RESUMEN
Concentrations of Hg, Se, Cd, Cu, Zn, Fe, Mn and As, in kidney, liver, muscle and blubber from 7 specimens of Stenella coeruleoalba, stranded along the Israeli Mediterranean coast (IMC) from 2006 to 2011 (2011-series) were determined and compared to previous data on S. coeruleoalba from the IMC (2001-series). No differences were observed in essential and toxic elements concentrations, between the two series, except for hepatic Mn which was higher in the latter. Hg/Se molar ratios in blubber, kidney and liver increased linearly with log Hg concentrations, while muscle was more heterogenic in this respect. Means (±SD) of hepatic Hg concentrations (134±89 and 181±200mgkg(-1), from the 2011 and 2001 series, respectively) were similar to that found in 2007-2009 specimens from Spain, possibly reflecting the relatively high natural background levels of mercury in the Mediterranean Sea.
Asunto(s)
Monitoreo del Ambiente/métodos , Metales Pesados/análisis , Stenella/metabolismo , Oligoelementos/análisis , Contaminantes Químicos del Agua/análisis , Tejido Adiposo/química , Animales , Femenino , Riñón/química , Hígado/química , Masculino , Mar Mediterráneo , Mercurio/análisis , Mercurio/farmacocinética , Metales Pesados/farmacocinética , Selenio/análisis , Selenio/farmacocinética , España , Oligoelementos/farmacocinética , Contaminantes Químicos del Agua/farmacocinéticaRESUMEN
This study provides, for the first time, a baseline evaluation of dioxin-like biological activity in sediments and fish sampled in- and adjacent to anchorages along the Mediterranean and Red Sea coasts of Israel. It indicates the effect of past pollution, still present in the sediments of older Israeli harbors, with putative contribution of still existing sources of pollution. A commercial reporter gene bioassay was used to evaluate the biological activity of dioxin-like compounds extracted from the samples. HRGC/HRMS analysis of several samples contributed a profile of dioxin-like compounds in sediments and fish. The results point out 1,2,3,4,6,7,8-HeptaCDD, 2,3,4,6,7,8-HexaCDF, 1,2,3,4,6,7,8-HeptaCDF, РСÐ-126 and РСÐ-118 as major contributors to the dioxin-like activity in sediments. It indicates polychlorinated biphenyls non-selective absorption in fish livers, in contrary to a biased accumulation of poorly chlorinated and more potent dibenzodioxins and dibenzofurans.
Asunto(s)
Bioensayo/métodos , Dioxinas/toxicidad , Peces , Sedimentos Geológicos/análisis , Hígado/química , Contaminantes Químicos del Agua/análisis , Animales , Benzofuranos/farmacocinética , Benzofuranos/toxicidad , Dioxinas/farmacocinética , Monitoreo del Ambiente , Genes Reporteros , Sedimentos Geológicos/química , Océano Índico , Israel , Mar Mediterráneo , Bifenilos Policlorados/análisis , Bifenilos Policlorados/farmacocinética , Bifenilos Policlorados/toxicidad , Extractos de Tejidos/análisis , Extractos de Tejidos/química , Extractos de Tejidos/toxicidad , Contaminantes Químicos del Agua/farmacocinética , Contaminantes Químicos del Agua/toxicidadRESUMEN
Efficient implementation of an environmental biomarker requires multi-annual comparability over a wide geographical range. The present study improved the comparability of a quantitative competitive metallothionein (MT) enzyme-linked-immuno-sorbent-assay (ELISA) in the sentinel fish Lithognathus mormyrus by introducing to the assay recombinant MT and beta-actin standards. Commercial antibodies for cod MT and mammalian actin were implemented. In addition, a sensitive anti L. mormyrus MT antibody was produced, adequate only for solid phase immunochemical assays. Cadmium was applied to the fish through injection and feeding to serve as a testing platform of the ELISA. The results demonstrated high potential protective capacity of the liver against toxic levels of transition metals through increasing MT levels. MT transcript levels were evaluated also from fish sampled at polluted and relatively clean natural sites, indicating applicability of MT as biomarker of exposure to a multi-factorial pollution, in comparison to its low revealed sensitivity to controlled cadmium exposure.
Asunto(s)
Cadmio/toxicidad , Metalotioneína/análisis , Metalotioneína/inmunología , Perciformes/metabolismo , Contaminantes del Agua/toxicidad , Actinas/genética , Actinas/metabolismo , Animales , Biomarcadores , Cadmio/metabolismo , Ecosistema , Monitoreo del Ambiente , Ensayo de Inmunoadsorción Enzimática/métodos , Hígado/química , Metalotioneína/biosíntesis , Metalotioneína/genética , Perciformes/genética , Proteínas Recombinantes/inmunología , Contaminantes del Agua/metabolismoRESUMEN
Hepatic cytochrome P4501A (CYP1A) expression was partially characterized in the striped sea bream (Lithognathus mormyrus) from the Mediterranean coast of Israel as part of the process of establishing the CYP1A gene as an environmental biomarker. Reverse transcription-competitive polymerase chain reaction, competitive enzyme-linked immunosorbent assay, and ethoxyresorufin O-deethylase (EROD) assay were used for evaluating transcript, protein, and catalytic activity levels, respectively, in absolute units. Highest elucidated transcript, protein, and catalytic activity levels were 0.264 +/- SD 0.084 fmol/microg total RNA, 0.88 +/- 0.52 pmol/microg total protein, and 1.11 +/- 0.52 pmol resorufin/min/microg total protein, respectively, and the lower levels were 0.009 +/- 0.007 fmol/microg total RNA, 0.17 +/- 0.08 pmol/microg total protein, and 0.11 +/- 0.06 pmol resorufin/min/microg total protein, respectively, demonstrating substantial induction potential. All alternate pairs of seven examined field samples, revealing a transcript-level ratio higher than 1.7, also demonstrated a significant difference between their transcript levels, indicating a potential to detect relatively small biomarker changes (1.7-fold) caused by environmental effects. Simultaneous triple measurements of transcript, protein, and catalytic activity were carried out in individuals from two field samples and during a 318-d decay experiment. Fish from the field samples revealed significant alternate bivariate correlation between transcript, protein, and enzymatic activity. Conflicting results were found when analyzing the decay experiment, in which both protein and catalytic activity levels decreased significantly to basal levels, in contrast to no significant change in transcript levels throughout the experiment. No significant difference was observed between males and females regarding the levels of CYP1A transcript, protein, and EROD.