RESUMEN

In a search for novel immunostimulating substances we detected that culture supernatants of the gram-positive phytopathogenic bacterium, Rhodococcus fascians, were able to induce cytokine release (TNF(alpha)) from mouse peritoneal macrophages. Monoclonal antibodies were generated against the active principle, and were employed for its isolation and partial characterization as a high molecular (MW>100 kDa) glycoprotein. In addition, methods practicable for its biotechnological preparation and several ELISA variants for its determination were developed.

Asunto(s)

Glicoproteínas/aislamiento & purificación , Factores Inmunológicos/aislamiento & purificación , Rhodococcus/química , Rhodococcus/inmunología , Animales , Anticuerpos Antibacterianos/biosíntesis , Anticuerpos Antibacterianos/química , Anticuerpos Monoclonales/biosíntesis , Anticuerpos Monoclonales/química , Antígenos Bacterianos/química , Antígenos Bacterianos/inmunología , Antígenos Bacterianos/aislamiento & purificación , Línea Celular , Cromatografía de Afinidad , Cromatografía en Gel , Cromatografía por Intercambio Iónico , Femenino , Glicoproteínas/química , Glicoproteínas/inmunología , Factores Inmunológicos/biosíntesis , Factores Inmunológicos/química , Ratones