RESUMEN

The yeast YCC5 gene encodes a putative amino acid permease and is homologous to GNP1 (encoding a high-affinity glutamine permease). Using strains with disruptions in the genes for multiple permeases, we demonstrated that Ycc5 (which we have renamed Agp1) is involved in the transport of asparagine and glutamine, performed a kinetic analysis of this activity, and showed that AGP1 expression is subject to nitrogen repression.

Asunto(s)

Sistemas de Transporte de Aminoácidos Básicos , Asparagina/metabolismo , Genes Fúngicos , Glutamina/metabolismo , Proteínas de Transporte de Membrana/genética , Saccharomyces cerevisiae/genética , Sistemas de Transporte de Aminoácidos , Sistemas de Transporte de Aminoácidos Neutros , Aminoácidos Dicarboxílicos/farmacología , Amoníaco/farmacología , Transporte Biológico , Regulación Fúngica de la Expresión Génica/efectos de los fármacos , Cinética , Proteínas de Transporte de Membrana/metabolismo , Proteínas de Saccharomyces cerevisiae