RESUMEN

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.

Asunto(s)

Acridinas/química , Inmunoensayo/métodos , Sustancias Luminiscentes/química , Mediciones Luminiscentes/métodos , Animales , Anticuerpos Inmovilizados/química , AMP Cíclico/análisis , Peroxidasa de Rábano Silvestre/análisis , Humanos , Interleucina-8/análisis , Ratones , Antígeno Prostático Específico/análisis , Ratas , Ovinos