be negatively associated with factors related to engagement in HIV care. Results highlight the importance of identification and treatment of challenges to mental health, in order to ameliorate their influence on engagement in HIV care.
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BACKGROUND: Severe pneumonia is the most common cause of intensive care unit (ICU) admission and death due to novel coronavirus (SARS-CoV-2) respiratory disease (COVID-19). Due to its rapid outbreak, units for the evaluation of febrile patients in the pre-hospital setting were created. OBJECTIVE: The objective of the study was to develop a sensitive and simple tool to assess the risk of pneumonia in COVID-19 patients and thus select which patients would require a chest imaging study. MATERIALS AND METHODS: We conducted a cross-sectional study in a cohort of individuals with suspected COVID-19 evaluated in a public academic healthcare center in Buenos Aires city. All adult patients with positive RT-PCR assay for SARS-COV2 between April 24 and May 19 of 2020 were included in the study. Pneumonia was defined as the presence of compatible signs and symptoms with imaging confirmation. Univariate and multivariate logistic regression was performed. A risk indicator score was developed. RESULTS: One hundred and forty-eight patients were included, 71 (48%) received the diagnosis of pneumonia. The final clinical model included four variables: age >- 40 years, cough, absence of sore throat, and respiratory rate >- 22. To create the score, we assigned values to the variables according to their ORs: 2 points for respiratory rate >- 22 and 1 point to the other variables. The AUC of the ROC curve was 0.80 (CI 95% 0.73-0.86). A cutoff value of 2 showed a sensitivity of 95.7% and a specificity of 43.24%. CONCLUSION: This sensible score may improve the risk stratification of COVID-19 patients in the pre-hospital setting.
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COVID-19/complicaciones , Fiebre/diagnóstico , Unidades de Cuidados Intensivos , Neumonía Viral/diagnóstico , Adolescente , Adulto , Argentina , COVID-19/diagnóstico , Estudios de Cohortes , Estudios Transversales , Femenino , Fiebre/virología , Humanos , Masculino , Persona de Mediana Edad , Neumonía Viral/etiología , Estudios Prospectivos , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Riesgo , Sensibilidad y Especificidad , Índice de Severidad de la Enfermedad , Adulto JovenRESUMEN
Limited information is available concerning the coexistence of COVID-19 and opportunistic infections in people living with HIV. The possible association of COVID-19 with AIDS-related respiratory diseases should be considered, particularly in patients with advance immunosuppression. We report the case of a male patient with AIDS-related disseminated histoplasmosis associated with COVID-19.
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Infecciones Oportunistas Relacionadas con el SIDA/complicaciones , Infecciones por VIH/complicaciones , Histoplasma/aislamiento & purificación , Histoplasmosis/diagnóstico , Infecciones Oportunistas Relacionadas con el SIDA/diagnóstico , Adulto , Recuento de Linfocito CD4 , Infecciones por VIH/tratamiento farmacológico , Histoplasmosis/complicaciones , Histoplasmosis/virología , Humanos , Terapia de Inmunosupresión , MasculinoRESUMEN
BACKGROUND: Hepatitis C virus (HCV) coinfection among people with human immunodeficiency virus (HIV) might perturb immune function and HIV persistence. We aimed to evaluate the impact of HCV clearance with direct-acting antivirals (DAAs) on immune activation and HIV persistence in HIV/HCV-coinfected individuals on antiretroviral therapy (ART). METHODS: In a prospective observational study, ART-treated participants with HIV/HCV coinfection received sofosbuvir/daclatasvirâ ±â ribavirin (nâ =â 19). Blood samples were collected before DAA therapy, at the end of treatment, and 12 months after DAA termination (12MPT). T- and natural killer (NK)-cell phenotype, soluble plasma factors, cell-associated (CA)-HIV deoxyribonucleic acid (DNA) forms (total, integrated, 2LTR), CA-unspliced (US) and multiple-spliced ribonucleic acid (RNA), and plasma HIV RNA were evaluated. RESULTS: Hepatitis C virus clearance was associated with (1) a downmodulation of activation and exhaustion markers in CD4+, CD8+ T, and NK cells together with (2) decreased plasma levels of Interferon gamma-induced protein 10 (IP10), interleukin-8 (IL-8), soluble (s)CD163 and soluble intercellular adhesion molecule (sICAM). Cell-associated US HIV RNA was significantly higher at 12MPT compared to baseline, with no change in HIV DNA or plasma RNA. CONCLUSIONS: Elimination of HCV in HIV/HCV-coinfected individuals alters immune function and the transcriptional activity of latently infected cells. This report provides insights into the effects of HCV coinfection in HIV persistence and regards coinfected subjects as a population in which HIV remission might prove to be more challenging.
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INTRODUCTION: HIV worsens HCV-related liver disease by accelerating fibrosis progression; however, progression rates are extremely variable among HIV/HCV-coinfected individuals. NK cells are associated with modulation of liver fibrosis and are profoundly altered during HCV and HIV infections. CD4+ T-cells modulate NK cell function, and are also affected by HIV infection. Here, we aim to characterize the association of hepatic fibrosis with both the phenotype and function of peripheral NK cells and their regulation by CD4+ T-cells, in HIV/HCV-coinfected individuals. METHODS: Thirty-four HIV/HCV-coinfected individuals with minimal (n = 16) and advanced (n = 18) fibrosis (METAVIR F0/F1 and F4 scores respectively) and 20 healthy volunteers were enrolled. PBMC were obtained from peripheral blood samples and NK and CD4+ T-cells were isolated and analysed. NK cell phenotype (CD25, CD69, Nkp46, NKG2D, PD-1), degranulation (CD107a) and IFN-γ and TNF-α production, as well as CD4+ T-cell activation (CD69, CD25 and CD38) were measured by flow cytometry. CD4+ T-cell conditioned medium (CM) derived from F0/F1 or F4 individuals was assessed for IL-2 levels by ELISA. Modulation of NK cell functionality by these CMs was also analysed. RESULTS: When comparing to NK cells from individuals with minimal fibrosis, degranulation and cytokine secretion by NK cells from subjects with F4 scores was significantly impaired, while PD-1 expression was augmented. On the one hand, neither the expression of activation markers nor IL-2 secretion was distinctly induced in CD4+ T-cells from subjects with F0/F1 or F4 METAVIR scores. Finally, NK cell degranulation and cytokine secretion were not differentially modulated by CD4+ T-cell CM, whether CD4+ T-cells derived from subjects with minimal or advanced fibrosis. CONCLUSIONS: Low levels of NK and CD4+ T-cells in HIV/HCV-coinfected individuals with advanced liver fibrosis have been previously described. Here, we show that advanced liver fibrosis in coinfected individuals is associated to a defective function of NK cells and an increased expression of the exhaustion/senescence marker PD-1. This NK signature could not be attributed to changes in the ability of CD4+ T-cells to modulate NK cell function.
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Linfocitos T CD4-Positivos/inmunología , Coinfección/inmunología , Infecciones por VIH/inmunología , Hepatitis C/inmunología , Células Asesinas Naturales/inmunología , Cirrosis Hepática/inmunología , Adulto , Anciano , Coinfección/complicaciones , Coinfección/virología , Femenino , Infecciones por VIH/complicaciones , Infecciones por VIH/virología , VIH-1/fisiología , Hepacivirus/fisiología , Hepatitis C/complicaciones , Hepatitis C/virología , Humanos , Leucocitos Mononucleares/inmunología , Cirrosis Hepática/complicaciones , Cirrosis Hepática/virología , Activación de Linfocitos , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
Objetivo: informar un caso de absceso cerebral odontogénico secundario a bacteriemia posraspaje radicular y revisar el tratamiento de esta patología poco frecuente. Caso clínico: un paciente de sexo masculino, de 57 años de edad, con antecedentes de diabetes tipo II mal controlada, fue intervenido mediante una limpieza gingival con cavitador ultrasónico, sin profilaxis antibiótica. A las 2 semanas, evolucionó con cefalea, fiebre y deterioro del sensorio. Al ingresar al hospital, se le realizó una resonancia cerebral que evidenció imagen compatible con absceso temporomesial izquierdo. Se efectuó la evacuación quirúrgica bajo guía estereotáxica y se rescató material purulento con cultivos positivos para Streptococcus milleri. Se indicó un tratamiento antibiótico con ceftriaxona y metronidazol. El paciente evolucionó con mejoría sintomática, sin déficit neurológico y sin lesión, según la imagen por resonancia magnética de control. Conclusión: los abscesos cerebrales constituyen una patología con alta morbimortalidad. Aunque el origen odontogénico es raro, es necesario evaluar a los pacientes con comorbilidades -como la inmunosupresión- y someterlos a prácticas habituales de bajo riesgo, a fin de minimizar probables complicaciones asociadas.
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Persona de Mediana Edad , Absceso Encefálico/etiología , Raspado Dental , Infección Focal Dental/complicaciones , Antibacterianos/uso terapéutico , Metronidazol/uso terapéuticoRESUMEN
Objetivo: informar un caso de absceso cerebral odontogénico secundario a bacteriemia posraspaje radicular y revisar el tratamiento de esta patología poco frecuente. Caso clínico: un paciente de sexo masculino, de 57 años de edad, con antecedentes de diabetes tipo II mal controlada, fue intervenido mediante una limpieza gingival con cavitador ultrasónico, sin profilaxis antibiótica. A las 2 semanas, evolucionó con cefalea, fiebre y deterioro del sensorio. Al ingresar al hospital, se le realizó una resonancia cerebral que evidenció imagen compatible con absceso temporomesial izquierdo. Se efectuó la evacuación quirúrgica bajo guía estereotáxica y se rescató material purulento con cultivos positivos para Streptococcus milleri. Se indicó un tratamiento antibiótico con ceftriaxona y metronidazol. El paciente evolucionó con mejoría sintomática, sin déficit neurológico y sin lesión, según la imagen por resonancia magnética de control. Conclusión: los abscesos cerebrales constituyen una patología con alta morbimortalidad. Aunque el origen odontogénico es raro, es necesario evaluar a los pacientes con comorbilidades -como la inmunosupresión- y someterlos a prácticas habituales de bajo riesgo, a fin de minimizar probables complicaciones asociadas.(AU)