RESUMEN
This experiment was conducted to establish a system for detecting trace cobalt ions in water and plant tissues using a voltammetric in vivo sensor. Cyclic and stripping voltammetry was devised from hand-made, macro-type implantable three-electrode systems. The results reached micro and nano working ranges at 100 sec accumulation time. The statistical detection limit (S/N) was attained at 6.0 ng L(-1). For the in vivo application, direct assay of cobalt ions was carried out in Eichhornia crassipes (EC) deep tissue in real time with a preconcentration time of 100 s. Interfaced techniques can be interlocked with other control systems.
Asunto(s)
Cobalto/análisis , Eichhornia/metabolismo , Electrodiagnóstico , Cobalto/metabolismo , ElectrodosRESUMEN
The 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect and total phenolic contents were evaluated for the screening of singlet oxygen ((1)O(2)) quenching efficacy of various seed extracts from Rumex crispus L. The butanol and ethyl-acetate extracts displayed remarkable effect of DPPH as compared to positive control ascorbic acid. The concentrations (QC(50)) of butanol and ethyl-acetate extracts required to exert 50% reducing effect on (1)O(2) were found to be 116 and 82 µg mL(-1), respectively. Both extracts were also found to protect the in vitro biological system from the detrimental effect of (1)O(2) on type II photosensitization in Escherichia coli, red blood cell, lactate dehydrogenase and histidine. Among all the tested extracts, the ethyl-acetate and butanol extracts contained higher amount of total phenolic contents. The results suggest that our study may contribute to the development of new bioactive products with potential applications to reduce photo-produced oxidative stress involving reactive oxygen species in living organisms.