RESUMEN
The use of the dietary supplement SeleniumEC resulted in the normalization of markers of the lipid peroxidation and the antioxidant system during complex diet therapy of patients with ischemic heart diseases. These markers were more stable in patients received SeleniumEC further one month after the period in the clinic.
Asunto(s)
Antioxidantes/metabolismo , Enfermedades Cardiovasculares/terapia , Suplementos Dietéticos , Selenio/uso terapéutico , Anciano , Enfermedades Cardiovasculares/sangre , Humanos , Masculino , Persona de Mediana EdadRESUMEN
The article discusses the biochemical mechanisms of skin destruction and ulcer formation in patients with varicosity of the lower limbs. Cathepsins A, B, C, and D were determined in the skin in various parts of the limb in 57 patients: in the lower third of the leg the activity of cathepsin D was increased by 183.8%, that of cathepsin B by 140.2%, and the activity of cathepsin B by 239%. On basis of the data obtained the authors conclude that cathepsins take part in skin destruction. Increased activity of cathepsin D plays the initiative role in this process. Cathepsin activity reduced after 14-16 day treatment with aescusan; D by 24.1%, B by 17.7%, and A by 15.4%. The authors link the effect of the treatment with the protective effect of the preparation on the lysosomal membranes.
Asunto(s)
Catepsinas/metabolismo , Piel/enzimología , Úlcera Varicosa/etiología , Várices/complicaciones , Activación Enzimática/fisiología , Humanos , Várices/enzimologíaRESUMEN
Platelet lysosomal proteinase and phospholipase activity, cholesterol content in the blood serum and circulating immune complexes, as well as blood serum atherogenic potential were studied with the use of human aortal intima atherosclerotic cell culture in patients with postinfarction cardiosclerosis (males aged from 45 to 62 years), 1-3 h before and 2-6 h after a single intake of one of the following products (50 g): sunflower oil, butter, cod fat, glucose, fructose, starch, dried milk protein, or 22 g of soybean protein isolate 500 U. The total data obtained have evidenced that the soybean protein isolate produces hypolipidemic and hypocholesterolemic effects, as well as possesses pronounced anti-atherosclerotic properties.
Asunto(s)
Arteriosclerosis/dietoterapia , Alimentos , Plaquetas/enzimología , Catepsina B/metabolismo , Catepsina C , Colesterol en la Dieta/administración & dosificación , Carbohidratos de la Dieta/administración & dosificación , Proteínas en la Dieta/administración & dosificación , Dipeptidil-Peptidasas y Tripeptidil-Peptidasas/metabolismo , Humanos , Masculino , Persona de Mediana Edad , Músculo Liso Vascular/análisis , Fosfolipasas A/metabolismo , Proteínas de Vegetales Comestibles/administración & dosificación , Proteínas de SojaRESUMEN
Investigation of lysosomal hydrolase activity in platelets of patients has revealed drastic activation of cathepsins B, C and phospholipase A1, the degree of which rose in the following range: coronary heart disease; coronary heart disease aggravated by obesity: obesity and hyperlipidemia (type II). Administration of the adequate dietotherapy resulted in normalization of enzymologic parameters, whereas the results of the clinico-biochemical analysis of the blood were less informative in all cases. The data obtained could be used in the evaluation of the dietotherapy effectiveness, as well as for the early diagnosis of the corresponding diseases.
Asunto(s)
Plaquetas/enzimología , Enfermedad Coronaria/enzimología , Hidrolasas/sangre , Hiperlipidemias/enzimología , Lisosomas/enzimología , Fenómenos Fisiológicos de la Nutrición , Obesidad/enzimología , Adulto , Catepsinas/sangre , Enfermedad Coronaria/dietoterapia , Dieta Reductora , Humanos , Hiperlipidemias/dietoterapia , Persona de Mediana Edad , Obesidad/dietoterapia , Fosfolipasas/sangreAsunto(s)
Plaquetas/enzimología , Enfermedad Coronaria/dietoterapia , Hidrolasas/sangre , Hiperlipidemias/dietoterapia , Lisosomas/enzimología , Adulto , Enfermedad Coronaria/enzimología , Grasas de la Dieta/administración & dosificación , Proteínas en la Dieta/administración & dosificación , Ingestión de Energía , Femenino , Humanos , Hiperlipidemias/enzimología , Masculino , Persona de Mediana EdadRESUMEN
The activity of cathepsins A, B, C, D, phospholipases A1 and A2, and aryl sulphatases A and B was studied in hepatic lysosomas, adipocytes of epididymal fatty tissue and in platelets of rats aged 2,5 and 24 months differing in the character of milk feeding. It was found that excessive feeding in the neonatal period resulted in a decrease of the lysosomal proteinase activity by 18-33% in 24-month animals, while phospholipase A2 activity rose 1,4-2.2-fold. Phospholipase A2 activity proved to be also increased in adipocytes of obese rats. Obese rats' platelets were characterized by a drastic (2-3.5-fold) activation of cathepsin C, and phospholipase A1 activity rose by 55% at all the stages of the ontogenesis. It is suggested that the changes in the lysosomal hydrolases activity may reflect the platelet function in the disordered lipoprotein metabolism.