RESUMEN

With the rapid increase in dementia in developed countries, it is important to establish methods for maintaining or improving cognitive function in elderly people. To resolve such problems, we have been developing a cosmetic therapy (CT) program for elderly women. However, the mechanism and limitations of CT are not yet clear. In order to clarify these issues, we employed time-resolved spectroscopy (TRS) to evaluate the effect of CT on prefrontal cortex (PFC) activity in elderly females with various levels of cognitive impairment. Based on the Mini-Mental State Examination (MMSE) score, the subjects were classified into mild (mean MMSE score: 24.1±3.8) and moderate (mean MMSE score: 10.3±5.8) cognitive impairment (CI) groups (p<0.0001). The mild CI group exhibited significantly larger baseline concentrations of oxy-Hb and t-Hb than the moderate CI group. CT significantly increased the baseline concentrations of oxy-Hb (p<0.002) and t-Hb (p<0.0013) in the left PFC in the mild CI group. In contrast, CT did not change the concentrations of oxy-Hb and t-Hb in the moderate CI group (p>0.05). These results suggest that CT affects cognitive function by altering PFC activity in elderly women with mild CI, but not moderate CI.

Asunto(s)

Cognición , Cosméticos , Anciano , Femenino , Humanos , Corteza Prefrontal/fisiología , Análisis Espectral

RESUMEN

INTRODUCTION: We report a case of low-risk stomach gastrointestinal stromal tumor (GIST) which has been under a long-term observation, obtaining from this experience knowledge useful in determining the treatment formula for this disease. RESULTS: During the observation for 6 years, no such change as ulcer formation was observed in the appearance of the tumor. The measurement of tumor diameter, however, showed gradual growth of maximum tumor diameter from 2.7 to 5.0 cm. When the changes in the diameter of tumor during this period is plotted, taking the timeon the horizontal axis and the tumor diameter on the vertical axis, the growth of the tumor can be approximated with a secondary function, making it possible to estimate the developmental period of the GIST concerned from the approximated secondary function. Thus, the developmental period in this case was estimated to go back 19 years before the time when it was discovered for the 1(st) time. Further, it was considered that the coefficient of the secondary function represents the rate of tumor growth, and that comparison with this coefficient contributed to the evaluation of malignancy stage of the GIST concerned. CONCLUSION: The growth curve predicting the growth of tumor could be depicted by measuring the diameter of the tumor in GIST twice or more at an interval of 6-12 months with EUS, and it was thought that this was utilizable for determining treatment formula for GISTs.

RESUMEN

Aberrant pancreas is used to describe ectopic pancreatic tissue lying outside its normal location with no anatomic or vascular connection to the pancreas proper. Patients with aberrant pancreas are usually asymptomatic, so aberrant pancreas are typically discovered incidentally during endoscopy, surgery, or autopsy. This time, we report a case of gastric aberrant pancreas bleeding was repeated and endoscopic hemostasis was difficult. A 22-year-old man was admitted to a hospital with a complaint of epigastric pain and melena. Upper gastrointestinal endoscopy and endoscopic ultrasonography (EUS) revealed a submucosal tumor with a bleeding ulcer at the anterior wall of the antrum in the stomach, and diagnosed it as an aberrant pancreas. It was hard to stop bleeding by in total 7 times endoscopic hemostasis and anemia was gradually progressed, so partial gastrectomy was performed. This gastric tumor measured 40 mm × 30 mm × 20 mm and had a severe ulcerative change. The pathological diagnosis was aberrant pancreas with Langerhans islet, acinous cells and excretory duct. (Heinrich type) Until December 2013 in Japan, 13 cases of gastric aberrant pancreas with bleeding have been reported and in these, a surgery was done in 11 cases. In gastric aberrant pancreas cases with ulcer formation like this case, endoscopic hemostasis is expected to be difficult, and surgery is necessary. Hence, early accurate diagnosis by EUS is a very important to decide better treatment plan.

RESUMEN

The synthesis of cyclic ADP-carbocyclic-ribose (2), as a stable mimic for cyclic ADP-ribose, was investigated. Construction of the 18-membered backbone structure was successfully achieved by condensation of the two phosphate groups of 19, possibly due to restriction of the conformation of the substrate in a syn-form using an 8-chloro substituent at the adenine moiety. SN2 reactions between an optically active carbocyclic unit 8, which was constructed by a previously developed method, and 8-bromo-N6-trichloroacetyl-2',3'-O-isopropylideneadenosine 9c gave N-1-carbocyclic derivative, which was deprotected to give 5'-5"-diol derivatives 18. When 18 was treated with POCl3 in PO(OEt)3, the bromo group at the 8-position was replaced to give N-1-carbocyclic-8-chloroadenosine 5',5"-diphosphate derivative 19 in 43% yield. Treatment of 19 with 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride gave the desired intramolecular condensation product 20 in 10% yield. This is the first chemical construction of the 18-membered backbone structure containing an intramolecular pyrophosphate linkage of a cADPR-related compound with an adenine base.

Asunto(s)

Adenina/química , Adenosina Difosfato Ribosa/análogos & derivados , Halógenos/química , Adenosina Difosfato Ribosa/síntesis química , Adenosina Difosfato Ribosa/química , ADP-Ribosa Cíclica , Difosfatos/química , Espectroscopía de Resonancia Magnética

RESUMEN

PURPOSE: The purpose of this study are as follows: 1. To determine incidence proportion estimations and prevalence of pressure ulcers in home care settings. 2. To clarify the effective nursing care for prevention and treatments of pressure ulcers at visiting nursing service stations. 3. To show whether demographic characteristics are factors in pressure ulcer development. METHODS: This research is a part of the series of studies on prevalence of pressure ulcers in 934 visiting nursing service stations on October 1995 in Japan. This was followed in November 1, 1996 to October 31, 1997, by another investigation for incidence proportion estimation and prevalence of pressure ulcers and their relationship to nursing care facilities, in which questionnaires were mailed to 620 facilities that participated in the first research. Responses from 294 (47.4%) facilities and 1,208 subjects were received. We analyzed the responses from 215 facilities (73.1%) and 864 subjects to the two types of questionnaires. RESULTS: Prevalence of pressure ulcers was found to be 8.0% (95% CI: 7.5-8.6) on July 1, 1997 and incidence proportion estimation was 3.7% (95% CI: 3.3-4.1) from November 1, 1996 to October 31, 1997 for the 10,750 clients in 215 facilities. The number of prescriptions for turning of client sleep position (p = 0.044), and having a standard care assessment procedure (p = 0.015) were significantly higher for no-present pressure ulcer than in facilities with under 5% having present pressure ulcers and facilities with over 5% having present pressure ulcers. Subtracting the prevalence point in 1997 from 1995, the utilization of a primary nursing care plan (p = 0.021), and the number of part-time nurses (p = 0.008) were significantly higher in the groups that had a decrease or no-change compared to the increased groups. 45.6% of the ulcers identified were situated on the sacrum/buttocks and 39.4% of the ulcers were grade 2. The mean total score on the Braden Scale was 12.7. A score of 18 may be considered as the cut-off point in home care settings. DISCUSSION: Prevalence of pressure ulcers was found to be 8.0% and incidence proportion estimation was 3.7%. The present findings concerning incidence proportion estimation and prevalence of pressure ulcers and the practice of nursing care in visiting nursing service stations suggests possibility for improving outcomes for clients with pressure ulcers and for those at risk factors of developing them.

Asunto(s)

Servicios de Atención de Salud a Domicilio , Úlcera por Presión/epidemiología , Úlcera por Presión/terapia , Anciano , Anciano de 80 o más Años , Femenino , Servicios de Atención de Salud a Domicilio/estadística & datos numéricos , Humanos , Japón/epidemiología , Masculino , Prevalencia , Encuestas y Cuestionarios

RESUMEN

We performed transcatheter thrombolysis on a 64-year-old man with non-occluding superior mesenteric artery (SMA) thrombosis because his severe symptoms could not be controlled with medication. An enhanced computed tomography (CT) scan revealed intramural thrombosis in the SMA. We were concerned that the narrowing of the SMA lumen might progress to complete occlusion, resulting in a high likelihood of mortality. After dissolution of the SMA thrombosis, the original symptoms almost completely disappeared. However, intracranial hemorrhage occurred 8 hr after thrombolysis, requiring surgical intervention. Transcatheter thrombolysis is thought to be a useful treatment for SMA thrombosis, especially in elderly patients with a high operative risk; however, the possibility of intracerebral hemorrhage must be taken into consideration.

Asunto(s)

Cateterismo Venoso Central/efectos adversos , Hemorragia Cerebral/etiología , Arterias Mesentéricas , Trombosis/terapia , Angiografía , Cateterismo Venoso Central/instrumentación , Hemorragia Cerebral/diagnóstico , Hemorragia Cerebral/cirugía , Craneotomía , Estudios de Seguimiento , Humanos , Masculino , Persona de Mediana Edad , Terapia Trombolítica/efectos adversos , Trombosis/diagnóstico , Tomografía Computarizada por Rayos X , Resultado del Tratamiento

RESUMEN

1. Endogenous nitric oxide (NO) can be detected in exhaled air and accumulates in inflamed airways. However its physiological role has not been fully elucidated. In this study, we investigated a role for endogenous NO in allergen-induced airway responses. Sensitised guinea-pigs were treated with NG-nitro-L-arginine methyl ester L-NAME (2.0 mM) or aminoguanidine (AG) (2.0 mM) 30 min before the allergen challenge, and 3 and 4 h after the challenge. Alternatively, L-arginine (2.4 mM) treatment was performed 30 min before, and 2 and 3 h after the challenge. In all groups, ovalbumin (OVA) challenge (2 mg ml(-1) for 2 min) was performed, and airway responses, NO production, infiltration of inflammatory cells, plasma exudation and histological details were examined. 2. Allergen-challenged animals showed an immediate airway response (IAR) and a late airway response (LAR), which synchronised with an increase in exhaled NO. Treatment with L-NAME and AG did not affect IAR while they significantly blocked LAR (72% and 80% inhibition compared to vehicle) and production of NO (35% and 40% inhibition). On the other hand, treatment with L-arginine did not affect IAR but potentiated LAR (74% augmentation). 3. In bronchoalveolar lavage (BAL) fluid, allergen-induced increases in eosinophils were reduced by 48% for L-NAME treatment compared to vehicle, and increased by 56% for L-arginine treatment. 4. Treatment with L-NAME significantly decreased airway microvascular permeability to both Monastral blue (MB) and Evans blue (EB) dye (50.6% and 44% inhibition). 5. We conclude that allergen-induced LAR is closely associated with NO production, and that NO plays a critical role in inflammatory cell infiltration and plasma exudation in the allergic condition.

Asunto(s)

Alérgenos/inmunología , Bronquios/inmunología , Hiperreactividad Bronquial/inmunología , Óxido Nítrico/fisiología , Tráquea/inmunología , Animales , Bronquios/fisiopatología , Líquido del Lavado Bronquioalveolar , Inhibidores Enzimáticos/farmacología , Extravasación de Materiales Terapéuticos y Diagnósticos , Femenino , Guanidinas/farmacología , Cobayas , Microscopía Electrónica , NG-Nitroarginina Metil Éster/farmacología , Óxido Nítrico Sintasa/antagonistas & inhibidores , Tráquea/fisiopatología , Tráquea/ultraestructura

RESUMEN

We have isolated a full-length cDNA for an inducible nitric oxide synthase (iNOS) from guinea-pig lung. The cDNA has a 3447 bp open reading frame encoding 1149 amino acid residues. The deduced amino acid sequence is approx. 80% identical with iNOS of human epithelial cells and murine macrophages. Consensus recognition sites for cofactors are highly conserved. COS cell lysate transfected with the guinea-pig iNOS shows significant levels of nitric oxide synthase (NOS) activity, and this is inhibited by 79% by chelation of Ca2+ ions. The NOS activity is restored in a concentration-dependent manner by increasing the free Ca2+ level. The NOS activity is also inhibited by trifluoperazine, a calmodulin antagonist, which suggests that the Ca2+ dependence is due to Ca2+-dependent calmodulin binding to the enzyme. Northern blot analysis reveals that the cloned iNOS mRNA is expressed in the lung and the colon in normal guinea pigs. Stimulation in vivo by lipopolysaccharide induces the expression of iNOS in the kidney, the spleen and the colon, but in the lung the same stimulation decreases its expression. These results suggest that the cloned guinea-pig iNOS is distinct in characteristics and expression from previously described iNOS forms.

Asunto(s)

Calcio/metabolismo , Pulmón/enzimología , Óxido Nítrico Sintasa/genética , Óxido Nítrico Sintasa/metabolismo , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Northern Blotting , Células COS/enzimología , Clonación Molecular , ADN Complementario/genética , ADN Complementario/metabolismo , Cobayas , Humanos , Ratones , Datos de Secuencia Molecular , Óxido Nítrico Sintasa de Tipo II , Proteínas Recombinantes/biosíntesis , Homología de Secuencia de Aminoácido

RESUMEN

So-called "negative health behaviors" (e.g. smoking, excessive eating/drinking), which are related to morbidity and mortality, are generally well known to the public. However there are many who undertake negative health behaviors despite knowing of their negative health effects. Using a mail survey of subjects aged 25 to 54, we examined reasons and justifications for engaging in negative health behaviors and their related factors from the lay person's perspective. Concerning the reasons for negative health behaviors, we hypothesized seven concepts (work priority, group harmony priority, the contribution to relaxation, tools for communication, prior orientation focused on the present, individualistic priority, and appearance), and devised multiple questions for each concept. The results are as follows. 1. Compared to females, males tended to support of the five concepts about the internalized norms concerned with negative health behaviors (e.g. the tendency of each individual to approve or disapprove of the reasons for negative health behaviors). In females, the younger generation and those that were married expressed more support than the older and the unmarried. There were differences in males according to occupation. 2. Concerning which reference groups had the largest influence on the social norms concerned with negative health behaviors (e.g. the perceptions as to what degree a group regards the reasons about negative health behaviors as desirable), most perceived that the "office" and "friends" groups would be more supportive of reasons for negative health behaviors than the "family" groups. 3. The health behaviors, which correlated with the internalized norms or the social norms concerned with negative health behaviors, were eating breakfast, smoking, and drinking (frequency and amount). In particular, drinking behavior was influenced most by the internalized norms and the social norms.

Asunto(s)

Actitud Frente a la Salud , Conductas Relacionadas con la Salud , Conformidad Social , Adulto , Femenino , Humanos , Masculino , Persona de Mediana Edad

RESUMEN

The C-C chemokine eotaxin is thought to be important in the selective recruitment of eosinophils to the site of inflammation in guinea pigs, mice, and humans. We isolated the rat eotaxin gene to determine whether a similar molecule might play a role in the pulmonary infiltration of eosinophils during acute inflammation in the rat. The cDNA for rat eotaxin encoded a 97-amino acid protein containing a 74-amino acid mature eotaxin protein with 97.3% identity to mouse eotaxin. The recombinant protein encoded by this gene displayed specific chemotactic activity for eosinophils when analyzed with a microchemotactic chamber. The expression of eotaxin mRNA increased approximately 1.6-fold immediately after exposure to ozone and was 4-fold higher after 20 h. The number of lavageable eosinophils at the same time points were 3- and 15-fold greater, respectively, than control eosinophils. Immunocytochemistry revealed that alveolar macrophages and bronchial epithelial cells were positive for eotaxin. These results suggest that eotaxin may be involved in the recruitment of eosinophils into the air spaces during certain inflammatory conditions in rats.

Asunto(s)

Quimiocinas CC , Citocinas/biosíntesis , Regulación de la Expresión Génica/efectos de los fármacos , Pulmón/metabolismo , Ozono/toxicidad , Administración por Inhalación , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Quimiocina CCL11 , Factores Quimiotácticos Eosinófilos/biosíntesis , Quimiotaxis de Leucocito , Clonación de Organismos , Citocinas/química , Granulocitos/efectos de los fármacos , Granulocitos/fisiología , Cobayas , Humanos , Pulmón/efectos de los fármacos , Pulmón/patología , Masculino , Ratones , Datos de Secuencia Molecular , Ozono/administración & dosificación , Reacción en Cadena de la Polimerasa , Biosíntesis de Proteínas/efectos de los fármacos , ARN Mensajero/biosíntesis , Ratas , Ratas Endogámicas BN , Proteínas Recombinantes/biosíntesis , Proteínas Recombinantes/química , Alineación de Secuencia , Homología de Secuencia de Aminoácido , Transcripción Genética/efectos de los fármacos

RESUMEN

A murine monoclonal antibody MLS102 recognizes sialosyl-Tn antigen in mucin and immunohistochemically reacts with more than 80% of colorectal cancer tissues. The purpose of this study was to assess the usefulness of this monoclonal antibody for the immunoscintigraphy of colorectal cancer. Planar and SPECT images were obtained on day 2 or day 3 after injection of 2 mg and 74 MBq 111In-labeled MLS102 antibody into 17 patients with colorectal cancer. Nine of 11 primary tumors and 4 of 6 locally recurrent tumors were detected. Positive images were obtained in all tumors larger than 4.5 x 2.7 cm. Three tumors of less than 2.5 cm and 1 recurrent tumor, which was missed by other imaging modalities, were negative. There were no adverse reactions. Human anti-(mouse Ig) antibody developed in 4 patients. Although improvement of detectability for smaller tumors needs to be pursued, the antibody MLS102 is potentially promising for use in immunoscintigraphy of colorectal cancer.

Asunto(s)

Neoplasias del Colon/diagnóstico por imagen , Radioisótopos de Indio , Mucinas/inmunología , Radioinmunodetección/métodos , Neoplasias del Recto/diagnóstico por imagen , Adulto , Anciano , Anciano de 80 o más Años , Anticuerpos Monoclonales , Antígenos de Carbohidratos Asociados a Tumores/análisis , Neoplasias del Colon/metabolismo , Femenino , Humanos , Radioisótopos de Indio/farmacocinética , Masculino , Persona de Mediana Edad , Recurrencia Local de Neoplasia/diagnóstico por imagen , Neoplasias del Recto/metabolismo , Tomografía Computarizada de Emisión de Fotón Único

RESUMEN

Cyclic ADP-ribose (cADPR, 1) is a newly discovered general mediator involved in Ca2+ signaling. Carbocyclic analogues of 1, namely, 2 and 3, were designed as a stable isosteres of 1, and their synthesis was studied. The reaction of a triflate 9 which was derived from known diol 4, and inosine derivative 10 gave desired N1-alkyl derivative 11 in 38% yield, and then it was converted to diphosphate derivative 12.

Asunto(s)

Adenosina Difosfato Ribosa/análogos & derivados , Adenosina Difosfato Ribosa/síntesis química , Adenosina Difosfato Ribosa/química , Adenosina Difosfato Ribosa/metabolismo , Calcio/metabolismo , ADP-Ribosa Cíclica , Líquido Intracelular , Métodos , Conformación Molecular , Estructura Molecular , Sistemas de Mensajero Secundario

RESUMEN

Arterial chemoembolization was performed three times preoperatively for cancer associated with fistula-in-ano for diminishing the postoperative surgical tissue defect of the perineum. Partial response of the tumor to this treatment was confirmed by computed tomography. A sixty-two-year-old male patient recognized a mass in the perineum one and a half years ago. He had suffered from fistula-in-ano for thirty years. The mass increased its size and formed a tumor in the bilateral buttocks (12 x 9 cm and 9 x 9 cm in diameter). Biopsy specimen showed a moderately differentiated adenocarcinoma. Angiography showed a hypervascular tumor stain. The main feeders to the tumor were the superior rectal artery and bilateral internal iliac artery. Arterial chemoembolization was performed three times through the right and left internal iliac artery with Gelfoam particle. Arterial infusion chemotherapy was added through inferior mesenteric artery and bilateral internal iliac artery with a total 120 mg of adriamycin and 1,500 mg of 5-FU simultaneously. The tumor decreased in size to 44% of the preoperative size by the treatment. A radical operation was performed following the preoperative treatment. Preoperative arterial chemoembolization for cancer associated fistula-in-ano was considered to be helpful for local control.

Asunto(s)

Adenocarcinoma/terapia , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Neoplasias del Ano/terapia , Quimioembolización Terapéutica , Fístula Rectal/complicaciones , Adenocarcinoma/complicaciones , Adenocarcinoma/cirugía , Neoplasias del Ano/complicaciones , Neoplasias del Ano/cirugía , Quimioterapia Adyuvante , Doxorrubicina/administración & dosificación , Fluorouracilo/administración & dosificación , Esponja de Gelatina Absorbible/administración & dosificación , Humanos , Infusiones Intraarteriales , Masculino , Persona de Mediana Edad

RESUMEN

The new immunoradiometric assay for CA125 (CA125II assay) uses the monoclonal antibody M11 as an immunoadsorbent. The epitope recognized by M11 is different from the OC125 epitope. Monoclonal antibodies 130-22 and 145-9 recognize an epitope designated as CA130 on the molecule expressing the OC125 epitope. Similarity of M11 epitope to the epitope of anti-CA130 antibodies and dissociation of antigen levels measured by the original CA125 assay and new CA125II assay were examined. Anti-CA130 antibodies partially competed with M11 for the M11 epitope. Among more than 20,000 serum samples we found 12 patients in whom the serum CA125 concentration measured by the CA125II assay was different from that measured by the original assay. In 11 out of 12 patients the CA125 concentration was moderately or extremely high by the original assay but very low by the CA125II assay. Eight of the 11 patients had benign disease, one had no apparent disease and two had cancer. The antigen level determined by CA130 assay was very low in all the 11 patients. In one patient the CA125II assay showed a higher antigen level than the original assay or CA130 assay. The heterogeneity of the epitope expression could cause the dissociation of CA125 levels measured by the different monoclonal antibodies.

Asunto(s)

Anticuerpos Monoclonales/inmunología , Antígenos de Carbohidratos Asociados a Tumores/sangre , Adulto , Anciano , Animales , Antígenos de Carbohidratos Asociados a Tumores/inmunología , Epítopos , Femenino , Humanos , Ratones , Persona de Mediana Edad

RESUMEN

UNLABELLED: Human small-cell lung cancer (SCLC) is considered a feasible target for immunotherapy using a radiolabeled monoclonal antibody (Mab). A murine Mab, NE150 (IgG1), reacts with the neural cell adhesion molecule, which is identical to cluster 1 antigen of SCLC. METHODS: To estimate their therapeutic effects, NE150 and an isotype-matched control Mab were labeled with 131I and administered intravenously as a single dose into athymic mice inoculated with a NCI-H69 SCLC xenograft. The absorbed dose in organs was also examined based upon a long-term biodistribution study of 131I-NE150. RESULTS: Tumors (initial volume 563.4 +/- 223.5 mm3) treated with 11.1 MBq (300 microCi) of 131I-NE150 diminished and became invisible at days 30-33, demonstrating a 60-day mean growth delay to reach a tripled initial volume compared with sham-treated tumors. Cumulative absorbed doses were estimated to be 2310, 410, 500, 330, and 790 cGy for the tumor, liver, kidney, spleen and lung, respectively. CONCLUSION: Iodine-131-NE150 had potent therapeutic effects against SCLC transplants in athymic mice, however, careful assessment of the side effects, improvement of radioiodination and chimerization of the Mab might be necessary to achieve efficient targeting in clinical therapeutic applications.

Asunto(s)

Anticuerpos Monoclonales/uso terapéutico , Carcinoma de Células Pequeñas/radioterapia , Neoplasias Pulmonares/radioterapia , Radioinmunoterapia , Trasplante Heterólogo , Carcinoma de Células Pequeñas/inmunología , Moléculas de Adhesión Celular Neuronal/inmunología , Moléculas de Adhesión Celular Neuronal/metabolismo , Humanos , Neoplasias Pulmonares/inmunología , Distribución Tisular

Asunto(s)

Neoplasias Encefálicas/diagnóstico por imagen , Neoplasias Encefálicas/secundario , Carcinoma de Células Renales/diagnóstico por imagen , Carcinoma de Células Renales/secundario , Compuestos de Organotecnecio , Cuero Cabelludo , Neoplasias Cutáneas/diagnóstico por imagen , Neoplasias Cutáneas/secundario , Succímero , Humanos , Neoplasias Renales/patología , Masculino , Persona de Mediana Edad , Ácido Dimercaptosuccínico de Tecnecio Tc 99m , Tomografía Computarizada de Emisión de Fotón Único

RESUMEN

Radiolabeled GA-17, a murine monoclonal antibody that reacts specifically with glioma cells, bound to a small-cell lung cancer (SCLC) cell line NCI-H69 derived from neural cells, both in vitro and in vivo. The affinity constant of GA-17 F (ab')2 fragment binding to NCI-H69 was 1.02 x 10(8)/M while that to the glioma cell line U87MG was 1.22 x 10(8)/M. Iodine-125-labeled GA-17 F(ab')2 fragments injected i.v. localized well in NCI-H69 cells xenografted in nude mice. The percentage of the injected dose per gram accumulated in the xenografted tumor was 6.87 +/- 1.34% g-1 (mean +/- SD, n = 5) 24 h after injection. On the other hand, control monoclonal F(ab')2 fragments accumulated in the xenografted tumor at 0.75 +/- 0.30% g-1. The tumor-to-blood ratio was 1.8 for NCI-H69, while that of control F(ab')2 was 0.60. In conclusion, the radiolabeled GA-17 F(ab')2 fragment is expected to be useful clinically to visualize the small-cell lung cancer and in radioimmunotherapy.

Asunto(s)

Anticuerpos Monoclonales/inmunología , Carcinoma de Células Pequeñas/diagnóstico por imagen , Glioma/inmunología , Inmunotoxinas , Neoplasias Pulmonares/diagnóstico por imagen , Animales , Anticuerpos Monoclonales/metabolismo , Especificidad de Anticuerpos , Carcinoma de Células Pequeñas/inmunología , Femenino , Humanos , Fragmentos de Inmunoglobulinas/inmunología , Fragmentos de Inmunoglobulinas/metabolismo , Inmunotoxinas/metabolismo , Radioisótopos de Yodo , Neoplasias Pulmonares/inmunología , Ratones , Ratones Endogámicos BALB C , Ratones Desnudos , Trasplante de Neoplasias , Cintigrafía , Distribución Tisular , Trasplante Heterólogo

RESUMEN

PURPOSE: To assess the potential of technetium-99m (V) dimercaptosuccinic acid (DMSA) scintigraphy for diagnosis of soft-tissue tumors. MATERIALS AND METHODS: Tc-99m (V) DMSA scintigraphy was performed in 76 patients with histologic proof of soft-tissue tumors. In 57 of these patients, gallium-67 citrate scintigraphy was performed within 2 weeks after Tc-99m (V) DMSA scintigraphy. RESULTS: Uptake of Tc-99m (V) DMSA was clearly detected in almost all sarcomas, metastatic carcinomas, highly recurrent benign tumors (ie, extra-abdominal desmoids and tenosynovial giant cell tumors), superficial and deep hemangiomas, and granulomatous soft-tissue lesions but was not found in other benign solid tumors of soft tissue. Uptake of Ga-67 citrate was clearly detected in all inflammatory lesions and metastatic carcinomas, eight of 14 sarcomas (57%), and two of 10 highly recurrent benign tumors (20%) but was not found in hemangiomas or benign soft-tissue tumors. CONCLUSION: Low-grade malignant and highly recurrent benign lesions, which often recur after surgery, were detected more accurately with Tc-99m (V) DMSA scintigraphy than with Ga-67 citrate scintigraphy.

Asunto(s)

Compuestos de Organotecnecio , Neoplasias de los Tejidos Blandos/diagnóstico por imagen , Succímero , Adolescente , Adulto , Anciano , Niño , Citratos , Ácido Cítrico , Femenino , Radioisótopos de Galio , Humanos , Masculino , Persona de Mediana Edad , Cintigrafía , Sensibilidad y Especificidad , Neoplasias de los Tejidos Blandos/patología , Ácido Dimercaptosuccínico de Tecnecio Tc 99m

RESUMEN

We evaluated "BALL ELSA CYFRA21-1" kit, an immunoradiometric assay kit for cytokeratin 19. Monoclonal antibodies KS19-1 and BM19-21 are used for immunoadsorbent and indicator, respectively. There was no problems in reproducibility, dilution test and recovery test. Minimum detectable concentration was 0.42 ng/ml. The antigen measured by this kit was immunologically cross-reactive with tissue polypeptide antigen (TPA) and CYFRA21-1 concentration was closely correlated with TPA concentration in patient's serum. One of twenty-six healthy subjects showed serum concentration over a cut-off value of 2.0 ng/ml. Serum CYFRA21-1 concentration elevated in 5 of 10 esophageal cancer patients, 5 of 10 gastric cancer patients, 7 of 10 colorectal cancer patients and 6 of 10 pancreatic cancer patients. Positive rate in patients with benign disease including hepatopathy was low. BALL ELSA CYFRA21-1 kit is reliable and CYFRA21-1 could be a useful tumor marker in gastrointestinal cancer.

Asunto(s)

Antígenos de Neoplasias/sangre , Biomarcadores de Tumor/sangre , Ensayo Inmunorradiométrico/métodos , Queratinas/sangre , Juego de Reactivos para Diagnóstico/normas , Adulto , Anticuerpos Monoclonales , Estudios de Evaluación como Asunto , Femenino , Humanos , Queratina-19 , Masculino , Persona de Mediana Edad , Neoplasias/diagnóstico

RESUMEN

The concentration of carcinoembryonic antigen (CEA), CA130, CA125, SLX, CA19-9, SPan1, and tumour-associated glycoprotein 72 (TAG-72) in the culture supernatant of 15 cancer cell lines and in the sera of 58 cancer patients was measured, and the co-expression of these antigens was examined by double determinant immunoradiometric assays. The high correlation coefficient of the concentrations and significant binding in the double determinant assays indicated a close relationship between CA125 and CA130 and between CA19-9 and SPan1. There was variable binding of the 125I-labelled anti-SLX, anti-CA19-9, and anti-SPan1 antibodies to anti-CA130 beads that had been pre-incubated with the culture supernatants, suggesting the presence of the epitopes of SLX, CA19-9, and SPan1 on the molecule expressing CA130. Similarly, the epitopes of SLX, CA19-9, and SPan1 could be present on the molecule expressing CEA. 125I-labelled anti-CA19-9, anti-SLX, and anti-TAG-72 antibodies were bound in variable proportions to anti-CA130 beads or to anti-CEA beads that had been pre-incubated with patients' sera. However, CEA and CA130 were not expressed on the same molecule, either in the culture supernatant, or in patients' sera. In conclusion, the carbohydrate epitopes of CA19-9, SPan1, SLX, and TAG-72 could be present on the molecule recognised by the anti-CA130 or anti-CEA antibody; however, the epitopes of CA130 and CEA did not co-exist on the same molecule.

Asunto(s)

Anticuerpos Monoclonales/inmunología , Antígenos de Neoplasias/análisis , Biomarcadores de Tumor/análisis , Antígenos de Carbohidratos Asociados a Tumores/análisis , Antígeno Carcinoembrionario/análisis , Glicoproteínas/análisis , Humanos , Células Tumorales Cultivadas