RESUMEN
Pearl millet [ (L.) R. Br; also (L.) Morrone] is an important crop throughout the world but better genomic resources for this species are needed to facilitate crop improvement. Genome mapping studies are a prerequisite for tagging agronomically important traits. Genotyping-by-sequencing (GBS) markers can be used to build high-density linkage maps, even in species lacking a reference genome. A recombinant inbred line (RIL) mapping population was developed from a cross between the lines 'Tift 99DB' and 'Tift 454'. DNA from 186 RILs, the parents, and the F was used for 96-plex KI GBS library development, which was further used for sequencing. The sequencing results showed that the average number of good reads per individual was 2.2 million, the pass filter rate was 88%, and the CV was 43%. High-quality GBS markers were developed with stringent filtering on sequence data from 179 RILs. The reference genetic map developed using 150 RILs contained 16,650 single-nucleotide polymorphisms (SNPs) and 333,567 sequence tags spread across all seven chromosomes. The overall average density of SNP markers was 23.23 SNP/cM in the final map and 1.66 unique linkage bins per cM covering a total genetic distance of 716.7 cM. The linkage map was further validated for its utility by using it in mapping quantitative trait loci (QTLs) for flowering time and resistance to leaf spot [ (Cke.) Sacc.]. This map is the densest yet reported for this crop and will be a valuable resource for the pearl millet community.
Asunto(s)
Resistencia a la Enfermedad/genética , Pennisetum/genética , Análisis de Secuencia de ADN , Mapeo Cromosómico , Ligamiento Genético , Genoma de Planta , Genotipo , Técnicas de Genotipaje/métodos , Polimorfismo de Nucleótido Simple , Sitios de Carácter Cuantitativo/genéticaRESUMEN
Soil carbon (C) sequestration in tilled and nontilled areas can be influenced by crop management practices due to differences in plant C inputs and their rate of mineralization. We examined the influence of four cover crops {legume [hairy vetch (Vicia villosa Roth)], nonlegume [rye (Secale cereale L.)], biculture of legume and nonlegume (vetch and rye), and no cover crops (or winter weeds)} and three nitrogen (N) fertilization rates (0, 60 to 65, and 120 to 130 kg N ha(-1)) on C inputs from cover crops, cotton (Gossypium hirsutum L.), and sorghum [Sorghum bicolor (L.) Moench)], and soil organic carbon (SOC) at the 0- to 120-cm depth in tilled and nontilled areas. A field experiment was conducted on Dothan sandy loam (fine-loamy, siliceous, thermic Plinthic Paleudults) from 1999 to 2002 in central Georgia. Total C inputs to the soil from cover crops, cotton, and sorghum from 2000 to 2002 ranged from 6.8 to 22.8 Mg ha(-1). The SOC at 0 to 10 cm fluctuated with C input from October 1999 to November 2002 and was greater from cover crops than from weeds in no-tilled plots. In contrast, SOC values at 10 to 30 cm in no-tilled and at 0 to 60 cm in chisel-tilled plots were greater for biculture than for weeds. As a result, C at 0 to 30 cm was sequestered at rates of 267, 33, -133, and -967 kg C ha(-1) yr(-1) for biculture, rye, vetch, and weeds, respectively, in the no-tilled plot. In strip-tilled and chisel-tilled plots, SOC at 0 to 30 cm decreased at rates of 233 to 1233 kg C ha(-1) yr(-1). The SOC at 0 to 30 cm increased more in cover crops with 120 to 130 kg N ha(-1) yr(-1) than in weeds with 0 kg N ha(-1) yr(-1), regardless of tillage. In the subtropical humid region of the southeastern United States, cover crops and N fertilization can increase the amount of C input and storage in tilled and nontilled soils, and hairy vetch and rye biculture was more effective in sequestering C than monocultures or no cover crop.
Asunto(s)
Carbono/metabolismo , Productos Agrícolas/crecimiento & desarrollo , Fertilizantes , Nitrógeno/metabolismo , Compuestos Orgánicos/metabolismo , Suelo/análisis , Agricultura/métodos , Monitoreo del Ambiente , Georgia , Compuestos Orgánicos/química , Factores de TiempoRESUMEN
In the past several decades, agricultural management practices consisting of intensive tillage and high rate of fertilization to improve crop yields have resulted in the degradation of soil and environmental qualities by increasing erosion and nutrient leaching in the groundwater and releasing greenhouses gases, such as carbon dioxide (CO2) and nitrous oxide (N2O), that cause global warming in the atmosphere by oxidation of soil organic matter. Consequently, management practices that sustain crop yields and improve soil and environmental qualities are needed. This paper reviews the findings of the effects of tillage practices, cover crops, and nitrogen (N) fertilization rates on crop yields, soil organic carbon (C) and N concentrations, and nitrate (NO3)-N leaching from the soil. Studies indicate that conservation tillage, such as no-till or reduced till, can increase soil organic C and N concentrations at 0- to 20-cm depth by as much as 7-17% in 8 years compared with conventional tillage without significantly altering crop yields. Similarly, cover cropping and 80-180 kg N ha(-1) year(-1) fertilization can increase soil organic C and N concentrations by as much as 4-12% compared with no cover cropping or N fertilization by increasing plant biomass and amount of C and N inputs to the soil. Reduced till, cover cropping, and decreased rate of N fertilization can reduce soil N leaching compared with conventional till, no cover cropping, and full rate of N fertilization. Management practices consisting of combinations of conservation tillage, mixture of legume and nonlegume cover crops, and reduced rate of N fertilization have the potentials for sustaining crop yields, increasing soil C and N storage, and reducing soil N leaching, thereby helping to improve soil and water qualities. Economical and social analyses of such practices are needed to find whether they are cost effective and acceptable to the farmers.