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OBJECTIVES: The aim of this study was to investigate the genetic context of expanded-spectrum ß-lactam resistance in a Klebsiella pneumoniae strain causing a hard-to-treat nasal infection in a domestic cat. METHODS: A K. pneumoniae isolate was recovered from a 4-year-old male cat hospitalised in a veterinary hospital in Paraíba, Northeastern Brazil. Following phenotypic confirmation of multidrug resistance by the disk diffusion method, the genome was sequenced using an Illumina MiSeq system. Multilocus sequence typing (MLST) and structural features related to antimicrobial resistance were determined by downstream bioinformatics analyses. RESULTS: The strain was confirmed as sequence type 273 (ST273) K. pneumoniae harbouring a variety of genes conferring antimicrobial resistance to phenicols tetracyclines, aminoglycosides, ß-lactams, fosfomycin, sulfonamides and quinolones. Two plasmids were identified. Plasmid p114PB_I co-harboured a set of plasmid-borne resistance genes [blaCTX-M-15, blaTEM-1, qnrS1, tetD, tetR, sul2, aph(6)-Id, aph(3'') and cat2]. Notably, the multiresistance region was characterised as a chimeric plasmid structure sharing high sequence homology with several plasmids from Enterobacteriaceae. The second plasmid (p114PB_II) was characterised as a plasmid present in many genomes belonging to K. pneumoniae. CONCLUSION: The genetic context of the plasmid sequences harboured by a veterinary pathogenic K. pneumoniae isolate reveals the high complexity of horizontal gene transfer mechanisms in the acquisition of antimicrobial resistance genes. The emergence, dissemination and evolution of antimicrobial resistance must be investigated from a One Health perspective.

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OBJETIVO: Avaliar as oficinas como uma estratégia de intervenção nutricional, com foco na aquisição de informações em nutrição e na percepção de mudanças de hábitos alimentares. MÉTODOS: Estudo de intervenção com usuários de uma Academia da Cidade de Belo Horizonte, Minas Gerais. Realizaram-se quatro oficinas, com frequência mensal e duração de trinta minutos cada uma, pautadas no "Guia Alimentar para a População Brasileira" e nos "Dez Passos para uma Alimentação Saudável". Aplicaram-se questionários para avaliar a aquisição de informações em nutrição antes e depois da execução das oficinas, investigar a percepção de mudanças de hábitos alimentares após três meses da intervenção e identificar as principais dificuldades verificadas nesse processo. Um observador apontou a viabilidade de execução das oficinas. RESULTADOS: O número de participantes das oficinas variou de 71 a 132. Houve maior ocorrência (80%) de mulheres, com média de 55,8 (±10,6) anos. Identificou-se incremento estatisticamente significante na média total de acertos das questões do questionário de avaliação da aquisição de informações em três dos quatro encontros. Ademais, 90,9% dos participantes referiram alguma mudança de hábito alimentar, destacando-se a redução no consumo de óleos, gorduras e frituras (29,5%) e o aumento na ingestão de frutas e hortaliças (22,5%). A redução no consumo de açúcares e doces foi apontada como a recomendação mais difícil de ser seguida (21,1%). A avaliação do observador denotou a necessidade de modificar o espaço utilizado para as atividades. CONCLUSÃO: A intervenção apresentou potencial para aquisição de informações em nutrição e mudança de hábitos alimentares, alterações que devem ser mais bem investigadas com a continuidade das ações...

OBJECTIVE: To assess workshops as a nutritional intervention strategy, focusing on the transmission of nutrition information and perception of dietary changes. METHODS: The participants of this intervention study were users of a public gym (Academia da Cidade) in Belo Horizonte Minas Gerais. Four 30-minute workshops were held, one a month, based on the Brazilian Food Guide and Ten Steps to Healthy Eating. Questionnaires were administered to assess the participants' nutrition knowledge before and after the workshops, investigate perceived changes in dietary habits three months after the intervention, and identify the main difficulties associated with changing eating habits. One observer found the workshops viable. RESULTS: The number of participants in the workshops ranged from 71 to 132. The mean age of participants was 55.8±10.6 years, and 80% were females. The number of correct answers in the questionnaires increased significantly in three of the four workshops. Moreover, 90.9% of the participants referred changing at least one eating habit, especially reducing the intake of oils, fats, and deep-fried foods (29.5%), and increasing the intake of fruits and non-starchy vegetables (22.5%). Reducing the intake of sugar and sweets was reported as the hardest recommendation to follow (21.1%). The observer believes another area should be made available for the activities because, among others, the current area was too noisy. CONCLUSION: The intervention has the potential of increasing participants' knowledge of nutrition and promoting dietary changes. These changes need to be better assessed as the workshops continue...

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