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The authors wish to make the following corrections to this paper [...].
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In this paper, we introduce a new family of distributions whose probability density function is defined as a weighted sum of two probability density functions; one is defined as a warped version of the other. We focus our attention on a special case based on the exponential distribution with three parameters, a dilation transformation and a weight with polynomial decay, leading to a new life-time distribution. The explicit expressions of the moments generating function, moments and quantile function of the proposed distribution are provided. For estimating the parameters, the method of maximum likelihood estimation is used. Two applications with practical data sets are given.
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Algoritmos , Modelos Estadísticos , Funciones de Verosimilitud , Distribuciones EstadísticasRESUMEN
Streptomyces are well-known antibiotic producers, also characterized by a complex morphological differentiation. Streptomyces, like all bacteria, are confronted with the constant threat of phage predation, which in turn shapes bacterial evolution. However, despite significant sequencing efforts recently, relatively few phages infecting Streptomyces have been characterized compared to other genera. Here, we present the isolation and characterization of five novel Streptomyces phages. All five phages belong to the Siphoviridae family, based on their morphology as determined by transmission electron microscopy. Genome sequencing and life style predictions suggested that four of them were temperate phages, while one had a lytic lifestyle. Moreover, one of the newly sequenced phages shows very little homology to already described phages, highlighting the still largely untapped viral diversity. Altogether, this study expands the number of characterized phages of Streptomyces and sheds light on phage evolution and phage-host dynamics in Streptomyces.
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Siphoviridae , Streptomyces coelicolor/virología , Streptomyces/virología , ADN Viral/genética , Genoma Viral , Especificidad del Huésped , Siphoviridae/clasificación , Siphoviridae/genética , Siphoviridae/aislamiento & purificaciónRESUMEN
We report here the draft genome sequence of Acinetobacter sp. Strain V2 isolated from the oil contaminated soil collected from ENGEN, Amanzimtoti, South Africa. Degradation of phenolic compounds such as phenol, toluene, aniline etc. at 400 ppm in 24 h and oil degrading capability makes this organism an efficient multifunctional bioremediator. Genome sequencing of Acinetobacter spp. V2 was carried out on Illumina HiSeq 2000 platform (performed by the Beijing Genomics Institute [BGI], Shenzhen, China). The data obtained revealed 643 contigs with genome size of 4.0 Mb and G + C content of 38.59%.(AU)
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Acinetobacter , Secuenciación de Nucleótidos de Alto Rendimiento , Análisis de Secuencia de ARN , Fenol , Sudáfrica , Contaminantes del SueloRESUMEN
Abstract We report here the draft genome sequence of Acinetobacter sp. Strain V2 isolated from the oil contaminated soil collected from ENGEN, Amanzimtoti, South Africa. Degradation of phenolic compounds such as phenol, toluene, aniline etc. at 400 ppm in 24 h and oil degrading capability makes this organism an efficient multifunctional bioremediator. Genome sequencing of Acinetobacter spp. V2 was carried out on Illumina HiSeq 2000 platform (performed by the Beijing Genomics Institute [BGI], Shenzhen, China). The data obtained revealed 643 contigs with genome size of 4.0 Mb and G + C content of 38.59%.
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Acinetobacter/genética , Acinetobacter/metabolismo , ADN Bacteriano/genética , ADN Bacteriano/química , Aceites/metabolismo , Genoma Bacteriano , Análisis de Secuencia de ADN , Fenoles/metabolismo , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , Sudáfrica , Composición de Base , Acinetobacter/aislamiento & purificación , BiotransformaciónRESUMEN
We report here the draft genome sequence of Acinetobacter sp. Strain V2 isolated from the oil contaminated soil collected from ENGEN, Amanzimtoti, South Africa. Degradation of phenolic compounds such as phenol, toluene, aniline etc. at 400ppm in 24h and oil degrading capability makes this organism an efficient multifunctional bioremediator. Genome sequencing of Acinetobacter spp. V2 was carried out on Illumina HiSeq 2000 platform (performed by the Beijing Genomics Institute [BGI], Shenzhen, China). The data obtained revealed 643 contigs with genome size of 4.0 Mb and G+C content of 38.59%.
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Acinetobacter/genética , Acinetobacter/metabolismo , ADN Bacteriano/química , ADN Bacteriano/genética , Genoma Bacteriano , Aceites/metabolismo , Análisis de Secuencia de ADN , Acinetobacter/aislamiento & purificación , Composición de Base , Biotransformación , Fenoles/metabolismo , Microbiología del Suelo , Contaminantes del Suelo/metabolismo , SudáfricaRESUMEN
ABSTRACT An efficient in vitro regeneration protocol for medicinally important herb Swertia chirayita was developed and the genetic fidelity was assessed using RAPD and ISSR markers. The best shoot regeneration was observed on MS basal supplemented with 1.0 mg/L Benzyl amino purine (BAP) in combination with Indole-3-acetic acid (IAA) (0.5 mg/L) that resulted in the increase by multiplication rate (7.65) with an average of 33.33 numbers of shoots and average shoot length of 2.70 cm. It was further enhanced by the addition of adenine sulfate (0.007%) that resulted in an average of 42 shoots per clum with 4.13 cm of average shoot length and the increase in multiplication fold to 9.75 that further resulted in the reduced use of other cytokinins and auxins. The rooting was nearly 100 % on 1/4 MS augmented with 1.0 mg/L Indole butyric acid with maximum average root length of 5.1cm. Plantlets were successfully acclimatized with 85-90 % survival rate. Ascorbate peroxidase activity increased with the maximum activity during the shoot multiplication. Clonal fidelity has been checked by two marker systems ISSR and RAPD and regenerated plants showed high clonal fidelity.