RESUMEN

Mast cells (MC) are present in the skin and mucous membranes, lymphoid organs, intestine wall and brain, where they are located close to the blood and lymphatic vessels and nerve terminals. As a source of a large number of biologically active substances, many of which are released quickly into the environment as a result of degranulation process, mast cells play an essential role in the regulation of physiological processes in the tissues where they are present. Changing the MC population and activity in the tissues during aging is associated with age-related changes of the skin and mucous membranes, the development of central nervous system disorders such as itching, headache, joint and muscle pain, memory loss, attention deficits, depression, anxiety and depressive disorders, autism, Alzheimer's disease and multiple sclerosis.

Asunto(s)

RESUMEN

AIM: To study bioenergetic processes in the synovial fluid (SF) in osteoarthrosis and to study relevant effects of chondroitinsulphate (structum). MATERIAL AND METHODS: Changes in bioenergy parameters of SF were analysed in the course of 3-month structum treatment of 15 osteoarthrosis (OA) patients. Classic enzymatic tests and polarographic test of SF cells oxygen absorption rate were used. Physiological-biochemical indices were compared at the height of the disease and after therapy. RESULTS: A significant shift of SF pH to the acid direction was found in OA patients. There was also transformation of bioenergetic processes with resultant loss of synovial cells energetics (low ATP and switch on of a reserve energetic mechanism of creatinephosphate consumption). Pharmacological correction of energy metabolism of SF cells was obtained due to action of chondroprotector structum. CONCLUSION: OA provokes bioenergetic changes in SF with activation of glycolysis, employment of reserve bioenergetic mechanisms, enhancement of creatinephosphate degradation, discoordination of respiration with oxidant phosphorilation. The pH shift to acid direction (from 7.4 to 6.85 in OA) is a trigger mechanism of OA. Replacement therapy with polyanion drug structum corrects pH in 3 months and bioenergetic parameters.

Asunto(s)

RESUMEN

Treatment with roxicam, rengasil and flugalin tended significantly to normalize the activity of enzymes of carbohydrate metabolism pentosophosphate pathway in the blood of patients with systemic lupus erythematosus and rheumatoid arthritis. The dynamics of the change in the activity of enzymes of pentose phosphate pathway in the blood of patients with systemic connective tissue diseases against the background of treatment with roxicam, rengasil and flugalin is the enzymological criterion in assessing efficacy of pharmacological correction of the inflammatory process in the diseases.

Asunto(s)

RESUMEN

Ratios of activities of oxidative and nonoxidative enzymes involved in the pentose phosphate pathway of carbohydrate metabolism were altered in blood plasma and cells of the patients with systemic impairments of connective tissue. In rheumatoid arthritis and systemic lupus erythematosus the enzymatic activity was increased in blood plasma and cells, while the most distinct activation of the enzymes was found in granulocytes. In systemic sclerodermia total activity of the enzymes involved in metabolism of pentose phosphates in granulocytes exceeded 2.6-fold their values under conditions of normal state, whereas activities of the other enzymes studied remained near normal values. Calculation of ratios (mu value) between activities of the pathway oxidative and nonoxidative enzymes showed that the mu value was increased 2.7-fold in rheumatoid arthritis, while this value was decreased in lupus erythematosus and systemic sclerodermia 4.2- and 2-fold, respectively. The mu values, calculated on the basis of estimation of total activity of dehydrogenases from pentose phosphate pathway and total pentose phosphate metabolizing activity, might serve as a convenient diagnostic criterion for estimation of the ratio between activities of oxidative and nonoxidative enzymes involved in pentose phosphate pathway in granulocytes used for differential diagnosis of systemic impairments of connective tissue (rheumatoid arthritis, lupus erythematosus and systemic sclerodermia).

Asunto(s)

Asunto(s)

RESUMEN

A study was made of the content of prostaglandins E, A, F2 alpha and malonic dialdehyde (MDA) in the blood plasma and urine of patients with systemic lupus erythematosus, systemic scleroderma, rheumatoid arthritis and healthy persons. The levels of plasma and urine prostaglandins and MDA were significantly elevated in systemic lupus erythematosus and rheumatoid arthritis, and in systemic scleroderma they did not differ from those in normal. The ratio of pressor and depressor prostaglandins in systemic lupus erythematosus and rheumatoid arthritis did not change, and in systemic scleroderma there was a strong shift to pressor prostaglandins. Change in the content and ratio of prostaglandins and MDA in systemic diseases of connective tissue was suggestive of differences of pathogenetic effects of these mediators in some diseases of this group.

Asunto(s)

RESUMEN

A procedure is developed for estimation of glucose-6-phosphate and 6-phosphogluconate dehydrogenases activity in human erythrocytes. The procedure involved polarographic registration of oxygen consumption, which is directly related to content of NADPH formed, in a coupled enzymatic system containing peroxidase and Mn2+.

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)

Asunto(s)