RESUMEN
Under conditions of COVID-19 pandemic, considerable amounts of SARS-CoV-2 contained in household, municipal, and medical wastewaters inevitably reach natural water bodies. Possible preservation of virus infectivity in liquid environment is of a paramount epidemiological importance. Experiments demonstrated that SARS-CoV-2 is resistant to multiple freezing/thawing cycles and retains its infectivity in tap and river water for up to 2 days at 20°C and 7 days at 4°C. In natural milk, its viability is preserved in a refrigerator for 6 days. The exposure of aquarium fish to the virus-containing water fails to cause any infection.
Asunto(s)
COVID-19 , Animales , Humanos , Pandemias , SARS-CoV-2 , Aguas Residuales , AguaRESUMEN
Different methods for producing bulk quantities of concentrated and purified SARS-CoV-2 for the use as antigens and for the research into COVID-19 biology were tested.
Asunto(s)
Prueba de COVID-19/métodos , COVID-19/diagnóstico , SARS-CoV-2/inmunología , Carga Viral/inmunología , Virión/inmunología , Animales , Anticuerpos Neutralizantes/aislamiento & purificación , Anticuerpos Neutralizantes/metabolismo , Anticuerpos Antivirales/aislamiento & purificación , Anticuerpos Antivirales/metabolismo , COVID-19/virología , Línea Celular , Chlorocebus aethiops , Convalecencia , Células Epiteliales/virología , Humanos , Sueros Inmunes/química , SARS-CoV-2/genética , SARS-CoV-2/crecimiento & desarrollo , Porcinos , Células Vero , Carga Viral/genética , Proteínas Virales/inmunología , Proteínas Virales/aislamiento & purificación , Proteínas Virales/metabolismo , Virión/genética , Virión/crecimiento & desarrollo , Replicación ViralRESUMEN
The effects of recombinant analog of natural Ebola virus protein vp24 in configurations virulent (vp24-ad) and avirulent (vp24-w) for guinea pigs on interferonogenesis were studied in vivo and in vitro. Amino acid differences were determined by His186 substitution in avirulent (nonlethal) configuration for Tyr in the virulent (lethal) one. Recombinant analogs vp24-w and vp24-ad inhibited interferonogenesis in vivo and in vitro. Inhibition by the two protein configurations was virtually the same.