RESUMEN
Background: The effects of rosmarinic acid (RA) on immunological and inflammatory mediator levels in bronchoalveolar lavage fluid (BALF) as well as lung pathological changes in asthmatic rats were investigated. Methods: The levels of IFN-gama, IL-4, IFN-gama/IL-4 ratio, IgE, PLA2, and total protein (TP) in BALF and pathological changes in the lung were evaluated in control group (C), asthma group (sensitized to ovalbumin) (A), asthma groups treated with RA and dexamethasone. Results: Compared to the control group, asthmatic rats showed increased levels of IL-4, IgE, PLA2, and TP as well as all pathological scores with decreased levels of IFN-gama and IFN-gama/IL-4 ratio (P < 0.05 to P < 0.001). The levels of IL-4, IgE, PLA2, and TP significantly reduced in groups treated with all concentrations of RA compared to asthma group (P < 0.001 for all cases). IFN-gama was significantly decreased in groups treated with two lower concentrations of RA but IFN-gama/IL-4 ratio was increased in groups treated with two higher concentrations of RA compared to asthma group (P<0.05 to P < 0.001). Treatment with all doses of RA led to significant improvement in pathological scores in asthmatic animals (P < 0.05 to P < 0.001). Most measured parameters were also significantly improved in dexamethasone-treated animals (P<0.01 to P < 0.001) but IFN-gama/IL-4 ratio and the scores of interstitial fibrosis, bleeding and epithelial damage did not change in this group. Conclusion: The results indicated a preventive effect for RA on immunological and inflammatory mediators as well as lung pathological changes in asthmatic rats which were comparable or even more potent than that of dexamethasone
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Asunto(s)
Humanos , Animales , Masculino , Ratas , Antiinflamatorios/uso terapéutico , Asma/tratamiento farmacológico , Cinamatos/uso terapéutico , Depsidos/uso terapéutico , Alérgenos/inmunología , Citocinas/sangre , Dexametasona/uso terapéutico , Modelos Animales de Enfermedad , Inmunoglobulina E/sangre , Mediadores de Inflamación/sangre , Ovalbúmina/inmunología , Ratas WistarRESUMEN
BACKGROUND: The effects of rosmarinic acid (RA) on immunological and inflammatory mediator levels in bronchoalveolar lavage fluid (BALF) as well as lung pathological changes in asthmatic rats were investigated. METHODS: The levels of IFN-γ, IL-4, IFN-γ/IL-4 ratio, IgE, PLA2, and total protein (TP) in BALF and pathological changes in the lung were evaluated in control group (C), asthma group (sensitized to ovalbumin) (A), asthma groups treated with RA and dexamethasone. RESULTS: Compared to the control group, asthmatic rats showed increased levels of IL-4, IgE, PLA2, and TP as well as all pathological scores with decreased levels of IFN-γ and IFN-γ/IL-4 ratio (P<0.05 to P<0.001). The levels of IL-4, IgE, PLA2, and TP significantly reduced in groups treated with all concentrations of RA compared to asthma group (P<0.001 for all cases). IFN-γ was significantly decreased in groups treated with two lower concentrations of RA but IFN-γ/IL-4 ratio was increased in groups treated with two higher concentrations of RA compared to asthma group (P<0.05 to P<0.001). Treatment with all doses of RA led to significant improvement in pathological scores in asthmatic animals (P<0.05 to P<0.001). Most measured parameters were also significantly improved in dexamethasone-treated animals (P<0.01 to P<0.001) but IFN-γ/IL-4 ratio and the scores of interstitial fibrosis, bleeding and epithelial damage did not change in this group. CONCLUSION: The results indicated a preventive effect for RA on immunological and inflammatory mediators as well as lung pathological changes in asthmatic rats which were comparable or even more potent than that of dexamethasone.
Asunto(s)
Antiinflamatorios/uso terapéutico , Asma/tratamiento farmacológico , Cinamatos/uso terapéutico , Depsidos/uso terapéutico , Alérgenos/inmunología , Animales , Citocinas/sangre , Dexametasona/uso terapéutico , Modelos Animales de Enfermedad , Humanos , Inmunoglobulina E/sangre , Mediadores de Inflamación/sangre , Masculino , Ovalbúmina/inmunología , Ratas , Ratas Wistar , Ácido RosmarínicoRESUMEN
OBJECTIVE: Platelet-derived growth factor (PDGF-BB) is an important factor in the regeneration and wound healing. One of the problems is that there is not enough efficiency in the transmission or delivery of such factors in the wound site. In this study, alginate sulfate hydrogel was synthesized with recombinant PDGF-BB growth factor for achieving a quick method in wound healing. METHODS: In this study, Alginate sulfate hydrogel was made by photo-crosslinking method and methacrylate. Its toxicity was evaluated by MTT assay. Transforming growth factor was studied in releasing from synthesized alginate sulfate hydrogels and also, lack of toxicity was confirmed, and hydrogel was made with a recombinant human growth factor. Wounds were created with a diameter of 10 mm on the back of rats in order to check the wound healing. RESULTS: This study showed that alginate sulfate hydrogels-PDGF-BB were faster in wound healing than non-sulfate alginate hydrogels-PDGF-BB. Therefore, the controlled delivery of growth factor system can be a powerful idea for therapeutic applications for wound healing. CONCLUSION: Alginate sulfate hydrogel with recombinant growth factor 4 µg/cm2 (PDGF-BB) was very suitable for wound healing (Tab. 1, Fig. 3, Ref. 34).
Asunto(s)
Alginatos/farmacología , Inductores de la Angiogénesis/farmacología , Hidrogeles/farmacología , Proteínas Proto-Oncogénicas c-sis/farmacología , Proteínas Recombinantes/farmacología , Sulfatos/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Becaplermina , Ratas , Regeneración/efectos de los fármacosRESUMEN
Incheh Broun hypersaline wetland is located near the border of Turkmenistan in thenorth of Iran. This wetland is notable because of salinity (280g/l) and alteration in pH range (2.8 to 6.8). Eastern part of wetland is affected by wastewater of iodine extraction factory. Samples were taken from soil, water and salt. Totally, 400 bacterial strains were isolated of which 194 strains were Gram-positive bacilli, 184 strains were Gram-negative rod and 22 strains were Gram-positive cocci. According to phylogenetic analysis of 16S rRNA, selected strains were placed in three taxonomic phyla including Firmicutes, Actinobacteria and Gammaproteobacteria. Optimum growth was evaluated for salt and 22 strains were found to be moderate halophile and 33 strains were halotolerant. Production of lipase, amylase, gelatinase and protease was examined. Gram-positive bacilli were the main producers of hydrolytic enzymes. Gelatinase and protease were the most frequent enzymes. Gram-positive cocci were the main producers of lipase but they didn't produce amylase.
Asunto(s)
Bacterias/genética , Microbiología del Suelo , Bacterias/clasificación , Bacterias/aislamiento & purificación , ADN Bacteriano/aislamiento & purificación , ADN Bacteriano/metabolismo , Bacterias Gramnegativas/clasificación , Bacterias Gramnegativas/genética , Bacterias Gramnegativas/aislamiento & purificación , Bacterias Grampositivas/clasificación , Bacterias Grampositivas/genética , Bacterias Grampositivas/aislamiento & purificación , Concentración de Iones de Hidrógeno , Irán , Filogenia , ARN Ribosómico 16S/química , ARN Ribosómico 16S/genética , ARN Ribosómico 16S/metabolismo , Salinidad , Análisis de Secuencia de ADN , Eliminación de Residuos Líquidos , HumedalesRESUMEN
The PDGF-BB plays a key role in several pathogenesis diseases and it is believed to be an important mediator for wound healing. The recombinant human PDGF-BB is safe and effective to stimulate the healing of chronic, full thickness and lower extremity diabetic neurotrophic ulcers. In the present study, we attempted to produce a PDGF-BB growth factor and also, evaluate its functionality in cell proliferation in yeast host Pichia pink. Pichia pink yeast was used as a host for evaluation of the rhPDGF-BB expression. The coding sequence of PDGF-BB protein was synthesized after optimization and packed into the pGEM. Recombinant proteins were produced and purified. The construct of pPinkα-HC-pdgf was confirmed by sequence, the PDGF-BB protein was expressed and purified with using a nickel affinity chromatography column and then characterized by SDS-PAGE electrophoresis. The biological activity of PDGF-BB was estimated with using human fibroblast cell line. The measurement of protein concentration was determined by Bradford and human PDGF-BB ELISA kit. Purified rhPDGF-BB showed similar biological activity (as the standard PDGF-BB) and suggested that the recombinant protein has a successful protein expression (as well as considerable biological activity in P. pink host). The exact amount of recombinant PDGF-BB concentrations were measured by specific ELISA test which it was about 30 µg/ml. Our study suggested that efficiency of biological activity of PDGF-BB protein may be related to its conformational similarity with standard type and also, it practically may be important in wound healing and tissue regeneration.
Asunto(s)
Pichia/metabolismo , Proteínas Proto-Oncogénicas c-sis/genética , Proteínas Recombinantes/genética , Células 3T3 , Animales , Becaplermina , Western Blotting , Clonación Molecular , Electroforesis en Gel de Poliacrilamida , Humanos , Ratones , Plásmidos/metabolismo , Reacción en Cadena de la Polimerasa , Proteínas Proto-Oncogénicas c-sis/aislamiento & purificación , Proteínas Proto-Oncogénicas c-sis/metabolismo , Proteínas Recombinantes/aislamiento & purificación , Estándares de Referencia , Reproducibilidad de los Resultados , Transformación GenéticaRESUMEN
OBJECTIVES: Menopausal women are interested in alternative therapy to alleviate climacteric symptoms. This study aimed to investigate the effect of red clover on the severity of menopausal symptoms. METHODS: This randomized, placebo-controlled, clinical trial included 72 healthy postmenopausal women, who were randomly divided into intervention and placebo groups. Women in the intervention group received two capsules containing 40 mg dried leaves of red clover daily for 12 weeks, while those in the control group received two capsules containing 40 mg starch daily for 12 weeks. Outcome measures of this study were menopausal symptoms determined using the Menopause Rating Scale (MRS) at baseline and at the end of the study. Sociodemographic data of the included women were collected using a questionnaire. RESULTS: No significant differences were observed between the two groups with respect to the demographic characteristics and menopausal symptoms before the intervention. At the end of the study, the total score on the MRS decreased from 20.41 to 10.08 in the intervention group and from 20.77 to 17.20 in the control group. This decrease in the total score was attributed to the scores of vegetative somatic and psychological categories of menopausal symptoms. Thus, a remarkable difference was observed in the severity of the menopausal symptoms (95% confidence interval, p = 0.0001). CONCLUSION: These results indicated that, compared to placebo, dried leaves of red clover were more effective in reducing the severity of menopausal symptoms in postmenopausal women.