RESUMEN
59 angiomyolipomas were studied: immunohistochemistry (IHC) was used in 24 cases, electron microscopy (EM) in 6 cases. 57 tumors had a typical structure, 2 did not contain fat component. Premelanosomes were found by EM in one tumor. Smooth muscle actin was found in all tumor cells. Involvement of lymph nodes was observed in 2 patients. Histogenesis of this tumor is discussed.
Asunto(s)
Angiomiolipoma/ultraestructura , Neoplasias Renales/ultraestructura , Riñón/ultraestructura , Melanosomas/ultraestructura , Actinas , Adolescente , Adulto , Anciano , Angiomiolipoma/metabolismo , Femenino , Humanos , Riñón/metabolismo , Neoplasias Renales/metabolismo , Ganglios Linfáticos/metabolismo , Ganglios Linfáticos/ultraestructura , Masculino , Melanosomas/metabolismo , Persona de Mediana EdadRESUMEN
The content of total T- and B-lymphocytes, T-rosette-forming at 37 degrees, T-autorosette-forming (ARF), FcIgM+, FcIgG+ and TDT+ lymphocytes is determined in mice after unilateral nephrectomy. The number of ARF, FcIgM+ and TDT+ lymphocytes increased 17 and 48 hours after the nephrectomy. The coincidence in time of the lymphocyte population increase with acquisition of morphogenetic activity by the spleen lymphoid cells allows one to assume their participation in the regenerative proliferation of renal epithelium. The alterations in the content of other lymphocyte populations were in the limit of random deviations.
Asunto(s)
Riñón/fisiología , Subgrupos Linfocitarios/metabolismo , Bazo/patología , Animales , Cinética , Ratones , Morfogénesis , Nefrectomía , Periodo Posoperatorio , Regeneración/fisiología , Formación de RosetaRESUMEN
Changes in primordial lymphoid organs (bone marrow and thymus) with respect to the content of a serum thymic factor in the blood and specific activity of end desoxynucleotidyltransferase in the bone marrow and peripheral blood were studied in patients with chronic glomerulonephritis with and without the nephrotic syndrome. The mechanisms of autoimmunization were also studied with respect to the presence of renal tissue autoantigen antibodies. Alteration of primordial lymphoid organs was not attended by the autoimmune changes, i.e. production of the renal autoantigen antibodies was not increased. This makes difficult interpretation of the nephrotic syndrome as an autoimmune process and of glomerulonephritis as a whole. Lymphocytes bearing end desoxynucleotidyltransferase more probably participate in the developmental mechanisms of chronic glomerulonephritis.
Asunto(s)
Médula Ósea/patología , Glomerulonefritis/patología , Células Madre Hematopoyéticas/patología , Linfocitos/patología , Timo/patología , Diferenciación Celular , Enfermedad Crónica , ADN Nucleotidilexotransferasa/sangre , Femenino , Glomerulonefritis/sangre , Humanos , Linfocitos/metabolismo , Masculino , Factor Tímico Circulante/análisisRESUMEN
3H-thymidine incorporation was measured in DNA of the human lymphocytes from the peripheral blood after its PHA-stimulation in vitro. The increased PHA-stimulation was established after addition to the medium of exogenous terminal deoxynucleotidyl transferase (TDT) for cultures with small level of PHA-stimulation only. TDT without PHA exerted no stimulating action on the culture of lymphocytes.
Asunto(s)
ADN Nucleotidilexotransferasa/farmacología , ADN Nucleotidiltransferasas/farmacología , ADN/biosíntesis , Linfocitos/efectos de los fármacos , Fitohemaglutininas/farmacología , Células Cultivadas , Humanos , Linfocitos/metabolismo , Estimulación Química , Timidina/metabolismo , TritioAsunto(s)
Adenocarcinoma/radioterapia , Neoplasias de los Bronquios/radioterapia , Linfocitos/enzimología , Linfoma no Hodgkin/radioterapia , Adenocarcinoma/enzimología , Animales , Neoplasias de los Bronquios/enzimología , ADN de Neoplasias/metabolismo , Activación Enzimática/efectos de la radiación , Linfoma no Hodgkin/enzimología , Ratones , Ratones Endogámicos C57BL , Ratones Endogámicos CBA , Trasplante de Neoplasias , Neoplasias Experimentales/enzimología , Neoplasias Experimentales/radioterapia , Dosificación Radioterapéutica , RatasRESUMEN
The thymocyte's viability and hybridization of one fraction thymocyte RNA with exogenous DNA have been studied after the incubation of cells with this DNA. The exogenous DNA decreases the number of viable cells and increases the hybridization percentage of pulse-labeled RNA. The above changes failed to be induced after the incubation of these cells with the complex DNA with actinomycin D, despite the fact that this complex penetrates into the cells as easily as does the free DNA.
Asunto(s)
ADN/farmacología , Timo/efectos de los fármacos , Animales , Bovinos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , ADN/metabolismo , Dactinomicina/farmacología , Interacciones Farmacológicas , Técnicas In Vitro , Hibridación de Ácido Nucleico , Unión Proteica/efectos de los fármacos , Desnaturalización Proteica , ARN/metabolismo , Ratas , Timo/citología , Timo/efectos de la radiaciónRESUMEN
The number of defects of the secondary structure and dispersions of the molecular weights of DNA preparations were studied before and after their gamma-irradiation within a 5-2500 krad range of doses. These results are compared with the ability of the irradiated preparations to induce death of thymocytes irradiated in vivo during their combined incubation in vitro. It is established that the DNA biological activity is a result of the presence in the total preparation of molecule fractions of 5.10(6) or 1.10(6) Daltons with 3 or 50-80 defects of the secondary structure.
Asunto(s)
ADN/metabolismo , Timo/efectos de la radiación , Animales , Supervivencia Celular/efectos de la radiación , Relación Dosis-Respuesta en la Radiación , Técnicas In Vitro , Peso Molecular , Conformación de Ácido Nucleico , Bazo , Timo/metabolismoRESUMEN
The levels of total DNA, RNA and protein synthesis were examined in control and in vivo irradiated thymocytes after their preincubation with exogenous DNA of different polymerization degree. The studies were made using the uridine-14C and lysine-14C. The influence of preliminary treatment of exogenous DNA or cells with actinomycin D (AMD) was studied as applied to the levels of their own and "induced" synthesis in the cells. It is shown that native exogenous DNA induced the increase in the synthesis of all three components only in the irradiated cells. The pretreatment of the irradiated cells or exogenous DNA with AMD completely inhibits the "induced" synthesis of RNA and protein while their own synthesis in suppressed only by 20%. Possible transcription and translation of highly polymerized exogenous DNA in the irradiated cells without its integration into the genome of the "host" is discussed.
Asunto(s)
ADN/uso terapéutico , Traumatismos Experimentales por Radiación/tratamiento farmacológico , Linfocitos T/efectos de la radiación , Animales , ADN/biosíntesis , ADN/efectos de la radiación , Dactinomicina , Biosíntesis de Proteínas , Proteínas/efectos de la radiación , ARN/biosíntesis , ARN/efectos de la radiación , Ratas , Linfocitos T/metabolismo , Transcripción GenéticaRESUMEN
A relation is shown between the kinetic for of DNA uptake by the thymocytes (extremal or monotonous), the level of uptake (low or high) and the origin of DNA (hetero-or homologous). Preliminary irradiation of cells promotes a manifestation of a cytopathogenous effect of the heterologous DNA. The association between the cytopathogenous effect of DNA and that of its information characteristics has been confirmed. The demonstration of cytopathogenous effects of native and degraded DNA was studied as a function of time of their storage. The chromatographic analysis of dispersion of the molecular weights of preparations at different time of their starage allowed a conclusion of the important role played by structure defects in manifestation of cytopathogenous properties of DNA.
Asunto(s)
ADN/metabolismo , Genética de Radiación , Linfocitos T/metabolismo , Animales , Histocitoquímica , Técnicas In Vitro , Peso Molecular , RatasRESUMEN
The authors have studied in a comparative way the kinetics of incorporation of labelled heterologous DNA in tumor and nontumor cells of an identical histological type, obtained from non-irradiated and irradiated with 800 rad rats. It has been shown that the process of incorporation of heterologous high-polymer DNA in cells is somewhat undulatory, and its temporal characteristics and level -- tissue-specific. Difference between tumor cells and their normal prototype show a quantitative character, conditioned by a large surface area. 30 minutes following the irradiation qualitative changes opposite for both kinds of cells were observed in the kinetics of incorporation: an acceleration for lymphoyctes and retardation for Pliss lymphosarcoma and Zajdela hepatoma cells.
Asunto(s)
Carcinoma Hepatocelular/radioterapia , ADN/metabolismo , Neoplasias Hepáticas/radioterapia , Linfoma no Hodgkin/radioterapia , Animales , Transporte Biológico , Carcinoma Hepatocelular/metabolismo , Técnicas In Vitro , Neoplasias Hepáticas/metabolismo , Linfoma no Hodgkin/metabolismo , Neoplasias Experimentales/radioterapia , RatasRESUMEN
The uptake (or exclusion) of DNA into nuclei of the thymocytes in vitro is accompanied by decrease (or increase) in its resistance against exogenous pancreatic DNAse.