RESUMEN

The aim was to investigate the potential of germinated soybean proteins asa source of peptides with anticancer and anti-inflammatory activities produced after simulated gastrointestinal digestion. Protein concentrate from germinated soybean was hydrolysed with pepsin/pancreatin and fractionated by ultrafiltration. Whole digest and fractions>10, 5-10, and<5kDa caused cytotoxicity to Caco-2, HT-29, HCT-116 human colon cancer cells, and reduced inflammatory response caused by lipopolysaccharide in macrophages RAW 264.7. Antiproliferative and anti-inflammatory effects were generally higher in 5-10kDa fractions. This fraction was further purified by semi-preparative chromatography and characterised by HPLC-MS/MS. The most potent fraction was mainly composed of ß-conglycinin and glycinin fragments rich in glutamine. This is the first report on the anti-cancer and anti-inflammatory effects of newly isolated and identified peptides from germinated soybean released during gastrointestinal digestion. These findings highlight the potential of germination as a process to obtain functional foods or nutraceuticals for colon cancer prevention.

Asunto(s)

Neoplasias del Colon/metabolismo , Glycine max/metabolismo , Péptidos/metabolismo , Proteínas de Soja/metabolismo , Animales , Antiinflamatorios/química , Antiinflamatorios/metabolismo , Células CACO-2 , Línea Celular Tumoral , Proliferación Celular , Cromatografía Líquida de Alta Presión , Neoplasias del Colon/genética , Neoplasias del Colon/inmunología , Neoplasias del Colon/fisiopatología , Digestión , Germinación/efectos de los fármacos , Humanos , Hidrólisis , Ratones , Pancreatina/química , Pancreatina/metabolismo , Pepsina A/química , Pepsina A/metabolismo , Péptidos/química , Células RAW 264.7 , Proteínas de Soja/química , Glycine max/química , Glycine max/crecimiento & desarrollo , Espectrometría de Masas en Tándem