RESUMEN

There has been a significant increase in the use of transgenic plants for the large-scale production of pharmaceuticals and industrial proteins. Here, we report the stable accumulation of seed storage proteins containing disease vaccine peptides in transgenic soybean seeds. To synthesize vaccine peptides in soybean seeds, we used seed storage proteins as a carrier and a soybean breeding line lacking major seed storage proteins as a host. Vaccine peptides were inserted into the flexible disordered regions in the A1aB1b subunit three-dimensional structure. The A1aB1b subunit containing vaccine peptides in the disordered regions were sorted to the protein storage vacuoles where vaccine peptides are partially cleaved by proteases. In contrast, the endoplasmic reticulum (ER)-retention type of the A1aB1b subunit containing vaccine peptides accumulated in compartments that originated from the ER as an intact pro-form. These results indicate that the ER may be an organelle suitable for the stable accumulation of bioactive peptides using seed storage proteins as carriers.

Asunto(s)

Vacunas contra el Alzheimer/biosíntesis , Globulinas/biosíntesis , Glycine max/genética , Péptidos/inmunología , Semillas/genética , Proteínas de Soja/biosíntesis , Enfermedad de Alzheimer/inmunología , Enfermedad de Alzheimer/prevención & control , Vacunas contra el Alzheimer/genética , Vacunas contra el Alzheimer/inmunología , Secuencia de Aminoácidos , Retículo Endoplásmico/metabolismo , Expresión Génica , Globulinas/genética , Humanos , Datos de Secuencia Molecular , Mutagénesis Insercional , Péptidos/genética , Plantas Modificadas Genéticamente , Transporte de Proteínas , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/genética , Semillas/metabolismo , Proteínas de Soja/genética , Glycine max/metabolismo , Vacunas , Vacuolas/metabolismo