RESUMEN
The effect of N-palmitoylethanolamine (NPE, 10(-5) M) on the lipid peroxidation (LPO) processes, phospholipid and fatty acid content in the rat liver at perfusion and ischemia during the liver preservation was estimated. As early as at the 5th min of perfusion by cooled conserving solution "Eurocollins", LPO activation was determined. Simultaneously the content of lysophosphatidylcholine (LPC) and phosphatidylserine increased, the total cholesterol level decreased. The redistribution of saturated and unsaturated fatty acids quantity was detected. The addition of NPE into "Eurocollins" solution reduced the accumulation of malonedialdehyde and LPC, modified the fatty acids content. These effects, evidently, formed the basis of the protective action of NPE on the hepatic tissues under anoxia.
Asunto(s)
Ácidos Grasos/metabolismo , Isquemia/metabolismo , Hígado/efectos de los fármacos , Ácidos Palmíticos/farmacología , Fosfolípidos/metabolismo , Amidas , Animales , Endocannabinoides , Etanolaminas , Peroxidación de Lípido , Hígado/irrigación sanguínea , Hígado/metabolismo , Masculino , Malondialdehído/metabolismo , RatasRESUMEN
The effect of 10(-5) M N-palmitoylethanolamine (NPE) on lipid peroxidation processes, phospholipid and fatty acid content in the ischemic rat liver under reperfusion was estimated. It was shown the significant accumulation of thiobarbituric acid reactive substances (TBARS), the increase of lysophosphatidylethanolamine (LPC), phosphatidylserine, phosphatidylethanolamine, sphingomyelin, general cholesterol content and also the decrease of phosphatidylcholine amount in the ischemic rat liver under reperfusion. Simultaneously the redistribution of saturated and unsaturated fatty acids quantity was detected, that indicates the increasing of membrane rigidity. The addition of NPE into the Eurocollins and Bretshneider's solutions under perfusion and preservation of a donor organ reduced the accumulation of TBARS, LPC, total cholesterol, prevented the changes of cholesterol/phospholipids and saturated/unsaturated fatty acids ratio value in the same experimental conditions. These effects, evidently, formed the basis of the protective action of NPE on the ischemic liver tissues under reperfusion.
Asunto(s)
Ácidos Grasos/metabolismo , Hígado/irrigación sanguínea , Ácidos Palmíticos/farmacología , Fosfolípidos/metabolismo , Daño por Reperfusión/metabolismo , Amidas , Animales , Endocannabinoides , Etanolaminas , Peroxidación de Lípido , Masculino , Ratas , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
Preservation of hepar of 86 rats using Euro-Collins' and Bretschneider preservation solutions was realized in experiment. The animals hepar anatomy was studied thoroughly, the hepar preservation pattern was improved, the modified pattern of the hepar isolated perfusion was used. Morphologic, cytologic and biochemical investigations were conducted to estimate the viability of hepar.
Asunto(s)
Hígado/anatomía & histología , Hígado/cirugía , Preservación de Órganos/métodos , Reperfusión/métodos , Animales , Femenino , Glucosa , Arteria Hepática/anatomía & histología , Arteria Hepática/cirugía , Venas Hepáticas/anatomía & histología , Venas Hepáticas/cirugía , Soluciones Hipertónicas , Masculino , Manitol , Soluciones Preservantes de Órganos , Cloruro de Potasio , Procaína , Ratas , Factores de Tiempo , TrometaminaRESUMEN
The influence of Euro-Collins and Euro-Collins with the membrane stabilizing additives conserving solutions on the course of dystrophic process in the rat's hepatic parenchyma was studied. Application of the membrane stabilizing additives promoted the hepatic structure stabilization, showing the reduce of the hydropic dystrophia intensity and extent.