RESUMEN
The activity of NADPH-cytochrome c-reductase, benzpyrene hydroxylase, epoxy-hydratase and glutathione-S-transferase in human peripheral blood lymphocytes was studied. In the presence of NADPH, native lymphocytes were unable to reduce cytochrome c. In order to improve the availability of substrates for enzymes, lymphocytes were degraded by single-stage freezing-melting. At the same time, the activities of NADPH-cytochrome c-reductase, epoxide hydratase, and glutathione-S-transferase were 1.7 +/- 0.6, 49.0 +/- 18.0, and 30.0 +/- 6.0 nmol/min per mg protein, respectively. The lymphocytic levels of cytochrome P-450 were approximately 0.1-0.2 nmol per mg microsomal protein, while those of cytochrome b5 were nearly 0.5 nmol/mg microsomal protein in the lymphocytes.
Asunto(s)
Linfocitos/enzimología , Xenobióticos/metabolismo , Benzopireno Hidroxilasa/metabolismo , Sistema Enzimático del Citocromo P-450/metabolismo , Citocromos b5/metabolismo , Epóxido Hidrolasas/metabolismo , Glutatión Transferasa/metabolismo , Humanos , NADH Deshidrogenasa/metabolismoAsunto(s)
Bolsa de Fabricio/fisiología , Frío/efectos adversos , Péptidos/farmacología , Animales , Pollos , Femenino , Inmunidad Innata/efectos de los fármacos , Inyecciones Intraperitoneales , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Péptidos/aislamiento & purificación , SolucionesRESUMEN
The specific polysaccharide was obtained from the lipopolysaccharide of Shigella newcastle by mild acid hydrolysis and further purified by permeation chromatography on Sephadex G-50. It was found to consist of L-rhamnose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid residues and O-acetyl groups in the molar ratios of 2:1:1:1. On the basis of 1H and 13C nuclear magnetic resonance spectroscopy, methylation analysis, partial acid hydrolysis, Smith degradation, and chromium trioxide oxidation, the following structure can be assigned to the repeating oligosaccharide unit of the polysaccharide:-4)DGalA(beta 1-3)DGalNAc-(beta 1-2)LAc3Rha(alpha 1-2)LRha(alpha 1-, where GalA = galacturonic acid. GalNAc = N-acetylgalactosamine, Ac3Rha = 3-O-acetylrhamnose. The structural and immunochemical data presented prove that Sh. newcastle lipopolysaccharide belongs to a 'non-classical' type of somatic antigens with acidic O-specific polysaccharide chains.