RESUMEN

Alpha interferon (IFN-alpha) and acyclovir (ACV) are synergistic in their anti-herpes simplex virus activities. IFN-alpha treatment reduced the herpes simplex virus thymidine kinase (TK) activity present in cells 6 h postinfection, while steady-state levels of TK mRNA remained at or above the amount in infected, untreated cells. The inhibition of TK production by IFN-alpha treatment appeared to be transient and translational, not transcriptional.

Asunto(s)

Aciclovir/farmacología , Herpesvirus Humano 1/enzimología , Interferón-alfa/farmacología , Timidina Quinasa/metabolismo , Animales , Western Blotting , Línea Celular , Sinergismo Farmacológico , Humanos , Interferón alfa-2 , Mediciones Luminiscentes , Biosíntesis de Proteínas/efectos de los fármacos , ARN Viral/biosíntesis , Proteínas Recombinantes , Timidina Quinasa/antagonistas & inhibidores , Timidina Quinasa/biosíntesis , Transcripción Genética/efectos de los fármacos , Células Vero , Proteínas del Envoltorio Viral/biosíntesis