RESUMEN
In experiments in vitro and in vivo influence of formiate on free amino acids contents of tissues and organs of rats has been studied. Results of experiments showed a possibility of amino acids formilation in all investigated tissues. This process could be enzymatic. Its effectivness depended on amino acids and formiate concentrations. The most effective processes took place in organs with high metabolic activity.
Asunto(s)
Aminoácidos/metabolismo , Formiatos/farmacología , Animales , Relación Dosis-Respuesta a Droga , Técnicas In Vitro , Masculino , Especificidad de Órganos , RatasRESUMEN
Acetic and succinate acids KoA acyl derivatives interacting with formate were displayed to produce alpha-ketoacids--pyruvate and alpha-ketoglutarate. These acids also interact with formate and make pyruvic and malate acids, while alpha-ketoglutarate, evidently, tricarboxy acids. Interaction of formate with acetic and succinate acids inspite of occurring out of the tricarbone cycle increases the latter metabolic functions.
Asunto(s)
Ácido Acético/metabolismo , Formiatos/metabolismo , Ácido Succínico/metabolismo , Animales , Ácidos Cetoglutáricos/metabolismo , Ácido Pirúvico/metabolismoRESUMEN
Emotional stress of rats is accompanied by essential alterations in glycolysis and tricarboxylic acid cycle in the liver. These alterations were observed over a few days after the termination of stressor action. Perhaps it is one of the trigger mechanisms responsible for pathological changes in the organism.
Asunto(s)
Hígado/metabolismo , Estrés Psicológico/metabolismo , Animales , Ciclo del Ácido Cítrico , Glucólisis , Masculino , RatasAsunto(s)
Adaptación Fisiológica/efectos de la radiación , Adaptación Psicológica/efectos de la radiación , Encéfalo/efectos de la radiación , Adulto , Encéfalo/fisiopatología , Electroencefalografía , Femenino , Actividad Nerviosa Superior/fisiología , Actividad Nerviosa Superior/efectos de la radiación , Humanos , Estudios Longitudinales , Masculino , Persona de Mediana Edad , Exposición Profesional/efectos adversos , Centrales Eléctricas , Psicopatología , Liberación de Radiactividad Peligrosa/psicología , UcraniaRESUMEN
The intensity of biosynthesis processes in animal organism has been studied as affected by long-term administration of morphine. It was established that morphine administration to rats for five weeks intensified protein biosynthesis in the brain, kidneys, skeletal muscles: specific radioactivity of blood serum proteins also increased. Incorporation of 2-/14C/glycine label to the brain, cardiac and skeletal muscles increased as affected by morphine: the label incorporation to the liver lipids decreased and that to the kidney and spleen lipids did not change. Specific radioactivity of glycogen multiply increased in the rat liver as affected by morphine.
Asunto(s)
Glucógeno/biosíntesis , Lípidos/sangre , Morfina/administración & dosificación , Biosíntesis de Proteínas , Animales , Proteínas Sanguíneas/efectos de los fármacos , Proteínas Sanguíneas/metabolismo , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Corazón/efectos de los fármacos , Riñón/efectos de los fármacos , Riñón/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , Músculos/efectos de los fármacos , Músculos/metabolismo , Miocardio/metabolismo , Ratas , Ratas Wistar , Bazo/efectos de los fármacos , Bazo/metabolismo , Factores de TiempoRESUMEN
Administration of morphine into rats at a dose of 30 mg/kg within 6 days led to a decrease in total rate of tRNA aminoacylation in liver tissue. Content of lactate, pyruvate, malate and alpha-ketoglutarate was decreased within 6 days-long course of morphine administration, while content of lactate was only altered after 5 weeks of the intoxication. Adaptation reactions appear to be increased with time in long-term intoxication with morphine.
Asunto(s)
Hígado/metabolismo , Morfina/envenenamiento , Animales , Ciclo del Ácido Cítrico , Metabolismo Energético , Glucólisis , Masculino , Biosíntesis de Proteínas , Aminoacil-ARN de Transferencia/metabolismo , Ratas , Ratas WistarRESUMEN
Formate was studied for its effect on the content of acetaldehyde, activity of the total aldehyde dehydrogenase, content of substrates of glycolysis and tricarbonic-cycle and pool of free amino acids of rat tissues during alcohol intoxication. The introduction of formate during the acute alcohol intoxication lowers the acetaldehyde content in the blood; the ethanol load being prolonged--it increases the activity of aldehyde dehydrogenase and normalizes the content of pyruvate, glutamate and malate in the liver and glutamate and oxaloacetate in the brain, that evidences for the correction of metabolic disturbances in the organism.
Asunto(s)
Intoxicación Alcohólica/metabolismo , Alcoholismo/metabolismo , Formiatos/farmacología , Acetaldehído/sangre , Aldehído Deshidrogenasa/antagonistas & inhibidores , Aldehído Deshidrogenasa/metabolismo , Aminoácidos/sangre , Animales , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Formiatos/sangre , Formiatos/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Masculino , RatasAsunto(s)
Alanina Transaminasa/sangre , Amoníaco/sangre , Aspartato Aminotransferasas/sangre , Carbonatos/farmacología , Diabetes Mellitus Experimental/sangre , Glutamina/sangre , Sulfato de Magnesio/farmacología , Compuestos de Manganeso , Manganeso/farmacología , Compuestos de Zinc , Zinc/farmacología , Animales , Análisis de los Gases de la Sangre , Combinación de Medicamentos/farmacología , ConejosRESUMEN
Application of carbostimulin, the preparation stimulating CO2 fixation in tissues, in a complex therapy of diabetes mellitus patients rises the CO2 level, restores the total content of alpha-ketoacids and free amino acids in blood, increases (within the physiological normal limits) the urea content in blood and urine of diabetes mellitus patients.
Asunto(s)
Carbonatos/uso terapéutico , Diabetes Mellitus/tratamiento farmacológico , Sulfato de Magnesio/uso terapéutico , Compuestos de Manganeso , Manganeso/uso terapéutico , Compuestos de Zinc , Zinc/uso terapéutico , Aminoácidos/sangre , Dióxido de Carbono/sangre , Diabetes Mellitus/metabolismo , Combinación de Medicamentos/uso terapéutico , Humanos , Cetoácidos/sangre , Urea/metabolismoRESUMEN
In rabbits with alloxane diabetes the intensity of [I-14C] glycine label incorporation into various tissue components is considerably lowered. Stimulation of carboxylation in animals under these conditions intensifies the label incorporation into proteins of the liver, kidneys, spleen as well as into blood serum albumin and globulin. The level of lipids, glycogen in the liver, the total content of proteins in blood serum and their specific radioactivity increase. All this evidences for intensification of biosynthetic processes and for a possibility to normalize the metabolism with diabetes by stimulating the processes of carboxylation.
Asunto(s)
Carbonatos/farmacología , Diabetes Mellitus Experimental/metabolismo , Glicina/metabolismo , Lípidos/biosíntesis , Sulfato de Magnesio/farmacología , Compuestos de Manganeso , Manganeso/farmacología , Biosíntesis de Proteínas , Compuestos de Zinc , Zinc/farmacología , Animales , Proteínas Sanguíneas/biosíntesis , Combinación de Medicamentos/farmacología , Riñón/metabolismo , Hígado/metabolismo , Glucógeno Hepático/biosíntesis , Conejos , Bazo/metabolismoRESUMEN
Feeding carboxylin and sodium citrate to rabbits with alloxane diabetes, normalizes the disturbed contents of malate, alpha-ketoglutarate, oxaloacetate, citrate and pyruvate in the blood and liver of these animals restores the total content of alpha-keto-and free amino acids, increases considerably the urea content in the liver.