RESUMEN
Small populations of imperiled species are susceptible to the negative consequences of skewed sex-ratios. In imperiled species with environmental sex determination such as sea turtles, examining sex ratios across a range of environments and population abundance levels can provide insight into factors that influence population resilience, which can then be the foci of management plans for these species. Breeding sex ratios (the ratio of actively breeding males to females during a reproductive season; BSRs) extrapolated from genetic parentage analyses are a common approach for enumerating sex ratios in sea turtles. Such analyses also allow for the characterization of multiple paternity within sea turtle clutches, which should reflect BSRs and breeding behaviors. We characterized the first BSR for a breeding assemblage of loggerhead sea turtles (Caretta caretta) belonging to the temperate, low-abundance Northern Gulf of Mexico Recovery Unit using genotypes of 16 microsatellite loci from nesting females and hatchlings. Unlike prior studies at both more-tropical and more-temperate, and higher-abundance, Recovery Units in this region, we found a balanced BSR of 1.3:1 males:female and a low incidence (~17%) of multiple paternity. This suggests that there are relatively few males breeding at this assemblage and within this Recovery Unit. Beaches in this region are expected to produce substantial numbers of male hatchlings based on sand temperature data. The relative dearth of mature males may then be due to hydrologic disturbances that disproportionately affect the fitness and survival of male hatchlings, or due to demographic stochasticity. More work is needed to study the factors that might influence male hatchling production and fitness in this region, particularly as climate change is predicted to lead to feminization in global sea turtle populations. Our work demonstrates the broad utility of characterizing BSRs and other sex ratios across a range of populations in imperiled, environmentally sensitive species.
RESUMEN
A species may partition its realized ecological niche along bionomic and scenopoetic axes due to intraspecific competition for limited resources. How partitioning manifests depends on resource needs and availability by and for the partitioning groups. Here we demonstrate the utility of analysing short- and long-term stable carbon and nitrogen isotope ratios from imperiled marine megafauna to characterize realized niche partitioning in these species. We captured 113 loggerhead sea turtles (Caretta caretta) at a high-use area in the eastern Big Bend, Florida, between 2016 and 2022, comprising 53 subadults, 10 adult males and 50 adult females. We calculated trophic niche metrics using established and novel methods, and constructed Bayesian ellipses and hulls, to characterize loggerhead isotopic niches. These analyses indicated that loggerheads partition their realized ecological niche by lifestage, potentially along both bionomic (e.g. trophic) and/or scenopoetic (e.g. habitat, latitude or longitude) axes, and display different characteristics of resource use within their niches. Analysis of stable isotopes from tissues with different turnover rates enabled this first characterization of intraspecific niche partitioning between and within neritic lifestages in loggerhead turtles, which has direct implications for ongoing research and conservation efforts for this and other imperiled marine species.
RESUMEN
Conservation of green sea turtles (Chelonia mydas) benefits from knowledge of population connectivity across life stages. Green turtles are managed at the level of genetically discrete rookeries, yet individuals from different rookeries mix at foraging grounds; therefore, rookeries may be impacted by processes at foraging grounds. Bimini, Bahamas, hosts an important foraging assemblage, but rookery contributions to this assemblage have never been resolved. We generated mitochondrial DNA sequences for 96 foraging green turtles from Bimini and used Mixed Stock Analysis to determine rookery contributions to this population using 817 and 490 base pair (bp) rookery baseline data. The MSA conducted with 817 bp data indicated that Quintana Roo, Mexico, and Central Eastern Florida contributed most to the Bimini population. The MSA conducted with 490 bp data indicated that Southwest Cuba and Central Eastern Florida contributed the most to Bimini. The results of the second MSA differ from a previous study undertaken with 490 bp data, conducted in Great Inagua, Bahamas, which suggested that Tortuguero, Costa Rica, contributed the most to that foraging assemblage. Large credible intervals in our results do not permit explicit interpretation of individual rookery contributions, but our results do indicate substantial relative differences in rookery contributions to two Bahamian foraging assemblages which may be driven by oceanic currents, rookery sizes, and possibly juvenile natal homing. Our findings may implicate a shift in contributions to the Bahamas over two decades, highlighting the importance of regularly monitoring rookery contributions and resolving regional recruitment patterns to inform conservation.
RESUMEN
Endangered species are grouped into genetically discrete populations to direct conservation efforts. Mitochondrial control region (mtCR) haplotypes are used to elucidate deep divergences between populations, as compared to nuclear microsatellites that can detect recent structuring. When prior populations are unknown, it is useful to subject microsatellite data to clustering and/or ordination population inference. Olive ridley sea turtles (Lepidochelys olivacea) are the most abundant sea turtle, yet few studies have characterized olive ridley population structure. Recently, clustering results of olive ridleys in the Eastern Tropical Pacific Ocean suggested weak structuring (F ST = 0.02) between Mexico and Central America. We analyzed mtCR haplotypes, new microsatellite genotypes from Costa Rica, and preexisting microsatellite genotypes from olive ridleys across the Eastern Tropical Pacific, to further explore population structuring in this region. We subjected inferred populations to multiple analyses to explore the mechanisms behind their structuring. We found 10 mtCR haplotypes from 60 turtles nesting at three sites in Costa Rica, but did not detect divergence between Costa Rican sites, or between Central America and Mexico. In Costa Rica, clustering suggested one population with no structuring, but ordination suggested four cryptic clusters with moderate structuring (F ST = 0.08, p < .001). Across the Eastern Tropical Pacific, ordination suggested nine cryptic clusters with moderate structuring (F ST = 0.103, p < .001) that largely corresponded to Mexican and Central American populations. All ordination clusters displayed significant internal relatedness relative to global relatedness (p < .001) and contained numerous sibling pairs. This suggests that broadly dispersed family lineages have proliferated in Eastern Tropical Pacific olive ridleys and corroborates previous work showing basin-wide connectivity and shallow population structure in this region. The existence of broadly dispersed kin in Eastern Tropical Pacific olive ridleys has implications for management of olive ridleys in this region, and adds to our understanding of sea turtle ecology and life history, particularly in light of the natal-homing paradigm.