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1.
Sci Total Environ ; 753: 141817, 2021 Jan 20.
Artículo en Inglés | MEDLINE | ID: mdl-32891992

RESUMEN

Mangroves are effective blue carbon sinks and are the most carbon rich ecosystems on earth. However, their areal extent has declined by over one-third in recent decades. Degraded mangrove forests result in reduced carbon captured and lead to release of stored carbon into the atmosphere by CO2 emission. The aim of this study was to assess changes in carbon dynamics in a gradually degrading mangrove forest on Bonaire, Dutch Caribbean. Remote sensing techniques were applied to estimate the distribution of intact and degraded mangroves. Forest structure, sediment carbon storage, sediment CO2 effluxes and dissolved organic and inorganic carbon in pore and surface waters across intact and degraded parts were assessed. On average intact mangroves showed 31% sediment organic carbon in the upper 30 cm compared to 20% in degraded mangrove areas. A loss of 1.51 MgCO2 ha-1 yr-1 for degraded sites was calculated. Water samples showed a hypersaline environment in the degraded mangrove area averaging 93 which may have caused mangrove dieback. Sediment CO2 efflux within degraded sites was lower than values from other studies where degradation was caused by clearing or cutting, giving new insights into carbon dynamics in slowly degrading mangrove systems. Results of water samples agreed with previous studies where inorganic carbon outwelled from mangroves might enhance ecosystem connectivity by potentially buffering ocean acidification locally. Wetlands will be impacted by a variety of stressors resulting from a changing climate. Results from this study could inform scientists and stakeholders on how combined stresses, such as climate change with salinity intrusion may impact mangrove's blue carbon sink potential and highlight the need of future comparative studies of intact versus degraded mangrove stands.

2.
Med Klin Intensivmed Notfmed ; 112(7): 589-596, 2017 Oct.
Artículo en Alemán | MEDLINE | ID: mdl-28875277

RESUMEN

An average of 50-80% of patients treated in the intensive care unit is affected by disturbances of neuromuscular functions due to damage to the nerves and muscles, which has led to the terms critical illness polyneuropathy and myopathy. Both components occur in 30-50% of patients, while the others predominantly show a pure myopathy, while pure neuropathy is rare. Meanwhile, the descriptive term of the concept as intensive care unit-acquired weakness (ICUAW) is preferred. The most significant risk factors for the development of ICUAW are sepsis, multiorgan dysfunction and acute respiratory distress syndrome (ARDS). In at least one third of patients, persistent impairment by paralysis, sensory disturbances and balance problems persist when they leave the ICU. At approximately 10%, these leg-accentuated and highly everyday relevant disorders persist over the first year after ICU therapy. Pure myopathy rarely leads to residual disturbances, while the neuropathic component is responsible for long-term impairments.


Asunto(s)
Enfermedad Crítica , Enfermedades Musculares , Polineuropatías , Sepsis , Humanos , Unidades de Cuidados Intensivos , Enfermedades Musculares/terapia , Polineuropatías/terapia
3.
Vitam Horm ; 104: 367-404, 2017.
Artículo en Inglés | MEDLINE | ID: mdl-28215302

RESUMEN

The p75 neurotrophin receptor (p75NTR, a.k.a. CD271), a transmembrane glycoprotein and a member of the tumor necrosis family (TNF) of receptors, was originally identified as a nerve growth factor receptor in the mid-1980s. While p75NTR is recognized to have important roles during neural development, its presence in both neural and nonneural tissues clearly supports the potential to mediate a broad range of functions depending on cellular context. Using an unbiased in vivo selection paradigm for genes underlying the invasive behavior of glioma, a critical characteristic that contributes to poor clinical outcome for glioma patients, we identified p75NTR as a central regulator of glioma invasion. Herein we review the expanding role that p75NTR plays in glioma progression with an emphasis on how p75NTR may contribute to the treatment refractory nature of glioma. Based on the observation that p75NTR is expressed and functional in two critical glioma disease reservoirs, namely, the highly infiltrative cells that evade surgical resection, and the radiation- and chemotherapy-resistant brain tumor-initiating cells (also referred to as brain tumor stem cells), we propose that p75NTR and its myriad of downstream signaling effectors represent rationale therapeutic targets for this devastating disease. Lastly, we provide the provocative hypothesis that, in addition to the well-documented cell autonomous signaling functions, the neurotrophins, and their respective receptors, contribute in a cell nonautonomous manner to drive the complex cellular and molecular composition of the brain tumor microenvironment, an environment that fuels tumorigenesis.


Asunto(s)
Carcinogénesis/metabolismo , Glioma/metabolismo , Modelos Neurológicos , Factores de Crecimiento Nervioso/metabolismo , Proteínas del Tejido Nervioso/agonistas , Receptores de Factor de Crecimiento Nervioso/agonistas , Transducción de Señal , Animales , Antineoplásicos/uso terapéutico , Carcinogénesis/patología , Resistencia a Antineoplásicos , Glioma/tratamiento farmacológico , Glioma/inmunología , Glioma/patología , Humanos , Macrófagos/citología , Macrófagos/inmunología , Macrófagos/metabolismo , Macrófagos/patología , Microglía/citología , Microglía/inmunología , Microglía/metabolismo , Microglía/patología , Invasividad Neoplásica , Proteínas de Neoplasias/agonistas , Proteínas de Neoplasias/metabolismo , Células Madre Neoplásicas/inmunología , Células Madre Neoplásicas/metabolismo , Células Madre Neoplásicas/patología , Proteínas del Tejido Nervioso/metabolismo , Células-Madre Neurales/citología , Células-Madre Neurales/inmunología , Células-Madre Neurales/metabolismo , Células-Madre Neurales/patología , Receptores de Factor de Crecimiento Nervioso/metabolismo , Transducción de Señal/efectos de los fármacos , Microambiente Tumoral/efectos de los fármacos
4.
Oncogene ; 35(11): 1411-22, 2016 Mar 17.
Artículo en Inglés | MEDLINE | ID: mdl-26119933

RESUMEN

The invasive nature of glioblastoma renders them incurable by current therapeutic interventions. Using a novel invasive human glioma model, we previously identified the neurotrophin receptor p75(NTR) (aka CD271) as a mediator of glioma invasion. Herein, we provide evidence that preventing phosphorylation of p75(NTR) on S303 by pharmacological inhibition of PKA, or by a mutational strategy (S303G), cripples p75(NTR)-mediated glioma invasion resulting in serine phosphorylation within the C-terminal PDZ-binding motif (SPV) of p75(NTR). Consistent with this, deletion (ΔSPV) or mutation (SPM) of the PDZ motif results in abrogation of p75(NTR)-mediated invasion. Using a peptide-based strategy, we identified PDLIM1 as a novel signaling adaptor for p75(NTR) and provide the first evidence for a regulated interaction via S425 phosphorylation. Importantly, PDLIM1 was shown to interact with p75(NTR) in highly invasive patient-derived glioma stem cells/tumor-initiating cells and shRNA knockdown of PDLIM1 in vitro and in vivo results in complete ablation of p75(NTR)-mediated invasion. Collectively, these data demonstrate a requirement for a regulated interaction of p75(NTR) with PDLIM1 and suggest that targeting either the PDZ domain interactions and/or the phosphorylation of p75(NTR) by PKA could provide therapeutic strategies for patients with glioblastoma.


Asunto(s)
Neoplasias Encefálicas/patología , Glioblastoma/patología , Proteínas con Dominio LIM/genética , Proteínas del Tejido Nervioso/genética , Receptores de Factor de Crecimiento Nervioso/genética , Factores de Transcripción/genética , Animales , Línea Celular Tumoral , Proteínas Quinasas Dependientes de AMP Cíclico/antagonistas & inhibidores , Femenino , Humanos , Ratones , Ratones SCID , Invasividad Neoplásica/patología , Proteínas del Tejido Nervioso/metabolismo , Dominios PDZ/genética , Fosforilación , Interferencia de ARN , ARN Interferente Pequeño/genética , Receptores de Factor de Crecimiento Nervioso/metabolismo , Transducción de Señal/fisiología
5.
Gene Ther ; 11(21): 1579-89, 2004 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-15372068

RESUMEN

Brain and leptomeningeal metastases are common in breast cancer patients and our current treatments are ineffective. Reovirus type 3 is a replication competent, naturally occurring virus that usurps the activated Ras-signaling pathway (or an element thereof) of tumor cells and lyses them but leaves normal cells relatively unaffected. In this study we evaluated reovirus as an experimental therapeutic in models of central nervous system (CNS) metastasis from breast cancer. We found all breast cancer cell lines tested were susceptible to reovirus, with > 50% of these cells lysed within 72 h of infection. In vivo neurotoxicity studies showed only mild local inflammation at the injection site and mild communicating hydrocephalus with neither diffuse encephalitis nor behavioral abnormalities at the therapeutically effective dose of reovirus (intracranial) (ie 10(7) plaque-forming units) or one dose level higher. In vivo, a single intratumoral administration of reovirus significantly reduced the size of tumors established from two human breast cancer cell lines and significantly prolonged survival. Intrathecal administration of reovirus also remarkably prolonged survival in an immunocompetent racine model of leptomeningeal metastases. These data suggest that the evaluation of reovirus as an experimental therapeutic for CNS metastases from breast cancer is warranted.


Asunto(s)
Neoplasias Encefálicas/secundario , Neoplasias Encefálicas/terapia , Neoplasias de la Mama/terapia , Orthoreovirus Mamífero 3 , Infecciones por Reoviridae/complicaciones , Animales , Neoplasias Encefálicas/virología , Muerte Celular , Línea Celular Tumoral , Femenino , Proteínas Fluorescentes Verdes/genética , Humanos , Inyecciones Intralesiones , Dosificación Letal Mediana , Neoplasias Meníngeas/secundario , Neoplasias Meníngeas/terapia , Neoplasias Meníngeas/virología , Ratones , Ratones Desnudos , Modelos Animales , Neoplasias Experimentales , Transfección/métodos
6.
J Natl Cancer Inst ; 93(12): 903-12, 2001 Jun 20.
Artículo en Inglés | MEDLINE | ID: mdl-11416111

RESUMEN

BACKGROUND: Reovirus is a naturally occurring oncolytic virus that usurps activated Ras-signaling pathways of tumor cells for its replication. Ras pathways are activated in most malignant gliomas via upstream signaling by receptor tyrosine kinases. The purpose of this study was to determine the effectiveness of reovirus as an experimental treatment for malignant gliomas. METHODS: We investigated whether reovirus would infect and lyse human glioma cell lines in vitro. We also tested the effect of injecting live reovirus in vivo on human gliomas grown subcutaneously or orthotopically (i.e., intracerebrally) in mice. Finally, reovirus was tested ex vivo against low-passage cell lines derived from human glioma specimens. All P values were two-sided. RESULTS: Reovirus killed 20 (83%) of 24 established malignant glioma cell lines tested. It caused a dramatic and often complete tumor regression in vivo in two subcutaneous (P =.0002 for both U251N and U87) and in two intracerebral (P =.0004 for U251N and P =.0009 for U87) human malignant glioma mouse models. As expected, serious toxic effects were found in these severely immunocompromised hosts. In a less immunocompromised mouse model, a single intratumoral inoculation of live reovirus led to a dramatic prolongation of survival (compared with control mice treated with dead virus; log-rank test, P<.0001 for both U251N and U87 cell lines). The animals treated with live virus also appeared to be healthier and gained body weight (P =.0001). We then tested the ability of reovirus to infect and kill primary cultures of brain tumors removed from patients and found that it killed nine (100%) of nine glioma specimens but none of the cultured meningiomas. CONCLUSIONS: Reovirus has potent activity against human malignant gliomas in vitro, in vivo, and ex vivo. Oncolysis with reovirus may be a potentially useful treatment for a broad range of human cancers.


Asunto(s)
Neoplasias Encefálicas/terapia , Glioma/terapia , Orthoreovirus Mamífero 3/fisiología , Animales , Neoplasias Encefálicas/patología , Neoplasias Encefálicas/virología , Femenino , Glioma/patología , Glioma/virología , Humanos , Masculino , Orthoreovirus Mamífero 3/aislamiento & purificación , Ratones , Ratones Endogámicos NOD , Ratones Desnudos , Ratones SCID , Tasa de Supervivencia , Trasplante Heterólogo , Células Tumorales Cultivadas
7.
Proc Natl Acad Sci U S A ; 98(10): 5804-8, 2001 May 08.
Artículo en Inglés | MEDLINE | ID: mdl-11331770

RESUMEN

Retinopathy of prematurity is a blinding disease, initiated by lack of retinal vascular growth after premature birth. We show that lack of insulin-like growth factor I (IGF-I) in knockout mice prevents normal retinal vascular growth, despite the presence of vascular endothelial growth factor, important to vessel development. In vitro, low levels of IGF-I prevent vascular endothelial growth factor-induced activation of protein kinase B (Akt), a kinase critical for endothelial cell survival. Our results from studies in premature infants suggest that if the IGF-I level is sufficient after birth, normal vessel development occurs and retinopathy of prematurity does not develop. When IGF-I is persistently low, vessels cease to grow, maturing avascular retina becomes hypoxic and vascular endothelial growth factor accumulates in the vitreous. As IGF-I increases to a critical level, retinal neovascularization is triggered. These data indicate that serum IGF-I levels in premature infants can predict which infants will develop retinopathy of prematurity and further suggests that early restoration of IGF-I in premature infants to normal levels could prevent this disease.


Asunto(s)
Supervivencia Celular , Factores de Crecimiento Endotelial/metabolismo , Endotelio Vascular/metabolismo , Factor I del Crecimiento Similar a la Insulina/metabolismo , Linfocinas/metabolismo , Vasos Retinianos/metabolismo , Retinopatía de la Prematuridad/metabolismo , Transducción de Señal , Secuencia de Bases , Cartilla de ADN , Endotelio Vascular/citología , Humanos , Recién Nacido , Vasos Retinianos/citología , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular
8.
Urology ; 57(4 Suppl 1): 143-7, 2001 Apr.
Artículo en Inglés | MEDLINE | ID: mdl-11295614

RESUMEN

Prostate cancer is a multistep process in which progression rather than initiation may be the rate-limiting step. A strong possibility is that prostatic intraepithelial neoplasia lesions that switch to angiogenic phenotype eventually progress to cancer. However, it is a challenging task to quantitate angiogenesis in preneoplastic lesions. A promising approach to measuring angiogenesis involves real-time TaqMan polymerase chain reaction to quantitate mRNAs encoding a panel of angiogenesis markers. This highly sensitive molecular technique has potential for quantitating angiogenesis in clinical settings and can be used as a high-throughput screening procedure in prostate cancer clinical trials.


Asunto(s)
Neovascularización Patológica/diagnóstico , Reacción en Cadena de la Polimerasa/métodos , Neoplasia Intraepitelial Prostática/irrigación sanguínea , Neoplasias de la Próstata/irrigación sanguínea , Animales , Biomarcadores/análisis , Progresión de la Enfermedad , Factores de Crecimiento Endotelial/metabolismo , Silenciador del Gen , Genes Supresores de Tumor , Humanos , Linfocinas/metabolismo , Masculino , Melanoma/metabolismo , Ratones , Neovascularización Patológica/metabolismo , Fenotipo , Neoplasia Intraepitelial Prostática/genética , Neoplasia Intraepitelial Prostática/metabolismo , Neoplasias de la Próstata/genética , Neoplasias de la Próstata/metabolismo , Trasplante Heterólogo , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular
10.
Nat Med ; 5(12): 1390-5, 1999 Dec.
Artículo en Inglés | MEDLINE | ID: mdl-10581081

RESUMEN

Although insulin-like growth factor 1 (IGF-1) has been associated with retinopathy, proof of a direct relationship has been lacking. Here we show that an IGF-1 receptor antagonist suppresses retinal neovascularization in vivo, and infer that interactions between IGF-1 and the IGF-1 receptor are necessary for induction of maximal neovascularization by vascular endothelial growth factor (VEGF). IGF-1 receptor regulation of VEGF action is mediated at least in part through control of VEGF activation of p44/42 mitogen-activated protein kinase, establishing a hierarchical relationship between IGF-1 and VEGF receptors. These findings establish an essential role for IGF-1 in angiogenesis and demonstrate a new target for control of retinopathy. They also explain why diabetic retinopathy initially increases with the onset of insulin treatment. IGF-1 levels, low in untreated diabetes, rise with insulin therapy, permitting VEGF-induced retinopathy.


Asunto(s)
Factores de Crecimiento Endotelial/fisiología , Linfocinas/fisiología , Neovascularización Patológica/fisiopatología , Receptor IGF Tipo 1/fisiología , Vasos Retinianos/fisiología , Animales , Inhibidores de Crecimiento/farmacología , Humanos , Insulina/farmacología , Factor I del Crecimiento Similar a la Insulina/análogos & derivados , Factor I del Crecimiento Similar a la Insulina/farmacología , Isquemia/etiología , Isquemia/fisiopatología , Isquemia/prevención & control , Ratones , Ratones Endogámicos C57BL , Neovascularización Patológica/etiología , Neovascularización Patológica/prevención & control , Proteínas Tirosina Quinasas/antagonistas & inhibidores , Proteínas Tirosina Quinasas Receptoras/fisiología , Receptor IGF Tipo 1/antagonistas & inhibidores , Receptores de Factores de Crecimiento/fisiología , Receptores de Factores de Crecimiento Endotelial Vascular , Vasos Retinianos/efectos de los fármacos , Transducción de Señal , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular
11.
J Cell Biol ; 138(2): 411-21, 1997 Jul 28.
Artículo en Inglés | MEDLINE | ID: mdl-9230082

RESUMEN

According to the current theory of retrograde signaling, NGF binds to receptors on the axon terminals and is internalized by receptor-mediated endocytosis. Vesicles with NGF in their lumina, activating receptors in their membranes, travel to the cell bodies and initiate signaling cascades that reach the nucleus. This theory predicts that the retrograde appearance of activated signaling molecules in the cell bodies should coincide with the retrograde appearance of the NGF that initiated the signals. However, we observed that NGF applied locally to distal axons of rat sympathetic neurons in compartmented cultures produced increased tyrosine phosphorylation of trkA in cell bodies/ proximal axons within 1 min. Other proximal proteins, including several apparently localized in cell bodies, displayed increased tyrosine phosphorylation within 5-15 min. However, no detectable 125I-NGF appeared in the cell bodies/proximal axons within 30-60 min of its addition to distal axons. Even if a small, undetectable fraction of transported 125I-NGF was internalized and loaded onto the retrograde transport system immediately after NGF application, at least 3-6 min would be required for the NGF that binds to receptors on distal axons just outside the barrier to be transported to the proximal axons just inside the barrier. Moreover, it is unlikely that the tiny fraction of distal axon trk receptors located near the barrier alone could produce a measurable retrograde trk phosphorylation even if enough time was allowed for internalization and transport of these receptors. Thus, our results provide strong evidence that NGF-induced retrograde signals precede the arrival of endocytotic vesicles containing the NGF that induced them. We further suggest that at least some components of the retrograde signal are carried by a propagation mechanism.


Asunto(s)
Factores de Crecimiento Nervioso/metabolismo , Neuronas/metabolismo , Proteínas Proto-Oncogénicas/metabolismo , Proteínas Tirosina Quinasas Receptoras/metabolismo , Receptores de Factor de Crecimiento Nervioso/metabolismo , Tirosina/metabolismo , Animales , Transporte Biológico , Compartimento Celular , Células Cultivadas , Factores de Crecimiento Nervioso/farmacología , Neuritas , Fosforilación , Ratas , Ratas Sprague-Dawley , Receptor trkA , Transducción de Señal/fisiología , Ganglio Cervical Superior/citología
12.
Dev Biol ; 184(1): 1-9, 1997 Apr 01.
Artículo en Inglés | MEDLINE | ID: mdl-9142978

RESUMEN

To examine the cellular mechanisms whereby distally derived growth factors regulate nuclear responses in neurons, we have utilized compartmented cultures of sympathetic neurons to examine the regulation of two nerve growth factor (NGF)-inducible genes, tyrosine hydroxylase (TH) and p75 neurotrophin receptor (p75NTR). These studies demonstrate that NGF can signal retrogradely to mediate the induction of TH and p75NTR mRNAs. However, quantitative differences occurred as a function of the spatial localization of NGF exposure; application of NGF to cell bodies and proximal axons elicited peak levels of neuronal gene expression that were two- to threefold higher than when NGF was applied to distal axons alone. Furthermore, neurons responding maximally to NGF on distal axons were still able to respond to NGF administered to cell bodies and proximal axons. Biochemical analysis indicated that this difference in responsiveness was not due to differences in the number of TrkA/NGF receptors in the two compartments. Thus, although NGF signals retrogradely to mediate nuclear responses, the magnitude of these responses differs as a function of the spatial location of the activated NGF receptor:ligand complex. Moreover, these data suggest that neurons may be able to respond to a second cellular source of neurotrophins, even when target-derived neurotrophins are not limiting.


Asunto(s)
Regulación de la Expresión Génica/fisiología , Factores de Crecimiento Nervioso/farmacología , Neuronas/fisiología , Animales , Animales Recién Nacidos , Compartimento Celular , Células Cultivadas , Regulación Enzimológica de la Expresión Génica/fisiología , Neuritas , Neuronas/química , Proteínas Proto-Oncogénicas/análisis , Proteínas Proto-Oncogénicas/genética , ARN Mensajero/biosíntesis , Ratas , Ratas Sprague-Dawley , Proteínas Tirosina Quinasas Receptoras/análisis , Proteínas Tirosina Quinasas Receptoras/genética , Receptor de Factor de Crecimiento Nervioso , Receptor trkA , Receptores de Factor de Crecimiento Nervioso/análisis , Receptores de Factor de Crecimiento Nervioso/genética , Sistema Nervioso Simpático/citología , Tirosina 3-Monooxigenasa/biosíntesis , Tirosina 3-Monooxigenasa/genética
13.
Proc Natl Acad Sci U S A ; 94(25): 13612-7, 1997 Dec 09.
Artículo en Inglés | MEDLINE | ID: mdl-9391074

RESUMEN

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor, is a cytokine of central importance for the angiogenesis associated with cancers and other pathologies. Because angiogenesis often involves endothelial cell (EC) migration and proliferation within a collagen-rich extracellular matrix, we investigated the possibility that VEGF promotes neovascularization through regulation of collagen receptor expression. VEGF induced a 5- to 7-fold increase in dermal microvascular EC surface protein expression of two collagen receptors-the alpha1beta1 and alpha2beta1 integrins-through induction of mRNAs encoding the alpha1 and alpha2 subunits. In contrast, VEGF did not induce increased expression of the alpha3beta1 integrin, which also has been implicated in collagen binding. Integrin alpha1-blocking and alpha2-blocking antibodies (Ab) each partially inhibited attachment of microvascular EC to collagen I, and alpha1-blocking Ab also inhibited attachment to collagen IV and laminin-1. Induction of alpha1beta1 and alpha2beta1 expression by VEGF promoted cell spreading on collagen I gels which was abolished by a combination of alpha1-blocking and alpha2-blocking Abs. In vivo, a combination of alpha1-blocking and alpha2-blocking Abs markedly inhibited VEGF-driven angiogenesis; average cross-sectional area of individual new blood vessels was reduced 90% and average total new vascular area was reduced 82% without detectable effects on the pre-existing vasculature. These data indicate that induction of alpha1beta1 and alpha2beta1 expression by EC is an important mechanism by which VEGF promotes angiogenesis and that alpha1beta1 and alpha2beta1 antagonists may prove effective in inhibiting VEGF-driven angiogenesis in cancers and other important pathologies.


Asunto(s)
Factores de Crecimiento Endotelial/farmacología , Integrinas/fisiología , Linfocinas/farmacología , Neovascularización Fisiológica/efectos de los fármacos , Animales , Anticuerpos/farmacología , Adhesión Celular , Células Cultivadas , Colágeno/metabolismo , Factores de Crecimiento Endotelial/fisiología , Endotelio Vascular/citología , Endotelio Vascular/metabolismo , Femenino , Humanos , Técnicas In Vitro , Integrina alfa1beta1 , Integrinas/antagonistas & inhibidores , Integrinas/genética , Linfocinas/fisiología , Ratones , Ratones Desnudos , ARN Mensajero/genética , Receptores de Colágeno , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular
14.
Biochim Biophys Acta ; 1314(1-2): 13-24, 1996 Nov 08.
Artículo en Inglés | MEDLINE | ID: mdl-8972713

RESUMEN

Osteopontin (OPN) is a secreted adhesive glycoprotein with a gly-arg-gly-asp-ser (GRGDS) cell binding domain. Several independent studies have suggested that OPN functions in tumor growth and metastasis, and one likely possibility is that OPN facilitates tumor invasion by promoting tumor cell migration. Consistent with this hypothesis, immobilized OPN promoted concentration-dependent tumor cell migration (i.e., haptotaxis) in modified Boyden chambers. In particular, cleavage of OPN by thrombin, which likely occurs in the tumor microenvironment, resulted in enhancement of OPNs haptotactic activity; and assays performed with purified preparations of the two individual OPN thrombin-cleavage fragments demonstrated that all detectable activity was associated with the GRGDS-containing fragment. In contrast to the activity of both OPN and its GRGDS-containing fragment in promoting haptotaxis, neither of these proteins in solution promoted chemotaxis, indicating that each must be immobilized to promote cell migration. In haptotaxis assays, antibody LM609 to integrin alpha v beta 3 blocked > 80% cell migration towards the GRGDS-containing OPN fragment, implicating alpha v beta 3 as its principal functional receptor. In comparison with equimolar quantities of other adhesive proteins, the GRGDS-containing OPN thrombin-cleavage fragment was not only > 2-fold more effective than intact OPN at promoting haptotaxis, but also > 8-fold and > 6-fold more effective than fibrinogen and vitronectin, respectively, indicating that this OPN fragment is highly active relative to other alpha v beta 3 ligands.


Asunto(s)
Movimiento Celular/efectos de los fármacos , Oligopéptidos/química , Fragmentos de Péptidos/farmacología , Sialoglicoproteínas/química , Trombina/química , Secuencia de Aminoácidos , Humanos , Hidrólisis , Datos de Secuencia Molecular , Osteopontina , Fragmentos de Péptidos/metabolismo , Receptores de Vitronectina/metabolismo , Células Tumorales Cultivadas
15.
J Cell Biol ; 135(3): 701-9, 1996 Nov.
Artículo en Inglés | MEDLINE | ID: mdl-8909544

RESUMEN

Growing axons receive a substantial supply of tubulin and other proteins delivered from sites of synthesis in the cell body by slow axonal transport. To investigate the mechanism of tubulin transport most previous studies have used in vitro models in which the transport of microtubules can be visualized during brief periods of growth. To investigate total tubulin transport in neurons displaying substantial growth over longer periods, we used rat sympathetic neurons in compartmented cultures. Tubulin synthesized during pulses of [35S]methionine was separated from other proteins by immunoprecipitation with monoclonal antibodies to alpha and beta tubulin, further separated on SDS-PAGE, and quantified by phosphorimaging. Results showed that 90% of newly synthesized tubulin moved into the distal axons within 2 d. Furthermore, the leading edge of tubulin was transported at a velocity faster than 4 mm/d, more than four times the rate of axon elongation. This velocity did not diminish with distance from the cell body, suggesting that the transport system is capable of distributing newly synthesized tubulin to growth cones throughout the axonal tree. Neither diffusion nor the an mass transport of axonal microtubules can account for the velocity and magnitude of tubulin transport that was observed. Thus, it is likely that most of the newly synthesized tubulin was supplied to the growing axonal tree in subunit form such as a heterodimer or an oligomer considerably smaller than a microtubule.


Asunto(s)
Transporte Axonal/fisiología , Axones/metabolismo , Ganglio Cervical Superior/citología , Tubulina (Proteína)/metabolismo , Animales , Animales Recién Nacidos , Transporte Biológico , Técnicas de Cultivo de Célula , Células Cultivadas , Microtúbulos/metabolismo , Neuronas/citología , Ratas , Ratas Sprague-Dawley , Tubulina (Proteína)/biosíntesis
17.
Am J Pathol ; 149(1): 293-305, 1996 Jul.
Artículo en Inglés | MEDLINE | ID: mdl-8686754

RESUMEN

We have identified several mechanisms by which the angiogenic cytokine vascular permeability factor/vascular endothelial growth factor (VPF/VEGF) likely regulates endothelial cells (EC) migration. VPF/VEGF induced dermal microvascular EC expression of mRNAs encoding the alphav and beta3 integrin subunits resulting in increased levels of the alphavbeta3 heterodimer at the cell surface, and VPF/VEGF also induced mRNA encoding osteopontin (OPN), an alphavbeta3 ligand. OPN promoted EC migration in vitro; and VPF/VEGF induction of alphavbeta3 was accompanied by increased EC migration toward OPN. Because thrombin cleavage of OPN results in substantial enhancement of OPN's adhesive properties, and because VPF/VEGF promotes increased microvascular permeability leading to activation of the extrinsic coagulation pathway, we also investigated whether VPF/VEGF facilitates thrombin cleavage of OPN in vivo. Consistent with this hypothesis, co-injection of VPF/VEGF together with OPN resulted in rapid cleavage of OPN by endogenous thrombin. Furthermore, in comparison with native OPN, thrombin-cleaved OPN stimulated a greater rate of EC migration in vitro, which was additive to the increased migration associated with induction of alpha v beta 3. Thus, these data demonstrate cooperative mechanisms for VPF/VEGF regulation of EC migration involving the alphavbeta3 integrin, the alphavbeta3 ligand OPN, and thrombin cleavage of OPN. These findings also illustrate an operational link between VPF/VEGF induction of EC gene expression and VPF/VEGF enhancement of microvascular permeability, suggesting that these distinct biological activities may act accordingly to stimulate EC migration during angiogenesis.


Asunto(s)
Movimiento Celular/fisiología , Factores de Crecimiento Endotelial/fisiología , Endotelio Vascular/fisiología , Integrinas/fisiología , Linfocinas/fisiología , Receptores de Vitronectina/fisiología , Sialoglicoproteínas/fisiología , Animales , Adhesión Celular/efectos de los fármacos , Adhesión Celular/fisiología , Movimiento Celular/efectos de los fármacos , Células Cultivadas , Factores de Crecimiento Endotelial/farmacología , Endotelio Vascular/efectos de los fármacos , Femenino , Cobayas , Humanos , Recién Nacido , Linfocinas/farmacología , Masculino , Osteopontina , ARN Mensajero/análisis , ARN Mensajero/efectos de los fármacos , Sialoglicoproteínas/farmacología , Trombina/fisiología , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular
18.
J Perinat Med ; 24(6): 703-6, 1996.
Artículo en Inglés | MEDLINE | ID: mdl-9120756

RESUMEN

Thrombocytopenia can be a pathophysiological feature of pregnancy. In the case reported, the thrombocyte count was reduced to 1% of normal (1 x 10(9) thrombocytes/l) at 28 weeks of gestation. In chronological order, the patient showed epistaxis, macrohematuria and gingival, conjunctival, intracerebral and pulmonary bleeding. The latter was life-threatening. An emergency splenectomy was undertaken, without complications. The operation was followed by a massive increase in the thrombocyte count, reaching 200 x 10(9)/l four days later. Unfortunately, a premature rupture of the membranes, with signs of amnion infection, occurred on the seventh day. A Cesarean section was undertaken (30 weeks of gestation), without complications. Both mother and baby are in good health 10 months later. The newborn had a normal thrombocyte count at delivery and thereafter. The life-threatening hemorrhage of the mother, the delivery of an unaffected baby and the relatively quick remission after splenectomy suggest an upregulated destruction of thrombocytes by the maternal spleen. The increased level of Macrophage-Colony Stimulating Factor (M-CSF), a normal feature of pregnancy, has the potential to augment thrombocyte destruction by activating macrophages. The production of anti-thrombocyte antibodies, especially if localized in the spleen, could result in increased thrombocyte sequestration by macrophages with severe effects focused on the mother.


Asunto(s)
Complicaciones Hematológicas del Embarazo/cirugía , Esplenectomía , Trombocitopenia/cirugía , Adulto , Autoanticuerpos/sangre , Enfermedades Autoinmunes , Femenino , Hemorragia/etiología , Humanos , Enfermedades Pulmonares/etiología , Embarazo , Trombocitopenia/inmunología
19.
Clin Immunol Immunopathol ; 77(1): 69-74, 1995 Oct.
Artículo en Inglés | MEDLINE | ID: mdl-7554486

RESUMEN

HIV infection is associated with polyclonal increase in serum immunoglobulins and with elevated titers of serum antibodies to a variety of self antigens, including anti-phospholipid antibodies. In the present study, we found a high prevalence of 46.8% of serum IgG anticardiolipin antibodies (ACA) in a group of 111 unselected HIV-seropositive individuals. The presence of ACA was correlated with that of IgG antibodies to endothelial cells (AECA) but not with that of anti-beta 2 glycoprotein I antibodies, that were only found in 7.4% of the patients. The presence of IgG ACA was not associated with detectable lupus anticoagulant activity, nor with a history of thrombosis. Serum titers of ACA were not correlated with absolute numbers of circulating CD4+ cells. We found no relationship between the presence and titers of ACA, hypergammaglobulinemia, and serum titers of natural IgG autoantibodies to a panel of self antigens. Our results suggest that increased titers of ACA in HIV infection result from a biased expansion of B cell clones producing natural autoantibodies.


Asunto(s)
Autoanticuerpos/inmunología , Cardiolipinas/inmunología , Endotelio Vascular/inmunología , Glicoproteínas/inmunología , Infecciones por VIH/inmunología , Adulto , Anciano , Femenino , Humanos , Inmunoglobulina G/inmunología , Inhibidor de Coagulación del Lupus/sangre , Masculino , Persona de Mediana Edad , beta 2 Glicoproteína I
20.
Int Arch Allergy Immunol ; 107(1-3): 233-5, 1995.
Artículo en Inglés | MEDLINE | ID: mdl-7542074

RESUMEN

Vascular permeability factor (VPF), also known as vascular endothelial growth factor (VEGF), is a multifunctional cytokine that is overexpressed in many transplantable animal and autochtonous human cancers, in healing wounds, and in chronic inflammatory disorders such as psoriasis and rheumatoid arthritis. All of these entities are characterized by angiogenesis, altered extracellular matrix, and variable degrees of hypoxia. In addition, two VPF/VEGF receptors, flt-1 and kdr, are overexpressed by endothelial cells that line the microvessels that supply these tumors/inflammatory reactions. On the basis of these and other data, we have proposed a model of angiogenesis in which VPF/VEGF plays a central role: this model is applicable to tumors and also to the angiogenesis that occurs in non-neoplastic processes.


Asunto(s)
Factores de Crecimiento Endotelial/farmacología , Inflamación/fisiopatología , Linfocinas/farmacología , Modelos Biológicos , Neoplasias/fisiopatología , Neovascularización Patológica/fisiopatología , Animales , Matriz Extracelular/metabolismo , Humanos , Proteínas de Neoplasias/fisiología , Proteínas Proto-Oncogénicas/fisiología , Proteínas Tirosina Quinasas Receptoras/fisiología , Receptores de Factores de Crecimiento/fisiología , Receptores de Factores de Crecimiento Endotelial Vascular , Factor A de Crecimiento Endotelial Vascular , Receptor 1 de Factores de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial Vascular , Cicatrización de Heridas/fisiología
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