RESUMEN
Currently, the research of new natural compounds with biological potential demonstrates great ethnopharmacological importance. In this study, we evaluated the biological properties promoted by saline extract from Malpighia emarginata DC leaves, whose objective is to evaluate the antioxidant, antimicrobial and cytotoxicity potential. Phytochemical characterization was performed by UPLC-MS chromatography to identify the chemical compounds. For the antioxidant potential, DPPH, ATT and FRAP methods were used. The antibacterial and antifungal tests were performed evaluating the MIC50, MIC90, CMB and CMF parameters. Moreover, antibiofilm action was evaluated. Cytotoxicity and proliferation were performed using splenocytes from Balb/c mice and were evaluated by cytometry. We found a list of phenolic compounds among other bioactive compounds in the M. emarginata saline extract. In addition, higher antioxidant profile and antifungal activity against different strains of Candida spp. was promoted by the saline extract. Splenocytes showed greater cell viability (more than 90%) and showed higher proliferate index in 24 and 48 hours of incubation with the extract. Saline extract from Malpighia emarginata DC has potential action like antioxidant and antifungal agent without promote animal cell damage.
Asunto(s)
Antifúngicos/química , Antioxidantes/química , Proliferación Celular/efectos de los fármacos , Malpighiaceae/química , Extractos Vegetales/química , Hojas de la Planta/química , Polifenoles/química , Animales , Antifúngicos/farmacología , Antioxidantes/farmacología , Candida/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Cromatografía Líquida de Alta Presión/métodos , Femenino , Flavonoides/química , Ratones Endogámicos BALB C , Pruebas de Sensibilidad Microbiana , Extractos Vegetales/farmacología , Polifenoles/farmacología , Valores de Referencia , Bazo/citología , Factores de TiempoRESUMEN
In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection 'University Recife Mycologia' (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.
Asunto(s)
Endófitos/metabolismo , Hongos/metabolismo , Microbiología/organización & administración , Paclitaxel/biosíntesis , Endófitos/genética , Hongos/genética , Preservación BiológicaRESUMEN
Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection 'University Recife Mycologia' (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.
Asunto(s)
Paclitaxel/biosíntesis , Endófitos/metabolismo , Hongos/metabolismo , Microbiología/organización & administración , Preservación Biológica , Endófitos/genética , Hongos/genéticaRESUMEN
In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection University Recife Mycologia (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.(AU)
RESUMEN
Abstract In this work, four isolates of endophytic fungi (Alternaria alternata, Colletotrichum gloesporioides, Glomerella cingulata and Nigrospora sphaerica), deposited in the culture collection University Recife Mycologia (URM) at the Universidade Federal de Pernambuco, were characterized for the genes ITS 1 and 4 (region 5.8 S) and evaluated for taxol production.