RESUMEN
Phenolic compounds make up the major secondary metabolites with high pharmaceutical potential. Microalgae were reported to contain low amounts of phenolic compounds. The present study aimed to investigate the hepatoprotective potential of biomass of Spirulina platensis enriched in phenolic compounds. The protective effects of the biomass of S. platensis with low amounts of phenolics (SP1) and with high amounts of phenolics (SP2) against CCl4-induced acute hepatotoxicity were evaluated in rats. The increased levels of ALT, AST and MDA along with decreased activities of SOD and CAT were significantly (p<0.01) ameliorated by SP2. Histological examinations revealed that SP2 was more potent than SP1 in protecting the liver from toxic injury of CCl4 and preserving the hepatocyte ultrastructure. The lesions including necrosis, lymphocyte infiltration, ballooning degeneration and hepatocyte injury as irregular lamellar organisation, dilations in endoplasmic reticulums and the presence of great number of cytoplasmic vacuolization were healed by SP2.
Asunto(s)
Enfermedad Hepática Inducida por Sustancias y Drogas/tratamiento farmacológico , Fenoles/administración & dosificación , Sustancias Protectoras/administración & dosificación , Spirulina/química , Alanina Transaminasa/metabolismo , Animales , Antioxidantes/metabolismo , Aspartato Aminotransferasas/metabolismo , Tetracloruro de Carbono/toxicidad , Enfermedad Hepática Inducida por Sustancias y Drogas/enzimología , Enfermedad Hepática Inducida por Sustancias y Drogas/etiología , Enfermedad Hepática Inducida por Sustancias y Drogas/metabolismo , Glutatión/metabolismo , Glutatión Peroxidasa/metabolismo , Humanos , Hígado/efectos de los fármacos , Hígado/enzimología , Hígado/metabolismo , Masculino , Malondialdehído/metabolismo , Ratas , Ratas Wistar , Superóxido Dismutasa/metabolismoRESUMEN
Effects of Pb accumulation on the contents of chlorophylls (a and b), carotenoid, ascorbic acid (AsA), non-protein SH groups and protein were investigated in aquatic macrophyte Elodea canadensis. Pb accumulation in E. canadensis tissues increased with increasing metal concentrations. The increases at 1, 10 and 100 mg/L Pb are about 12.0, 44.6 and 71.1 times greater than control, respectively. Contents of chlorophylls, carotenoid and protein were adversely affected by Pb accumulation. Induction of non-protein SH groups and AsA showed that Pb accumulation caused oxidative stress. It is also possible that increased non-protein SH groups by Pb accumulation may be due to their role in Pb detoxification.
Asunto(s)
Hydrocharitaceae/efectos de los fármacos , Plomo/toxicidad , Contaminantes Químicos del Agua/toxicidad , Ácido Ascórbico/metabolismo , Carotenoides/metabolismo , Clorofila/metabolismo , Clorofila A , Hydrocharitaceae/metabolismo , Plomo/metabolismo , Estrés Oxidativo/efectos de los fármacos , Proteínas de Plantas/metabolismo , Contaminantes Químicos del Agua/metabolismoRESUMEN
In this study, effect of different 2,4 -dichlorophenoxyacetic acid (2,4-D) concentrations (0.0, 9.10(-5), 9.10(-4), 9.10(-3) and 9.10(-2) mM) on growth rate, content of protein and chlorophyll-a in Chlorella vulgaris and Spirulina platensis cells was investigated. The most stimulatory effect on growth rate, protein and pigment ratio of C. vulgaris and S. platensis was observed at 9.10(-4) mM concentrations of 2,4-D. The results show that low concentrations of 2,4-D have hormonal effect due to being a synthetic auxin. Cell number protein and pigment rates were inhibited at 9.10(-2) mM concentration in C. vulgaris. Such parameters were inhibited in S. platensis, both at 9.10(-3) and 9.10(-2) mM 2,4-D concentrations. This is due to herbicidal effect of high concentrations of 2,4-D. S. platensis was found to be more sensitive than S. vulgaris to 2,4-D applications. The use of algae as bio-indicators in herbicide contaminated fresh water habitats, was discussed.