RESUMEN
The number, proliferative potential and differentiation potentialities of bone marrow granulocytic-macrophagal precursors were investigated in 130 ALL patients (77 children in the acute period of disease, 53 children in remission) and in the bone marrow of 65 controls without hematologic pathology. A decrease in the number of clonogenic precursors was observed in all stages of acute lymphoblastic leukemia, the decrease being particularly marked in the acute period of disease characterized by substantial bone marrow infiltration by leukemic cells. A decrease in the number of precursors and their proliferative potential was shown to be associated with the influence of leukemic blasts. In remission the numerical reduction of the precursor pool was determined by a cytostatic therapeutic effect. The ability to differentiation of granulocytic and macrophagal clonogenic precursors in ALL children was unchanged.
Asunto(s)
Granulocitos/citología , Hematopoyesis , Leucemia-Linfoma Linfoblástico de Células Precursoras/patología , Adolescente , Protocolos de Quimioterapia Combinada Antineoplásica/uso terapéutico , Células de la Médula Ósea , Niño , Preescolar , Ensayo de Unidades Formadoras de Colonias , Femenino , Granulocitos/efectos de los fármacos , Hematopoyesis/efectos de los fármacos , Humanos , Lactante , Recuento de Leucocitos , Macrófagos/citología , Macrófagos/efectos de los fármacos , Masculino , Leucemia-Linfoma Linfoblástico de Células Precursoras/tratamiento farmacológico , Inducción de Remisión , Células Tumorales CultivadasAsunto(s)
Células de la Médula Ósea , Granulocitos/citología , Células Madre Hematopoyéticas/citología , Macrófagos/citología , Adolescente , Recuento de Células , Niño , Preescolar , Ensayo de Unidades Formadoras de Colonias , Enfermedad de Hodgkin/patología , Humanos , Lactante , Púrpura Trombocitopénica/patologíaAsunto(s)
Granulocitos/inmunología , Leucemia Linfoide/inmunología , Macrófagos/inmunología , Adolescente , Niño , Preescolar , Femenino , Humanos , Masculino , Inducción de RemisiónRESUMEN
The colony stimulating activity (CSA) of bone marrow cells in 50 children with normal bone marrow was studied. A method for quantitative estimation of CSA of bone marrow cells has been suggested. Essential distinctions between CSA of bone marrow cells and that of peripheral blood leukocytes were established considerable decrease mainly at the expense of reduction of the proliferation of colony-forming progenitors was found. CSA of bone marrow cells appears to be a result of complicated interaction between bone marrow adherent and non-adherent fractions. Each of these two fractions produces approximately the similar level of CSA which is lower than that of unfractionated bone marrow. It is supposed that there are two types of granulocyte macrophage progenitors in the human bone marrow which are specifically-sensitive to CSA of leukocytes and bone marrow cells and possess different proliferative potential.