RESUMEN
Infectious bursal disease (IBD), also known as Gumboro disease, is a globally well-known disease with a significant socio-economic effect. For control of IBD, several commercial egg- and cell-based vaccines are prepared. The cell-based IBD vaccines are significantly cost-effective; however, it is essential to confirm their safety and efficacy. The main cell line used to product the cell-based IBD vaccines, is a primary chicken embryo fibroblast (CEF). Nevertheless, manipulation of CEF is extremely challenging and time-consuming. This study aimed to characterize a sensitive suspension cell culture from ovine lymphoid, according to WHO technical report series; No. 978, Annex III. This authentication covered the growth curves, sensitivity, stability, karyotyping and identifying the adventitious agents. This cell line passed all defined tests and was considered as a suitable one for IBD vaccine preparation in a large scale.
Asunto(s)
Infecciones por Birnaviridae/veterinaria , Pollos , Virus de la Enfermedad Infecciosa de la Bolsa/inmunología , Linfocitos/virología , Enfermedades de las Aves de Corral/prevención & control , Oveja Doméstica , Vacunas Virales/inmunología , Animales , Infecciones por Birnaviridae/prevención & control , Infecciones por Birnaviridae/virología , Línea Celular , Enfermedades de las Aves de Corral/virologíaRESUMEN
OBJECTIVES: Domestic contamination with mold, cockroaches, rodents, and dust worsens asthma severity. This violates warranty of habitability laws in most of the states, but patients often find it beyond their means to remedy their housing situation. We aimed to study the effect of a medical-legal collaborative intervention to force landlords into providing better living conditions for patients with poorly controlled asthma. METHODS: We retrospectively studied charts of adult patients aged 18 years or older with poorly controlled asthma (moderate or severe persistent) despite maximum medical therapy. Additionally, patients had self-reported domestic allergen exposures such as mold, cockroaches, mice or rats, and dust. The patients received legal assistance to improve their domestic environments, including fixing leaks, exterminating pests, or providing a different apartment. Post-intervention change in peak expiratory flow rate (PEFR), asthma severity class, medications, emergency department (ED) visits, hospitalizations, and requirement for systemic steroids for symptom control was assessed. RESULTS: Data were available for 12 patients (9-12 months pre-intervention and 6-12 months post-intervention). Analysis of paired data revealed that mean PEFR rose by 38.6 LPM (95% CI: 9.9-67.3; p = .014). The number of ED visits and hospital admissions declined from 22 ED visits and 11 admissions to 2 ED visits and 1 admission (91% reduction), respectively. Of the 11 patients requiring systemic steroids, only three required these post-intervention. All patients had reductions in the dose and/or number of medications. During post-intervention, 11 (91.7%) patients dropped ≥2 classes in asthma severity. CONCLUSIONS: Medical-legal collaboration is highly effective in improving the control of inner-city asthmatics by effecting improvements in the domestic environment.
Asunto(s)
Asma/terapia , Ambiente , Exposición a Riesgos Ambientales/legislación & jurisprudencia , Exposición a Riesgos Ambientales/prevención & control , Vivienda/legislación & jurisprudencia , Población Urbana , Adulto , Anciano , Anciano de 80 o más Años , Animales , Antiasmáticos/administración & dosificación , Asma/epidemiología , Cucarachas , Polvo , Servicio de Urgencia en Hospital/estadística & datos numéricos , Exposición a Riesgos Ambientales/estadística & datos numéricos , Femenino , Hongos , Hospitalización/estadística & datos numéricos , Humanos , Masculino , Ratones , Persona de Mediana Edad , Ápice del Flujo Espiratorio , Ratas , Índice de Severidad de la Enfermedad , Factores SocioeconómicosRESUMEN
L'otite externe necrosante est une pathologie grave qui pose de plus en plus de difficultes dans la prise en charge therapeutique. Nous rapportons dans ce travail une observation d'otite externe necrosante compliquee d'une paralysie faciale qui avait resiste au traitement medical et chirurgical bien conduits. L'adjonction de l'oxygenotherapie hyperbare comme traitement complementaire a permis une regression des signes cliniques et une guerison de l'infection. Il s'agissait du premier cas d'otite externe necrosante ayant beneficie en mars 1996 de l'oxygenotherapie hyperbare en Tunisie. A travers cette observation; nous discutons l'indication de l'oxygenotherapie hyperbare dans le traitement de l'otite externe necrosante