RESUMEN
Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential relationship between biochemical characteristics and quinolone resistance in uropathogenic E. coli strains. The present work analyzed the quinolone sensitivity and biochemical activities of fifty-eight lactose-negative uropathogenic E. coli strains. A high percentage of the isolates (48.3%) was found to be resistant to at least one of the tested quinolones, and DNA sequencing revealed quinolone resistant determining region gyrA and parC mutations in the multi-resistant isolates. Statistical analyses suggested that the lack of ornithine decarboxylase (ODC) activity is correlated with quinolone resistance. Despite the low number of isolates examined, this is the first study correlating these characteristics in lactose-negative E. coli isolates.
Asunto(s)
Farmacorresistencia Bacteriana Múltiple/genética , Infecciones por Escherichia coli/tratamiento farmacológico , Fluoroquinolonas/uso terapéutico , Lactosa/metabolismo , Ácido Nalidíxico/uso terapéutico , Ornitina Descarboxilasa/genética , Infecciones Urinarias/tratamiento farmacológico , Escherichia coli Uropatógena/genética , Antibacterianos/uso terapéutico , Brasil , Girasa de ADN/genética , Topoisomerasa de ADN IV/genética , Descarboxilación/genética , Descarboxilación/fisiología , Infecciones por Escherichia coli/microbiología , Humanos , Pruebas de Sensibilidad Microbiana , Ornitina/metabolismo , Infecciones Urinarias/microbiología , Escherichia coli Uropatógena/efectos de los fármacos , Escherichia coli Uropatógena/enzimología , Escherichia coli Uropatógena/aislamiento & purificaciónRESUMEN
Quinolones and fluoroquinolones are widely used to treat uropathogenic Escherichia coli infections. Bacterial resistance to these antimicrobials primarily involves mutations in gyrA and parC genes. To date, no studies have examined the potential relationship between biochemical characteristics and quinolone resistance in uropathogenic E. coli strains. The present work analyzed the quinolone sensitivity and biochemical activities of fifty-eight lactose-negative uropathogenic E. coli strains. A high percentage of the isolates (48.3%) was found to be resistant to at least one of the tested quinolones, and DNA sequencing revealed quinolone resistant determining region gyrA and parC mutations in the multi-resistant isolates. Statistical analyses suggested that the lack of ornithine decarboxylase (ODC) activity is correlated with quinolone resistance. Despite the low number of isolates examined, this is the first study correlating these characteristics in lactose-negative E. coli isolates.(AU)
Asunto(s)
Humanos , Farmacorresistencia Bacteriana Múltiple/genética , Infecciones por Escherichia coli/tratamiento farmacológico , Fluoroquinolonas/uso terapéutico , Lactosa/metabolismo , Ácido Nalidíxico/uso terapéutico , Ornitina Descarboxilasa/genética , Escherichia coli Uropatógena/genética , Antibacterianos/uso terapéutico , Brasil , Girasa de ADN/genética , Topoisomerasa de ADN IV/genética , Descarboxilación/genética , Descarboxilación/fisiología , Infecciones por Escherichia coli/microbiología , Pruebas de Sensibilidad Microbiana , Ornitina/metabolismo , /microbiología , Escherichia coli Uropatógena , Escherichia coli Uropatógena/enzimología , Escherichia coli Uropatógena/aislamiento & purificaciónRESUMEN
Quinolones and fluoroquinolones are widely used to treat uropathogenic