RESUMEN
Rodents are known to be reservoir hosts of Toxoplasma gondii infection for other animals, such as cats and pigs. From February to July 2017, 167 rats ( Rattus norvegicus) were trapped in Grenada, and serum, heart, skeletal muscle, and brain were examined for T. gondii infection by serological examination (modified agglutination test, 1:25) for T. gondii antibodies and for viable parasites by bioassay in mice. Samples of heart, skeletal muscle, and brain of all rats were bioassayed in Swiss Webster (SW) outbred albino mice and interferon gamma gene knockout (KO) mice. Toxoplasma gondii was isolated from heart and brain from 1 rat; this was the only seropositive rat. The T. gondii strain was avirulent for SW mice but killed KO mice. Tissue cysts were detected in the brains of SW mice, and tachyzoites were detected in the lungs of KO mice that died of acute toxoplasmosis. The strain was propagated in cell culture, and DNA derived from cell-cultured tachyzoites was genotyped using the 10 PCR restriction fragment length polymorphisms (SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico). The strain was a clonal Type III (ToxoDB genotype no. 2) strain. Although the prevalence of T. gondii in humans and animals in Grenada is high, rats seem to have little importance in the transmission of T. gondii on this island.
Asunto(s)
Toxoplasma/aislamiento & purificación , Toxoplasmosis Animal/epidemiología , Animales , Anticuerpos Antiprotozoarios/sangre , Bioensayo , Encéfalo/parasitología , Línea Celular , Femenino , Fibroblastos/parasitología , Marcadores Genéticos , Grenada/epidemiología , Corazón/parasitología , Humanos , Interferón gamma/genética , Pierna , Pulmón/parasitología , Pulmón/patología , Masculino , Ratones , Ratones Noqueados , Músculo Esquelético/parasitología , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Ratas , Toxoplasma/inmunologíaRESUMEN
Rodents are intermediate hosts for many species of Sarcocystis. Little is known of Sarcocystis cymruensis that uses the Brown rat (Rattus norvegicus) as intermediate hosts and the domestic cat (Felis catus) as experimental definitive host. Here, we identified and described Sarcocystis cymruensis in naturally infected R. norvegicus from Grenada, West Indies. Rats (n = 167) were trapped in various locations in two parishes (St. George and St. David). Microscopic, thin (< 1 µm) walled, slender sarcocysts were found in 11 of 156 (7.0%) rats skeletal muscles by squash examination. A laboratory-raised cat fed naturally infected rat tissues excreted sporocysts that were infectious for interferon gamma gene knockout (KO) mice, but not to Swiss Webster outbred albino mice. All inoculated mice remained asymptomatic, and microscopic S. cymruensis-like sarcocysts were found in the muscles of KO mice euthanized on day 70, 116, and 189 post inoculation (p.i.). Sarcocysts from infected KO mice were infective for cats at day 116 but not at 70 days p.i. By transmission electron microscopy, the sarcocyst wall was "type 1a." Detailed morphological description of the cyst wall, metrocytes, and bradyzoites is given for the first time. Additionally, molecular data on S. cymruensis are presented also for the first time. Molecular characterization of sarcocysts 18S rDNA and 28S rDNA, ITS-1, and cox1 loci showed the highest similarity with S. rodentifelis and S. muris. In conclusion, the present study described the natural infection of S. cymruensis in Brown rat for the first time in a Caribbean country and provided its molecular characteristics.
Asunto(s)
Interferón gamma/genética , Músculos/parasitología , Oocistos/aislamiento & purificación , Sarcocystis/genética , Sarcocystis/aislamiento & purificación , Sarcocistosis/veterinaria , Animales , Gatos , ADN Intergénico/genética , Grenada , Ratones , Ratones Noqueados , Microscopía Electrónica de Transmisión , ARN Ribosómico 18S/genética , ARN Ribosómico 28S/genética , Ratas , Sarcocystis/clasificaciónRESUMEN
Gastrointestinal parasites are important in small ruminant farming because they can impact negatively on the productivity of animals. The objectives of the present study were to estimate the prevalence of gastrointestinal parasites and to assess mortality attributable to gastrointestinal parasite infection in sheep and goats. We collected fecal samples from 114 sheep and 292 goats from 34 farms for coprological examination. In addition, we evaluated necropsy records for sheep and goats that were submitted from 2002 to 2016 to the pathology diagnostic laboratory in the School of Veterinary Medicine at St. George's University, Grenada. Out of 406 small ruminant (292 goat and 114 sheep) fecal samples examined, 385 were positive for gastrointestinal parasites, giving an overall prevalence of 95% (95% confidence interval (CI) 92% to 97%). All the 34 farms visited were found to have positive animals to at least one type of gastrointestinal parasite; 100% herd prevalence (95% CI: 88% to 100%). Among the 292 goat fecal samples examined, 285 were positive for gastrointestinal parasites (98%; 95% CI: 95% to 99%) whereas the proportion of positive fecal samples in sheep was 88% (95% CI: 80% to 93%). There was a significant difference in prevalence of gastrointestinal parasites between sheep and goats (pâ¯=â¯.0002). The proportion of infection with coccidia in goats and sheep was 76% and 75%, respectively. For helminthes, the proportions were as follows: Moniezia spp., 14% in goats and 4% in sheep; Strongyloides spp., 36% in goats and 21% in sheep; strongyle type eggs 89% in goats and 66% in sheep. Mixed infections in both sheep and goats were more common (92%) than single ones (8%). Out of 220 necropsy records evaluated, 29% of mortality was due to Haemonchus contortus infection. Moniezia spp and Oesophagostomum spp. were also commonly found.
Asunto(s)
Parasitosis Intestinales/veterinaria , Parásitos/aislamiento & purificación , Enfermedades Parasitarias en Animales/epidemiología , Rumiantes/parasitología , Animales , Autopsia , Granjas , Heces/parasitología , Enfermedades de las Cabras/epidemiología , Enfermedades de las Cabras/parasitología , Cabras , Grenada/epidemiología , Parasitosis Intestinales/epidemiología , Ganado/parasitología , Recuento de Huevos de Parásitos , Parásitos/clasificación , Prevalencia , Ovinos , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/parasitología , Encuestas y CuestionariosRESUMEN
The objectives of the present cross-sectional study were to isolate and genotype Toxoplasma gondii in free-range chickens from Grenada, West Indies. Using the modified agglutination test, antibodies to T. gondii were found in 39 (26.9%) of 145 free-range chickens with titers of 25 in 7 chickens, 50 in 6 chickens, 100 in 2 chickens, and 200 or higher in 24 chickens. The hearts of the 39 seropositive chickens were bioassayed in mice; viable T. gondii was isolated from 20 and further propagated in cell culture. Genotyping of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed 4 genotypes, including ToxoDB PCR-RFLP no. 2 (Type III), no. 7, no. 13, and no. 259 (new). These results indicated that T. gondii population genetics in free-range chickens seems to be moderately diverse with ToxoDB no. 2 (Type III) as the most frequent (15/20 = 75%) compared to other genotypes in Grenada.
Asunto(s)
Pollos/parasitología , Técnicas de Genotipaje/veterinaria , Polimorfismo de Longitud del Fragmento de Restricción , Enfermedades de las Aves de Corral/parasitología , Toxoplasma/aislamiento & purificación , Toxoplasmosis Animal/parasitología , Pruebas de Aglutinación/veterinaria , Animales , Anticuerpos Antiprotozoarios/sangre , Bioensayo/veterinaria , Estudios Transversales , Genotipo , Grenada/epidemiología , Corazón/parasitología , Ratones , Reacción en Cadena de la Polimerasa , Enfermedades de las Aves de Corral/epidemiología , Estudios Seroepidemiológicos , Toxoplasma/clasificación , Toxoplasma/genética , Toxoplasma/inmunología , Toxoplasmosis Animal/epidemiologíaRESUMEN
Over a 2-year period 66 cases of canine pyoderma in Grenada, West Indies, were examined by aerobic culture in order to ascertain the bacteria involved and their antimicrobial resistance patterns. Of the 116 total bacterial isolates obtained, the majority belonged to Gram-positive species, and the most common organism identified through biochemical and molecular methods was Staphylococcus pseudintermedius. Additionally, identification of a Staphylococcus schleiferi subspecies coagulans isolate was confirmed by molecular methods. All isolates of staphylococci were susceptible to beta-lactam drugs: amoxicillin-clavulanic acid, cefovecin, cefoxitin, cefpodoxime, and cephalothin. They were also susceptible to chloramphenicol and enrofloxacin. Resistance was highest to tetracycline. Methicillin resistance was not detected in any isolate of S. pseudintermedius or in S. schleiferi. Among the Gram-negative bacteria, the most common species was Klebsiella pneumoniae, followed by Acinetobacter baumannii/calcoaceticus. The only drug to which all Gram-negative isolates were susceptible was enrofloxacin. This report is the first to confirm the presence of S. pseudintermedius and S. schleiferi subspecies coagulans, in dogs with pyoderma in Grenada, and the susceptibility of staphylococcal isolates to the majority of beta-lactam drugs used in veterinary practice.
RESUMEN
Surveillance for Mycobacterium avium subsp. paratuberculosis (Map) infection in small ruminants of Grenada was undertaken using a commercial enzyme-linked immunosorbent assay (ELISA). Among the 479 sheep tested, 11 (2.3%) were ELISA positive while only 1 out of 260 goats (0.3%) was ELISA positive. Five of the 12 ELISA-positive animals were also positive in a commercial agar gel immunodiffusion (AGID) assay, and 4 of these showed acid-fast rods consistent with Map in fecal smears. Two sheep that were test-positive by ELISA, AGID, and fecal smears were euthanized and necropsied. Both had gross and histological lesions of paratuberculosis affecting the ileocecal area of small intestines and adjacent lymph nodes. These tissues were successfully cultured in 2 of 3 variants of Middlebrook 7H10 medium. The identity of acid-fast organisms isolated from the tissues was confirmed as Map by multiplex conventional polymerase chain reaction. Using IS1311 amplification and Hinf I restriction digest analysis, isolates were identified as cattle (C) strains of Map. The current study describes Map infection in Grenada and confirms the presence of C type in sheep on the island of Carriacou. The low seroprevalence in clinically normal animals on the islands of Grenada and Carriacou suggests that control measures implemented in the near future may have a good chance of preventing spread of the infection.
Asunto(s)
Enfermedades de las Cabras/microbiología , Enfermedades Intestinales/veterinaria , Mycobacterium avium subsp. paratuberculosis/aislamiento & purificación , Paratuberculosis/microbiología , Enfermedades de las Ovejas/microbiología , Animales , Anticuerpos Antibacterianos/sangre , ADN Bacteriano/química , ADN Bacteriano/genética , Ensayo de Inmunoadsorción Enzimática/veterinaria , Enfermedades de las Cabras/epidemiología , Cabras , Grenada/epidemiología , Enfermedades Intestinales/epidemiología , Enfermedades Intestinales/microbiología , Paratuberculosis/epidemiología , Reacción en Cadena de la Polimerasa/veterinaria , Estudios Seroepidemiológicos , Ovinos , Enfermedades de las Ovejas/epidemiologíaRESUMEN
Little is known of the genetic diversity and epidemiology of Toxoplasma gondii infection in wildlife in Caribbean Islands. The prevalence and genetic diversity of T. gondii in mongooses (Herpestes auropunctatus) was investigated. During 2011 and 2012, 91 mongooses were trapped in different parts of Grenada, bled, euthanized, and examined at necropsy. Antibodies to T. gondii were found in 27 mongooses tested by the modified agglutination test (cut-off titer 25). Muscles (heart, tongue, neck) of 25 of the seropositive mongooses were bioassayed for T. gondii infection in mice. Viable T. gondii was isolated by bioassay in mice from four mongooses with MAT titers of 1:50 in two, 1:200 for one, and 1:400 for one mongoose. The four T. gondii isolates were further propagated in cell culture. Strain typing of T. gondii DNA extracted from cell-cultured tachyzoites using the 10 PCR-restriction fragment length polymorphism (RFLP) markers SAG1, SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed one isolate belongs to the Type III (ToxoDB #2) lineage, two to ToxoDB#7 lineage, and one to the ToxoDB #216 lineage. This is the first report of T. gondii isolation and genotyping in H. auropunctatus worldwide.
Asunto(s)
Herpestidae/parasitología , Toxoplasma/genética , Toxoplasmosis Animal/parasitología , Animales , Genotipo , Polimorfismo de Longitud del Fragmento de Restricción , Toxoplasma/aislamiento & purificación , Toxoplasmosis Animal/epidemiología , Indias Occidentales/epidemiologíaRESUMEN
In a 2-year period 54 feral cats were captured in Grenada, West Indies, and a total of 383 samples consisting of swabs from rectum, vagina, ears, eyes, mouth, nose and wounds/abscesses, were cultured for aerobic bacteria and campylobacters. A total of 251 bacterial isolates were obtained, of which 205 were identified to species level and 46 to genus level. A commercial bacterial identification system (API/Biomerieux), was used for this purpose. The most common species was Escherichia coli (N=60), followed by Staphylococcus felis/simulans (40), S. hominis (16), S. haemolyticus (12), Streptococcus canis (9), Proteus mirabilis (8), Pasteurella multocida (7), Streptococcus mitis (7), Staphylococcus xylosus (7), S. capitis (6), S. chromogenes (4), S. sciuri (3), S. auricularis (2), S. lentus (2), S. hyicus (2), Streptococcus suis (2) and Pseudomonas argentinensis (2). Sixteen other isolates were identified to species level. A molecular method using 16S rRNA sequencing was used to confirm/identify 22 isolates. Salmonella or campylobacters were not isolated from rectal swabs. E. coli and S. felis/simulans together constituted 50% of isolates from vagina. S. felis/simulans was the most common species from culture positive ear and eye samples. P. multocida was isolated from 15% of mouth samples. Coagulase-negative staphylococci were the most common isolates from nose and wound swabs. Staphylococcus aureus, or S. intemedius/S. pseudintermedius were not isolated from any sample. Antimicrobial drug resistance was minimal, most isolates being susceptible to all drugs tested against, including tetracycline.
Asunto(s)
Antibacterianos/farmacología , Bacterias Aerobias/efectos de los fármacos , Infecciones Bacterianas/veterinaria , Enfermedades de los Gatos/microbiología , Conducto Auditivo Externo/microbiología , Membrana Mucosa/microbiología , Heridas y Lesiones/microbiología , Animales , Bacterias Aerobias/clasificación , Bacterias Aerobias/aislamiento & purificación , Infecciones Bacterianas/microbiología , Gatos , Ojo/microbiología , Femenino , Grenada , Pruebas de Sensibilidad Microbiana , Recto/microbiología , Piel/lesiones , Piel/microbiología , Vagina/microbiologíaRESUMEN
In Grenada, backyard flocks and unorganized poultry farms contribute to the major share of table eggs and moreover regulations for processing of eggs before retail sale are not at all enforced. To evaluate the bacterial contamination of table eggs, a total of 450 eggs collected from different sources including small farms (150 eggs), large farms (120 eggs), road side vendors (120 eggs) and supermarkets (60 eggs), were cultured for the bacteria. A total of 226 bacterial cultures predominantly of the family Enterobacteriaceae were obtained with a higher proportion from shell membrane (54.4%) than from yolk samples (45.6%). There was no significant difference (P< 0.05) in the number of isolates between various sources indicating the contamination at farm level. The study indicates the need for optimum hygienic conditions at the farm level to decrease the bacterial load.
Asunto(s)
Embrión de Pollo , Infecciones Bacterianas , Pollos , Huevos , GrenadaRESUMEN
In Grenada, West Indies dogs are at frequent exposure to the rickettsial pathogen, Ehrlichia canis, as demonstrated by high seroprevalence rates. However, many of these seropositive dogs are clinically normal. In this study we identified clinically normal, E. canis seropositive dogs and assigned half to an antibiotic treatment group and half to a no treatment group. All dogs were evaluated for the presence of E. canis DNA by PCR on whole blood before, during and after treatment. Only one seropositive dog was also PCR+ before treatment. Our results suggest that most clinically normal, E. canis seropositive dogs in a highly endemic geographic area are not concurrently infected and thus routine treatment of clinically normal, seropositive dogs is not warranted.
Asunto(s)
Perros , Ehrlichia canis , Perros , Serología , Reacción en Cadena de la Polimerasa/veterinaria , GrenadaRESUMEN
The pilot study determines the potential role of bacterial contamination of egg surfaces at the time of oviposition and in the sand of the nesting chambers in lowering hatchability. A total of 15 species of bacteria were isolated from 20 eggs and 17 sand samples of egg nests, with little overlap in the species spectrum between eggs and sand. The most frequent bacteria found on the egg surface were Pseudomonas spp. followed by Citrobacter spp., Enterobacter cloacae and Morganella morganii, whereas from sand samples most frequent isolates were Bacillus spp. followed by Enterobacter spp. and Pseudomonas spp. All 15 isolated species are considered opportunistic pathogens, and could be potential causes for the reported lower hatchability. These pathogens also constitute a public health risk when eggs are consumed by humans. The majority of isolates showed antibiotic drug resistance, indicating environmental pollution.