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Context: Shigella is a common cause of bacillary dysentery. Although it is reported worldwide, the majority of the infections are seen in developing countries with Shigella flexneri being the most common isolate. Prevalence of Shigella species and their antibiotic susceptibility profiles vary according to geographic area and season. Aims: In the present study, the epidemiology and antimicrobial profile of Shigella from stool samples received at our hospital for a period of 12 years (January 2006 to December 2017) was evaluated. Subjects and Methods: A total of 4578 stool samples were collected from the cases of acute gastroenteritis and diarrhoea. Samples were processed for culture and sensitivity according to standard microbiological techniques. The presumptive identification of Shigella species was done using standard conventional biochemical tests and confirmed using antisera. Results: A total of 189 (4.2%) samples yielded Shigella spp. Isolation of Shigella spp. were more frequent from males (58.2%). S. flexneri was the commonest species isolated (47.6%) followed by Shigella sonnei(11.6%), Shigella dysenteriae (4.2%) and Shigella boydii (2.1%). Non-typeable Shigella was commonly recovered. The isolates showed high resistance to ampicillin (76.7%) and co-trimoxazole (75%) while highest susceptibility was observed to ceftriaxone (79.2%). Conclusions: S. flexneri was the most prevalent species isolated at this centre. Shigella isolates from the study showed alarming resistance to recommended antibiotics. Non-typeable Shigella accounted for 34.4% isolates. Molecular discrimination between Shigella and Escherichia coli is essential.
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Farmacorresistencia Bacteriana Múltiple , Disentería Bacilar/epidemiología , Disentería Bacilar/microbiología , Shigella/clasificación , Shigella/aislamiento & purificación , Adolescente , Adulto , Anciano , Niño , Preescolar , Heces/microbiología , Femenino , Humanos , India/epidemiología , Lactante , Masculino , Persona de Mediana Edad , Prevalencia , Centros de Atención Terciaria , Adulto JovenRESUMEN
INTRODUCTION: Extra-Pulmonary Tuberculosis (EPTB) accounts for approximately 40% of the tuberculosis cases. Though it is not communicable, it is a significant cause of morbidity. This study was conducted to know the efficacy of the Polymerase Chain Reaction (PCR) as an additional tool, along with the conventional methods, in the diagnosis of EPTB. MATERIAL AND METHODS: Clinical samples were collected from suspected cases of EPTB. The Ziehl-Neelsen staining (ZNS), culture on the Lowenstein-Jensen medium (LJM) and PCR testing with the use of a commercial kit were performed on the homogenized samples. RESULTS: A total of 182 samples which were received for the molecular diagnosis of EPTB were also tested by ZNS and culture on LJM for the presence of Mycobacterium tuberculosis. Of these, 22 were positive by at least one of the tests which were used. PCR detected the maximum number of cases of EPTB, followed by culture. The results of PCR and the conventional tests were analyzed by using McNemar's test for the correlated proportions-the exact method of 'IBM SPSS Statistics 20'. The analysis showed a statistical significance. CONCLUSIONS: Whenever they are feasible, using all the available tests in combination increases the laboratory detection rates of M. tuberculosis from clinical samples. PCR must be included in the diagnostic panel of EPTB.
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BACKGROUND: Chromoblastomycosis is a chronic fungal infection which presents as a small ulcer, plaques, papulonodular lesion to cauliflower-like warty masses and cicatricial forms. The disease is relatively uncommon, with only a few published case report. METHODS: A 35-year-old female presented with phagedenic ulcer on the face since 15 years. Examination identified a plaque with central perforation on the right side of the face with crusting and oozing and a scar at the periphery with visible underlying turbinates. A biopsy was done and sent for histopathologic examination and culture. Treatment was started with oral fluconazole, 150 mg daily orally, suspecting subcutaneous fungal infection. RESULTS: Biopsy revealed branched, septate, brown-colored dematiaceous hypae in the dermis indicating chromoblastomycosis. Fungal culture revealed Fonsecaea pedrosoi. Patient responded well to treatment, and still she is on treatment. CONCLUSIONS: We believe that this is the first case report of chromoblastomycosis presenting as phagedenic ulcer.