RESUMEN
The present study was designed to investigate the mineral content and antimicrobial activity of Curcuma Longa extracts and its essential oil. We also determined the lipid peroxidation inhibition activity of the ethanolic extract against sodium nitroprusside (SNP) induced thiobarbituric acid reactive species (TBARS) formation in rat's brain, kidney and liver homogenates. Major constituents of essential oil identified by gas chromatography and mass spectrometry (GCMS) were beta-sesquiphellandrene (38.69%), alpha-curcumene (18.44%) and p-mentha-1,4 (8)-diene (16.29%). Atomic absorption spectroscopy (AAS) was used for the quantitative estimation of Calcium (Ca), Magnesium (Mg), Iron (Fe), Copper (Cu), Zinc (Zn), Chromium (Cr), Nickel (Ni) and Manganese (Mn). The extract showed highest Mg (49.4 mg/l) concentration followed by Ca (35.42 mg/l) and Fe (1.27 mg/l). Our data revealed that the ethanolic extract of Curcuma Longa at 1-10 mg/kg significantly inhibited TBARS production in all tested homogenates. Crude extracts and essential oil were tested against three gram positive bacteria i.e. Bacillus subtilis, Bacillus atrophoeus, Staphylococcus aureus, six gram negative bacteria i.e. Escherichia coli, Klebsiella pneumonias, Salmonella typhi, Pseudomonas aeruginosa, Erwinia carotovora, Agrobacterium tumefaciens and one fungal strain namely Candida albicans by disc diffusion assay. Essential oil showed highest anti-microbial activity as compared to the crude extracts. The present study confirms the significant antimicrobial and antioxidant potential of the studied plant, which can be considered as a diet supplement for a variety of oxidative stress induced or infectious diseases.
Asunto(s)
Antiinfecciosos/farmacología , Antioxidantes/farmacología , Curcuma/química , Cromatografía de Gases y Espectrometría de Masas , Peroxidación de Lípido/efectos de los fármacos , Aceites Volátiles/farmacología , Estrés Oxidativo/efectos de los fármacos , Extractos Vegetales/farmacología , Aceites de Plantas/farmacología , Antiinfecciosos/aislamiento & purificación , Antioxidantes/aislamiento & purificación , Bacterias/efectos de los fármacos , Bacterias/crecimiento & desarrollo , Encéfalo/efectos de los fármacos , Encéfalo/metabolismo , Candida albicans/efectos de los fármacos , Candida albicans/crecimiento & desarrollo , Pruebas Antimicrobianas de Difusión por Disco , Relación Dosis-Respuesta a Droga , Etanol/química , Riñón/efectos de los fármacos , Riñón/metabolismo , Hígado/efectos de los fármacos , Hígado/metabolismo , Malondialdehído/metabolismo , Nitroprusiato/farmacología , Aceites Volátiles/aislamiento & purificación , Fitoterapia , Extractos Vegetales/aislamiento & purificación , Aceites de Plantas/aislamiento & purificación , Plantas Medicinales , Solventes/química , Espectrofotometría Atómica , Sustancias Reactivas al Ácido Tiobarbitúrico/metabolismoRESUMEN
Here we report the isolation and structure elucidation of a new phytotoxic neo-clerodane diterpenoid, royleanumin (1), along with known constituents, ursolic acid and beta-amyrin. Its structure was deduced by spectral studies including NMR spectroscopy, mass spectrometric techniques and by the comparison with reported data. The compound was assayed for its phytotoxicity against lettuce seedlings. Royleanumin (1) exhibit strong activity and can be proposed as lead chemical for the development of a new environmentally friendly herbicide.