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The paper probed into corporate governance and the feasibility of introducing corporate governance into China' s public hospitals based on the principal-agent theory and successful experiences of overseas hospitals.It also described the incentive and restriction mechanism of such governance.The authors proposed such key factors as evolution in property right/shareholding relationship,organization structure of introducing corporate governance,appropriate supervision,sufficient competition,and perfecting corporate governance mechanism.
RESUMEN
Objective To construct a 5-lipoxygenase (5-LO) transgenic mouse model of atherosclerosis.Methods Purified 5-LO fragment was injected into male pronucli and the firtilized eggs were transplanted into pseudopregnant mice.PCR and Southern blot were used to detect the genotype of DNA separated from the newborn mouse tail tissues.RT-PCR and Western blot analysis were used to detect the gene transcription and expression.Results PCR and Southern blot results showed that 7 of 25 mice were transgenic mice.Expression of 5-LO and FLAP was found in the bone marrow,spleen,kidney,and peritoneal cells.Results of RT-PCR and Western blot showed that No.9,20,24transgenic mice expressed a higher level of 5-LO and FLAP than those in the wild type C57BL/6 mice.The expression levels in bone marrow and peritoneal cells were significantly different(P<0.05).Conclusion A 5-LO transgenie mouse line has been established in this study and may be used for future study on the function of 5-LO gene.