RESUMEN
Objective To study the effect of simvastatin on high glucose induced vascular endothelial cells injury and explore its action mechanism .Methods Endothelial cells were devided into four group:control group(A) ,high glucose(33 .3 mmol/L)group (B) ,high glucose(33 .3 mmol/L)+simvastatin(1 .0 μmol/L)group(C) ,and high glucose(33 .3 mmol/L)+simvastatin(10 .0 μmol/L)group(D) .The inhibition of cells was detected by CCK-8 assay .The apoptosis was determined by TUNEL assay .The protein ex-pression was detected by Western blot analysis .Results The cells survival rates were (42 .5 ± 6 .4% ) ,(58 .6 ± 7 .8% ) ,and (71 .3 ± 11 .7% ) in B ,C ,and D group respectively .There was significant difference among the three groups(P<0 .05) .The per-cents of apoptosis were (1 .8 ± 0 .6% ) ,(45 .8 ± 8 .9% ) ,(22 .7 ± 6 .4% ) and (12 .6 ± 4 .2% ) in A ,B ,C and D group ,respectively . There was significant difference among the four groups(P<0 .05) .The protein expression of Bcl-2 was lower in B group(0 .13 ± 0 . 03)than that in A group(1 .02 ± 0 .16) .The Bcl-2 expression was higher in C group(0 .28 ± 0 .04)than that in B group ,and in D group(0 .68 ± 0 .11)than that in C group(P<0 .05 ,P<0 .01) .The gene expression of bax was higher in B group than that in A group .The bax expression was lower in C group than that in B group ,and the bax expression was lower in D group than that in C groups(P<0 .05 ,P<0 .01) .Conclusion Simvastatin can inhibit high glucose induced vascular endothelial cells apoptosis .Upregu-lation of Bcl-2 and downregulation of bax may be its action mechanism .
RESUMEN
Objective To summarize radiofrequency cather ablation (RFCA)of tachycardias with right sid-ed accessory atrioventricular pathway in 7 patients with persistent left superior vena cava (PLSVC). Methods RF-CA for right sided accessory atrioventricular pathway in 7 patients with PLSVC was carried out . Coronary sinus ac-cess was performed through the left subclavian and the left femoral vein respectively. Swartz sheath was used during ablation if neccesary. Results There was no siginificant differences in fluoroscopy time between the left subclavian approach and the left femoral vein approach[(2.8 ± 0.6)min vs (3.2 ± 0.3)min, P>0.05)]. No recurrence of ac-cessory pathway conduction was found during a mean follow-up of (23 ± 11) months. The mean discharging time and fluoroscopy time were (312 ± 67)s and (17 ± 5)min respectively. Conclusions Although RFCA of right sided accessory atrioventricular pathway in patients with PLSVC shows specialities in cather manipulation , mapping and ablation, high successful results can be achieved by experienced physician.