RESUMEN

We examined the recently synthesized and characterized polyoxometalate compound (NH4)10[Co2Sb2W20 O70(H2O)6] (POM1). The inhibitory potency of POM1 was studied in tissue culture experiments with uninfected and Rous sarcoma virus (RSV)-infected chicken embryo fibroblasts (CEF) in vivo. We measured a considerable decrease in total cellular phosphotyrosine content in treated infected cells in vivo. POM1 treatment of SR-RSV-A infected CEF in vivo resulted in decreased pp60v-src activity, possibly due to a reduced rate of v-src translation caused by the inhibitory effect of POM1 on the RSV encoded reverse transcriptase activity (RT) activity. In further studies we were able to demonstrate the inhibitory effect of this complex on the RT activity of RSV in vitro and in vivo.

Asunto(s)

Virus del Sarcoma Aviar/fisiología , Transformación Celular Viral/efectos de los fármacos , Fibroblastos/enzimología , ADN Polimerasa Dirigida por ARN/metabolismo , Inhibidores de la Transcriptasa Inversa/farmacología , Compuestos de Tungsteno/farmacología , Animales , Embrión de Pollo , Regulación hacia Abajo , Ensayo de Inmunoadsorción Enzimática , Fibroblastos/virología , Citometría de Flujo , Proteína Oncogénica pp60(v-src)/metabolismo , Fosforilación , Transfección , Proteínas Virales/análisis