RESUMEN
Silicon (Si) plays important roles in alleviating various abiotic stresses. In rice (Oryza sativa), arsenic (As) is believed to share the Si transport pathway for entry into roots, and Si has been demonstrated to decrease As concentrations. However, the physiological mechanisms through which Si might alleviate As toxicity in plants remain poorly elucidated. We combined detailed gas exchange measurements with chlorophyll fluorescence analysis to examine the effects of Si nutrition on photosynthetic performance in rice plants [a wild-type (WT) cultivar and its lsi1 mutant defective in Si uptake] challenged with As (arsenite). As treatment impaired carbon fixation (particularly in the WT genotype) that was unrelated to photochemical or biochemical limitations but, rather, was largely associated with decreased leaf conductance at the stomata and mesophyll levels. Indeed, regardless of the genotypes, in the plants challenged with As, photosynthetic rates correlated strongly with both stomatal (r(2) = 0.90) and mesophyll (r(2) = 0.95) conductances, and these conductances were, in turn, linearly correlated with each other. The As-related impairments to carbon fixation could be considerably reverted by Si in a time- and genotype-dependent manner. In conclusion, we identified Si nutrition as an important target in an attempt to not only decrease As concentrations but also to ameliorate the photosynthetic performance of rice plants challenged with As.
Asunto(s)
Arsénico/toxicidad , Oryza/fisiología , Fotosíntesis/efectos de los fármacos , Hojas de la Planta/fisiología , Silicio/farmacología , Análisis de Varianza , Dióxido de Carbono/metabolismo , Difusión , Genotipo , Oryza/efectos de los fármacos , Oryza/genética , Hojas de la Planta/efectos de los fármacos , Estomas de Plantas/efectos de los fármacos , Estomas de Plantas/fisiología , Factores de TiempoRESUMEN
The effects of varying intensities of light on plants depend on when they occur, even if the total amount of light received is kept constant. We designed an experiment using two clones of robusta coffee (Coffea canephora) intercropped with shelter trees in such a way that allowed us to compare coffee bushes shaded in the morning (SM) with those shaded in the afternoon (SA), and then confronting both with bushes receiving full sunlight over the course of the day (FS). The SM bushes displayed better gas-exchange performance than their SA and FS counterparts, in which the capacity for CO2 fixation was mainly constrained by stomatal (SA bushes) and biochemical (FS bushes) factors. Physiological traits associated with light capture were more responsive to temporal fluctuations of light rather than to the amount of light received, although this behavior could be a clone-specific response. The activity of key antioxidant enzymes differed minimally when comparing the SM and SA clones, but was much larger in FS clones. No signs of photoinhibition or cell damage were found regardless of the light treatments. Acclimations to varying light supplies had no apparent additional cost for constructing and maintaining the leaves regardless of the light supply. Both the SM and SA individuals displayed higher return in terms of revenue streams (e.g. higher mass-based light-saturated photosynthetic rates, photosynthetic nitrogen use efficiencies and long-term water use efficiencies) than their FS counterparts. In conclusion, shading may improve the physiological performance of coffee bushes growing in harsh, tropical environments.