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Mesenchymal stromal cells (MSCs) have long been used in research for bone regeneration, with evidence of their beneficial properties. In the segmental area of MSC-based therapies, MSC-derived extracellular vesicles (EVs) have also shown great therapeutic effects in several diseases, including bone healing. This study aimed to assess whether the conditioning of MSCs improves the therapeutic effects of their derived extracellular vesicles for bone regeneration. Electronic research was performed until February 2021 to recover the studies in the following databases: PubMed, Scopus, and Web of Science. The studies were screened based on the inclusion criteria. Relevant information was extracted, including in vitro and in vivo experiments, and the animal studies were evaluated for risk of bias by the SYRCLE tool. A total of 463 studies were retrieved, and 18 studies met the inclusion criteria (10 studies for their in vitro analysis, and 8 studies for their in vitro and in vivo analysis). The conditioning methods reported included: osteogenic medium; dimethyloxalylglycine; dexamethasone; strontium-substituted calcium silicate; hypoxia; 3D mechanical microenvironment; and the overexpression of miR-375, bone morphogenetic protein-2, and mutant hypoxia-inducible factor-1α. The conditioning methods of MSCs in the reported studies generate exosomes able to significantly promote bone regeneration. However, heterogeneity regarding cell source, conditioning method, EV isolation and concentration, and defect model was observed among the studies. The different conditioning methods reported in this review do improve the therapeutic effects of MSC-derived EVs for bone regeneration, but they still need to be addressed in larger animal models for further clinical application.

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Background: The urachus is a tubular structure continuous with the urinary bladder and the allantois in foetal mammals.It serves as a communication between these two structures. At birth, it loses its function and undergoes atrophy by fibrousproliferation within the lumen. When this atrophy process fails at some point, congenital anomalies of the urachus occur.These anomalies are rare in animals, and to our knowledge, the urachal cyst has not yet been described in dogs. The presentwork is unique in that it reports a case of this congenital defect in a dog. Our aim is to increase awareness and to discussthe clinical presentation, the imaging techniques used, and the final diagnosis of this anomaly.Case: A 3-year-old bitch Pit Bull was presented for veterinary assistance to investigate recurrent pseudopregnancy and anirregular estrous cycle. The animal presented in good bodily condition, and the white blood count and clinical biochemistrywere normal. At ultrasound, 2 tubular structures, filled by an echogenic fluid mimicking uterine topography, were foundextending through the umbilical and hypogastric regions. No alterations in structure, echogenicity or echotexture of theother organs were observed, including the ovaries and uterus. On exploratory laparotomy, a cystic structure was found,with 2 segments: the larger one was on the left side, attached to the apex of the bladder by its caudal portion; the otherwas on the right side, attached to the spleen by its cranial portion and to the apex of the bladder by its caudal portion inconnection with the left segment. The ovaries, uterus and uterine horns showed no macroscopic alterations. The structurewas removed, and after analysis (macroscopic morphology, wall histopathology and biochemistry of the contained fluid),it was...(AU)

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Background: The urachus is a tubular structure continuous with the urinary bladder and the allantois in foetal mammals.It serves as a communication between these two structures. At birth, it loses its function and undergoes atrophy by fibrousproliferation within the lumen. When this atrophy process fails at some point, congenital anomalies of the urachus occur.These anomalies are rare in animals, and to our knowledge, the urachal cyst has not yet been described in dogs. The presentwork is unique in that it reports a case of this congenital defect in a dog. Our aim is to increase awareness and to discussthe clinical presentation, the imaging techniques used, and the final diagnosis of this anomaly.Case: A 3-year-old bitch Pit Bull was presented for veterinary assistance to investigate recurrent pseudopregnancy and anirregular estrous cycle. The animal presented in good bodily condition, and the white blood count and clinical biochemistrywere normal. At ultrasound, 2 tubular structures, filled by an echogenic fluid mimicking uterine topography, were foundextending through the umbilical and hypogastric regions. No alterations in structure, echogenicity or echotexture of theother organs were observed, including the ovaries and uterus. On exploratory laparotomy, a cystic structure was found,with 2 segments: the larger one was on the left side, attached to the apex of the bladder by its caudal portion; the otherwas on the right side, attached to the spleen by its cranial portion and to the apex of the bladder by its caudal portion inconnection with the left segment. The ovaries, uterus and uterine horns showed no macroscopic alterations. The structurewas removed, and after analysis (macroscopic morphology, wall histopathology and biochemistry of the contained fluid),it was...

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Feline chronic gingivostomatitis (FCGS) is an oral inflammatory condition that frequently affects felines. Its etiology is not well defined, but several viral agents are thought to be involved. Several therapeutic protocols have been described, yet treatment response is often variable, and the therapeutic success is transient with an unpredictable duration. Therefore, the therapeutic strategy needs to be tailored for each patient. This work relates a case characterized by viral involvement in its etiopathogenesis providing an alternative to the most widely-used methods that so often frustrate both veterinary doctors and pet owners.(AU)

A gengivostomatite crônica felina (FCGS) é uma condição inflamatória oral que frequentemente afeta felinos. A sua etiologia não está bem definida, mas acredita-se que vários agentes virais possam estar envolvidos. Muitos protocolos terapêuticos têm sido descritos, no entanto, a resposta ao tratamento é frequentemente variável e o sucesso terapêutico é transitório com uma duração imprevisível. Portanto, a estratégia terapêutica precisa ser adaptada para cada paciente. O presente trabalho propõe a caracterização do envolvimento viral na etiopatogenia da doença como uma alternativa aos métodos mais amplamente utilizados, que muitas vezes frustram médicos veterinários e os donos de animais de estimação.(AU)

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Feline chronic gingivostomatitis (FCGS) is an oral inflammatory condition that frequently affects felines. Its etiology is not well defined, but several viral agents are thought to be involved. Several therapeutic protocols have been described, yet treatment response is often variable, and the therapeutic success is transient with an unpredictable duration. Therefore, the therapeutic strategy needs to be tailored for each patient. This work relates a case characterized by viral involvement in its etiopathogenesis providing an alternative to the most widely-used methods that so often frustrate both veterinary doctors and pet owners.(AU)

A gengivostomatite crônica felina (FCGS) é uma condição inflamatória oral que frequentemente afeta felinos. A sua etiologia não está bem definida, mas acredita-se que vários agentes virais possam estar envolvidos. Muitos protocolos terapêuticos têm sido descritos, no entanto, a resposta ao tratamento é frequentemente variável e o sucesso terapêutico é transitório com uma duração imprevisível. Portanto, a estratégia terapêutica precisa ser adaptada para cada paciente. O presente trabalho propõe a caracterização do envolvimento viral na etiopatogenia da doença como uma alternativa aos métodos mais amplamente utilizados, que muitas vezes frustram médicos veterinários e os donos de animais de estimação.(AU)

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Background: This work reports a case of urethral obstruction by urolith in a female cat. The patient had a history of hematuria beginning approximately 2 months before visiting the clinic, with anorexia, apathy, emesis, tenesmus and absence of urination. Following physical and ultrasonic examinations, urethral obstruction by urolith was diagnosed. Although feline lower urinary tract disease (FLUTD) is common in both males and females, a urethral obstruction in female cats is extremely rare. This report aims to alert veterinarians to such an occurrence, highlighting the importance of early diagnosis and treatment in view of the severity of the consequences of a urethral obstruction.Case: An undefined, female cat of indefinite age, with a history of hematuria, emaciation, anorexia, apathy, emesis, tenesmus and anuria, presented at our clinic. Physical examination revealed dehydration, discrete rectal prolapse and dysuria. During abdominal palpation, a rigid spherical-shaped structure was observed in the hypogastric region, and complementary examinations were subsequently performed. Hematological examination revealed leukocytosis and increased serum urea and creatinine levels. Abdominal ultrasound examination showed that the urinary vesicle was full, with heterogeneous anechoic content with discrete sedimentation, as well as the presence of a hyperechogenic structure that formed an acoustic shadow at the transition between the bladder trigone and the urethra. In addition, both renal pelves presented dilatation by anechoic content, and measured about 0.5 cm. Treatment with amoxicillin and potassium clavulanate (20 mg/kg administered intravenously) was initiated, and the patient was referred to undergo a surgical procedure to remove the calculus. When exploring the abdominal cavity, the urinary vesicle was identified, which appeared to be rigid.[...](AU)

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Background: This work reports a case of urethral obstruction by urolith in a female cat. The patient had a history of hematuria beginning approximately 2 months before visiting the clinic, with anorexia, apathy, emesis, tenesmus and absence of urination. Following physical and ultrasonic examinations, urethral obstruction by urolith was diagnosed. Although feline lower urinary tract disease (FLUTD) is common in both males and females, a urethral obstruction in female cats is extremely rare. This report aims to alert veterinarians to such an occurrence, highlighting the importance of early diagnosis and treatment in view of the severity of the consequences of a urethral obstruction.Case: An undefined, female cat of indefinite age, with a history of hematuria, emaciation, anorexia, apathy, emesis, tenesmus and anuria, presented at our clinic. Physical examination revealed dehydration, discrete rectal prolapse and dysuria. During abdominal palpation, a rigid spherical-shaped structure was observed in the hypogastric region, and complementary examinations were subsequently performed. Hematological examination revealed leukocytosis and increased serum urea and creatinine levels. Abdominal ultrasound examination showed that the urinary vesicle was full, with heterogeneous anechoic content with discrete sedimentation, as well as the presence of a hyperechogenic structure that formed an acoustic shadow at the transition between the bladder trigone and the urethra. In addition, both renal pelves presented dilatation by anechoic content, and measured about 0.5 cm. Treatment with amoxicillin and potassium clavulanate (20 mg/kg administered intravenously) was initiated, and the patient was referred to undergo a surgical procedure to remove the calculus. When exploring the abdominal cavity, the urinary vesicle was identified, which appeared to be rigid.[...]

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