RESUMEN
A human DNA library has been searched for moderate repetitive sequences. Southern's blot analysis of one of the selected clones, which we have termed pTURI-6, has shown a high interindividual restriction fragment length and copy polymorphism. TURI-6 clone can serve as an efficient molecular marker for the identification of interindividual distinctions.
Asunto(s)
ADN/genética , Familia de Multigenes , Polimorfismo Genético , Polimorfismo de Longitud del Fragmento de Restricción , Secuencias Repetitivas de Ácidos Nucleicos , Clonación Molecular , ADN Recombinante , Diploidia , Humanos , Hibridación de Ácido NucleicoRESUMEN
Immunologic changes in schizophrenic psychoses were examined by different methods of genetic analysis. The cytotoxic test and the method of fluorescent antibodies showed an increase in the antithymocytic activity (ATA) of the blood serum. A high index of the coefficient of a genetic correlation between elevated blood levels of ATA and the genetic component of liability to the disease indicates that genetic factors partially involved in the general structure of hereditary predisposition to schizophrenic psychoses participate in the determination of interindividual differences with regard to blood ATA levels. The data obtained suggest that the observed antithymocytic activity of the blood serum was due to at least 2 types of antibodies with different affinity to thymocytes.
Asunto(s)
Suero Antilinfocítico , Esquizofrenia/genética , Linfocitos T/inmunología , Adulto , Pruebas Inmunológicas de Citotoxicidad , Enfermedades en Gemelos , Femenino , Técnica del Anticuerpo Fluorescente , Marcadores Genéticos , Humanos , Masculino , Persona de Mediana Edad , Embarazo , Riesgo , Gemelos Dicigóticos , Gemelos MonocigóticosRESUMEN
Nucleotide sequencing of two terminal subfragments of a cloned human DNA fragment has been done. The fragment cloned hybridized dispersively along all chromosomes except for Y chromosome and C heterochromatic chromosome regions. Both subfragments sequenced contain Alu repeats, whose structures differ partially from those of accurately determined sequences of human Alu repeats. The results obtained are discussed in respect of possible usage of Alu repeats containing sequences for construction of special polymorphic molecular markers of human chromosomes.
Asunto(s)
Cromosomas Humanos/análisis , ADN/genética , Secuencia de Bases , ADN/análisis , ADN Recombinante , Genes , Marcadores Genéticos , Humanos , Linfocitos/ultraestructura , Metafase , Hibridación de Ácido Nucleico , Plásmidos , Secuencias Repetitivas de Ácidos NucleicosRESUMEN
A genetic analysis of the immunological disturbances observed in patients with schizophrenic psychoses and registered from the level of the serum antilymphocytic activity was carried out. The analysis has shown that the contribution of genetic factors to the determination of interindividual differences in that feature exceeds more than twice the contribution of environmental factors. The close correlation between the serum antilymphocytic activity and the genetic component of the predisposition to the disease gives one grounds to regard a rise of the serum antilymphocytic activity as a pathogenetic marker of hereditary predisposition to schizophrenia. The results obtained, if taken together with literary data, may be of interest for studies concerned with the multicomponent structure of the hereditary predisposition to schizophrenic psychoses and their pathogenesis.
Asunto(s)
Suero Antilinfocítico , Enfermedades en Gemelos , Esquizofrenia/genética , Pruebas Inmunológicas de Citotoxicidad , Femenino , Marcadores Genéticos , Humanos , Embarazo , Esquizofrenia/inmunología , Gemelos Dicigóticos , Gemelos MonocigóticosRESUMEN
Ontogenetic changes of normal diploid cells and cells with genetic mutations (LHC-484 from a patient with xeroderma pigmentosum) have been studied under prolonged cultivation in a stationary phase. While investigating the dynamics of complex changes in certain cell properties and cell populations it was found that the cultivated human cells possess a particular pattern of ontogenetic changes for every culture and the dynamics of these changes depends upon the density of the cell population. Two cell subpopulations different in the degree of nucleus heterochromotinization have been discovered.