RESUMEN
A literatura atual discute múltiplas modalidades de imagem para acompanhar o processo de cicatrização da origem do ligamento suspensor do boleto (LSB) em equinos, mas nenhuma pode garantir que eles possuam fibras colágenas com calibre suficiente para suportar o retorno ao exercício. Já as técnicas morfológicas e bioquímicas, bem como a análise de birrefringência, podem ser mais apropriadas para caracterizar o processo de cicatrização e avaliar a eficiência do tratamento. O objetivo deste artigo é descrever procedimento simples que possibilita a coleta de amostras teciduais de boa qualidade e em sentido longitudinal, por biópsia em equinos em estação. Após antissepsia local, sedação e bloqueio do nervo palmar lateral no aspecto medial do osso acessório do carpo (OAC), o membro foi colocado em suspensão com o carpo flexionado em 90º; a agulha de biópsia guiada por ultrassom foi introduzida em sentido distoproximal, 11 a 13cm distal ao OAC, ângulo de 20º em relação ao LSB, até a região de sua origem. O equipamento foi disparado e coletou-se a amostra tecidual. Essa técnica possibilitou a coleta de fragmentos de boa qualidade para análise histológica e de birrefringência, sem reações adversas, podendo ser usada em modelos experimentais ou na prática clínica.(AU)
Asunto(s)
Animales , Ligamentos Redondos/diagnóstico por imagen , Caballos , Biopsia Guiada por Imagen/veterinariaRESUMEN
OBJECTIVE: To assess cases of suicidal behaviour in Guyana to characterize the suicidal profile of Guyanese at present. DESIGN AND METHODS: A nationally represented sample of 899 cases involved in suicidal behaviour, 555 who committed suicide and another 344 attempted suicides from 2010-2012, were surveyed using an epidemiologic study. Risk factors and other associations were identified by professionals through the use of a statistical review and the administration of questionnaires; the information was processed. RESULTS: Suicide was one of the biggest problems in Guyana as evidenced by the country being listed as having the highest estimated rate of suicide worldwide (44.3/100,000) with an mean of 200 lives being lost each year due to suicide. Data on respondents (patient or parents) were used for analysis. The most affected age group is 20 to 49 years (50%) Males committed suicide more frequently, with a ratio of almost 4:1 and the most commonly used methods in suicide cases were poisoning (pesticide / herbicide) with more than 65 % of cases, followed by hanging (>20%). East Indians accounted for >80 %; most of the cases were geographically concentrated in Regions 6,5,4,3 and 2 similar to the estimated cases of suicidal attempts. CONCLUSIONS: The profile of suicide had been well identified in Guyana, and it permits the development of a comprehensive multisectoral suicide prevention strategy to reduce the magnitude of suicidal behavior in Guyana.
Asunto(s)
Suicidio , Suicidio/estadística & datos numéricos , Intento de Suicidio , Intento de Suicidio/estadística & datos numéricos , Estudios Retrospectivos , GuyanaRESUMEN
Cytomegalovirus infection is the first cause of viral congenital infections. We studied the incidence of primary cytomegalovirus infection, searching for the presence of antibodies with an ELISA technique, in 939 pregnant women of a low socioeconomic level, attending a public outpatient clinic and 123 pregnant university students, attending a special outpatient clinic for students. The initially seronegative women were tested again during the second and third trimester of pregnancy to identify primary infections. The presence of cytomegalovirus congenital infection in the newborn of infected mothers was investigated isolating the virus in cell cultures from urine samples. There was a higher prevalence of infection among low socioeconomic status women (95 vs 69.9 per cent). Two women (one student and one coming from a low socioeconomic status) had a primary infection and in the newborn of the student, a congenital cytomegalovirus infection was detected. It is concluded that women's socioeconomic condition is not risk factor for cytomegalovirus primary infection during pregnancy
Asunto(s)
Humanos , Femenino , Embarazo , Recién Nacido , Adulto , Infecciones por Citomegalovirus/epidemiología , Citomegalovirus/patogenicidad , Ensayo de Inmunoadsorción Enzimática , Factores de Edad , Infecciones por Citomegalovirus/congénito , Infecciones por Citomegalovirus/transmisión , Anticuerpos/aislamiento & purificación , Factores Socioeconómicos , Técnica del Anticuerpo FluorescenteRESUMEN
Se realizó un estudio comparativo de prevalencia de herpes genital en dos grupos de embarazadas de distinto nivel socioeconómico, a través de la realización de una encuesta epidemiológica, estudio serológico y aislamiento viral. En la población de bajo nivel socioeconómico se detectó antecedentes de herpes genital en el 1,5%de ella. El 96%tenía anticuerpos anti-VHS, y en un 2%se aisló virus herpes simplex en el momento del parto sin detectarse ningún caso de herpes neonatal. En las embarazadas de alto nivel socioeconómico se obtuvo el antecedente de herpes genital en el 4,8%de ellas. Un 87%tenía anticuerpos antiherper y se aisló este virus en el 0,5%de esta población, detectándose un caso de herpes neonatal. Se discuten los factores que generan las diferencias entre las dos poblaciones
Asunto(s)
Herpes Genital/epidemiología , Pruebas Serológicas , Factores de Edad , Herpesviridae/aislamiento & purificación , Paridad , Factores SocioeconómicosRESUMEN
A survey of Candida albicans indicated that the organism was present at all sites sampled in a rain forest stream and in near-shore coastal waters of Puerto Rico. In the rain forest watershed no relationship existed between densities of fecal coliforms and densities of C. albicans. At two pristine sites in the rain forest watershed both C. albicans and Escherichia coli survived in diffusion chambers for extended periods of time. In near-shore coastal waters C. albicans and E. coli survival times in diffusion chambers were enhanced by effluent from a rum distillery. The rum distillery effluent had a greater effect on E. coli than on C. albicans survival in the diffusion chambers. These studies show that neither E. coli nor C. albicans organisms are good indicators of recent fecal contamination in tropical waters. It further demonstrates that pristine freshwater environments and marine waters receiving organic loading in the tropics can support densities of C. albicans which may be a health hazard.