RESUMEN
A clinical trial has been performed of the food Unipit-3 and Laktanal based on cow milk proteins subjected to enzymic hydrolysis, dia- and ultrafiltration. A preliminary immunochemical assay of the above products protein component and its testing in anaphylactic shock reaction in animals demonstrated low sensitizing activity of this protein. The results of the trial of the above hypoallergenic foods in patients with food allergy showed their good tolerance and therapeutic efficacy.
Asunto(s)
Enfermedades del Sistema Digestivo/dietoterapia , Hipersensibilidad a los Alimentos/dietoterapia , Complejo Antígeno-Anticuerpo/análisis , Carbohidratos de la Dieta/administración & dosificación , Grasas de la Dieta/administración & dosificación , Proteínas en la Dieta/administración & dosificación , Enfermedades del Sistema Digestivo/inmunología , Hipersensibilidad a los Alimentos/inmunología , HumanosRESUMEN
The capacities of immunoelectrophoresis in identification and assay of antigenic protein in enzymatic hydrolysates of milk and soya have been evaluated. The hydrolysates obtained under the action of pancreatin and protosubtilin were studied in milk protein concentrates "Dialact" and casezit and soya protein isolate "Adrex". Two-dimensional immunoelectrophoresis according to Clarke and Freeman was used to analyze the antigenic component spectrum in the samples. It has been shown that beta-lactoglobulin, as compared to other milk proteins, is most resistant to pancreatin-induced proteolysis in vitro. Pancreatin-induced proteolysis has been shown to be useful for trypsin inhibitor inactivation in soya protein isolate. It was demonstrated that immunoelectrophoresis could be used for complex evaluation of protein hydrolysates intended for manufacturing specialized foodstuffs.