RESUMEN
Anthocyanins are polyphenolic compounds present in grapes that are responsible for the initial colour of red wine and their incorporation into derived pigments leads to long term colour stability. The ability to predict the effect of process variables, either controlled by winemakers or that naturally change throughout fermentation, on the extraction of anthocyanins is vital to producing red wine of high quality. A 23 factorial experiment with additional points located at central factor conditions was used to determine the impact of temperature, sugar and ethanol concentrations on the mass transfer properties of anthocyanins from fresh Pinot noir grape solids. Factor conditions were chosen to replicate ethanol and sugar concentrations that would be found in a 14% red wine and its respective unfermented juice. A previously described mathematical model was applied to anthocyanin extraction curves to determine mass transfer coefficients and distribution constants for the generation of response surface models able to predict these mass transfer variables for dynamic fermentation scenarios. The coefficient of determination for the model solution exceeded 0.94 in all cases, demonstrating a good agreement between experimental and mathematically-derived anthocyanin concentrations. Following this, simulations of anthocyanin extraction under fermentation conditions were conducted using a previously developed wine fermentation model allowing for the prediction of extraction rates and anthocyanin concentrations under various winemaking scenarios. The extraction simulations predicted a previously observed but so far undescribed anthocyanin extraction pattern during fermentation.
Asunto(s)
Antocianinas/análisis , Fermentación , Modelos Teóricos , Vino/análisis , Color , Etanol/análisis , Manipulación de Alimentos , Viscosidad , Vitis/químicaRESUMEN
The colour of red wine is largely determined by the concentration of anthocyanins that are extracted from grape skins during fermentation. Because colour is a key parameter in determining the overall quality of the finished product, understanding the effect of processing variables on anthocyanin extraction is critical for producing a red wine with the desired sensorial characteristics. In this study, the effect of convective conditions (natural and forced) on the mass transfer properties of malvidin-3-glucoside (M3G) from pre-fermentative grape solids was explored at various liquid phase conditions representing stages of fermentation. A mathematical model that separates solid and liquid phase mass transfer parameters was applied to experimental extraction curves, and in all cases, provided a coefficient of determination exceeding 0.97. Calculated mass transfer coefficients indicated that under forced convective conditions, the extraction process was controlled by internal diffusion whereas under natural convection, both internal diffusion and liquid-phase mass transfer were relevant in determining the overall extraction rate. Predictive simulations of M3G extraction during active fermentation were accomplished by incorporating the current results with a previously developed fermentation model, providing insight into the effect of a dynamic liquid phase on anthocyanin extraction.
Asunto(s)
Antocianinas/química , Antocianinas/aislamiento & purificación , Convección , Fermentación , Vitis/química , Algoritmos , Fraccionamiento Químico , Simulación por Computador , Modelos Teóricos , Vino/análisisRESUMEN
Extraction of grape components is a key consideration for red winemaking. The impact of changing process variables on mass transfer properties of anthocyanins from fresh pre-fermentative red grape solids under forced convective conditions was explored using the dominant red grape anthocyanin, malvidin-3-glucoside (M3G) as a model solute. A two level full factorial design was implemented to investigate effects of temperature, sugar and ethanol on mass transfer properties. Factor levels were chosen to simulate conditions found at various points during the maceration and fermentation steps of the red winemaking process. A rigorous mathematical model was developed and applied to experimental extraction curves, allowing the separation of mass transport properties in liquid and solid phases in a wine-like system, for the first time. In all cases, the coefficient of determination exceeded 0.92, indicating good agreement between experimental and mathematically-solved M3G concentrations. For the conditions studied, internal mass transfer was found to limit M3G extraction and changes to the liquid phase composition and temperature influence the distribution constant. Surface response models of mass transfer parameters were developed to allow future simulations of fermentation scenarios aimed at maximising the extraction potential of M3G.