RESUMEN
INTRODUCTION: In orthodontic treatment, an increased bone mineral density of the alveolar bone is considered as a risk factor for apical root resorption (ARR), whereas the mineral density of cementum has been associated with root protection against resorption. METHODS: This study aimed at evaluating the grey values (GVs) of the apical third of the root and of the alveolar bone adjacent to maxillary incisors with and without ARR in orthodontic patients. Twenty-one patients under treatment who presented one incisor with ARR and its corresponding contralateral without ARR were selected and submitted to cone-beam computed tomography. GVs were evaluated on the images obtained of four areas of the apical third of the root and of four areas of the adjacent alveolar bone. RESULTS AND CONCLUSIONS: The radicular tissue of the apical third of the incisors with ARR showed greater root GV (p < .05) than that of the incisors without ARR. Supra-apical alveolar bone exhibited greater GV in the incisors without ARR than incisors with ARR (p < .05). Root GV was not associated with root protection, but rather seemed to have facilitated the process of resorption. The GV of the lingual bone was associated with a higher progression of ARR in the incisors.
Asunto(s)
Incisivo , Resorción Radicular , Proceso Alveolar , Tomografía Computarizada de Haz Cónico , Humanos , Maxilar , Raíz del DienteRESUMEN
INTRODUCTION: The diagnosis of apical root resorption is usually based on routine radiographs. However, these methods are limited because the images reflect the superimposition of the whole root structure and can lead to underestimation of the extent of apical root resorption. In this study, we aimed to determine the lengths of the labial and lingual surfaces of incisors with apical root resorption and compare them with the longest radicular length obtained on sagittal images of cone-beam computed tomography, and to create a qualitative visual scale of the different patterns of apical root resorption. METHODS: Eighty-two incisors with apical root resorption from 25 patients had their labial and lingual root surfaces and the longest radicular lengths determined in the sagittal plane and compared. Five orthodontists, at 2 times, classified the images of each incisor according to a visual scale developed by the authors. RESULTS: There was no significant difference between the labial and lingual surfaces; however, the longest radicular length was significantly greater than the shortest surface length. The visual scale showed intraobserver agreement of 0.615 and interobserver agreements of 0.74 and 0.52 at both times, respectively. CONCLUSIONS: The difference between the longest and shortest root lengths suggests that radiographic superimposition underestimates the extent of the resorption lesion. The proposed visual scale showed a frequency of agreement above 65% and a coefficient of reproducibility varying from moderate to substantial.
Asunto(s)
Tomografía Computarizada de Haz Cónico/métodos , Incisivo/diagnóstico por imagen , Odontometría/métodos , Resorción Radicular/diagnóstico por imagen , Ápice del Diente/diagnóstico por imagen , Adolescente , Adulto , Femenino , Estudios de Seguimiento , Humanos , Masculino , Intensificación de Imagen Radiográfica , Reproducibilidad de los Resultados , Técnicas de Movimiento Dental/instrumentación , Raíz del Diente/diagnóstico por imagen , Adulto JovenRESUMEN
OBJECTIVES: The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. MATERIAL AND METHODS: Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. RESULTS: Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. CONCLUSIONS: The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.
Asunto(s)
Periodontitis Agresiva/metabolismo , Periodontitis Crónica/metabolismo , Lactoferrina/análisis , Mucina 2/análisis , Saliva/química , Glándulas Salivales/metabolismo , Adulto , Western Blotting , Estudios de Casos y Controles , Electroforesis en Gel de Poliacrilamida , Femenino , Humanos , Lactoferrina/metabolismo , Masculino , Persona de Mediana Edad , Mucina 2/metabolismo , Tasa de Secreción , Factores de TiempoRESUMEN
OBJECTIVES:The aim of this study was to compare the pattern of secretion and the expression of mucin glycoprotein-2 (MG2) and lactoferrin in individuals with or without periodontitis. MATERIAL AND METHODS: Five individuals with aggressive periodontitis (APG), 5 with generalized chronic periodontitis (CPG) and 5 without periodontitis (CG) were enrolled after informed consent. Non-stimulated and stimulated submandibular and sublingual saliva was collected and samples analyzed by Western blot probed with specific antibodies. RESULTS: Stimulated and non-stimulated salivary flow rates did not differ among groups. Western blot analysis revealed that stimulation led to: an increase in MG2 expression in all groups, and to lactoferrin expression in APG and CPG. In non-stimulated saliva, CG exhibited the highest expression of both glycoproteins. In stimulated saliva, CG exhibited the highest expression of MG2, whereas APG the highest of lactoferrin. CONCLUSIONS: The pattern of secretion of MG2 and lactoferrin in health and disease is complex. Although the present study analyzed samples from a limited number of participants, the reduced expression of MG2 and lactoferrin in APG and CPG under non-stimulated condition, the predominant circumstance of salivary secretion during the day, suggests that these salivary constituents may play a role in the etiopathogenesis of these diseases.