RESUMEN

In the current study we have investigated the protein content of blood plasma deoxyribonucleoprotein complexes. The complexes were isolated using affinity chromatography with immobilized polyclonal anti-histone antibodies. Proteins were separated by SDS PAAGE and identified by MALDI-TOF mass-spectrometry. 111 and 56 proteins (excluding histones), respectively, were identified with a good score in deoxyribonucleoprotein complexes of healthy females and breast cancer patients. However, only four of these proteins were found in 30 % of all samples. Fourteen proteins previously described as tumor specific proteins were found in cancer patients whereas not one of them was found in healthy individuals. The data obtained demonstrate the involvement of different cellular and extracellular proteins in circulating cell-free DNA.

Asunto(s)

Neoplasias de la Mama/metabolismo , Desoxirribonucleoproteínas/metabolismo , Proteínas de Neoplasias/metabolismo , Nucleoproteínas/metabolismo , Anticuerpos/inmunología , Anticuerpos Inmovilizados/inmunología , Anticuerpos Inmovilizados/metabolismo , Neoplasias de la Mama/sangre , Cromatografía de Afinidad/métodos , ADN/sangre , ADN/genética , ADN/metabolismo , Desoxirribonucleoproteínas/sangre , Electroforesis en Gel de Poliacrilamida , Femenino , Histonas/inmunología , Humanos , Proteínas de Neoplasias/sangre , Nucleoproteínas/sangre , Unión Proteica , Espectrometría de Masa por Láser de Matriz Asistida de Ionización Desorción