RESUMEN
For the purpose of new detritylation agents search for microarray oligonucleotide synthesis we investigated applicability of [4-(4-methoxyphenyl)-2,6-dinitro-phenyl](phenyl)methyl 2,2,2-trichloroacetate for 4,4'-dimethoxytrityl group detritylation during oligonucleotide syntheses generating trichloroacetic acid at radiation by light with a length of wave of 405 nanometers. [4-(4-Methoxyphenyl)-2,6-dinitro-phenyl](phenyl)methyl 2,2,2-trichloroacetate has been successfully used for solid-phase oligonucleotide synthesis of desired oligonucleotides.
Asunto(s)
Oligonucleótidos/síntesis química , Ácido Tricloroacético/síntesis química , Cinética , Luz , Procedimientos Analíticos en Microchip , Estructura Molecular , Oligonucleótidos/química , Ácido Tricloroacético/químicaRESUMEN
The paper presents the analysis of present-day approaches to solution of the problem of spa-treatment method choice. Principal mathematical means and methodological approaches consist of; formalization of the subject field; accumulation of sufficient clinical material; classification of the patients' condition, functional changes and methods of treatment, multivariate (cluster, factor), inductive formation of concepts, models of thinking, etc.
Asunto(s)
Toma de Decisiones Asistida por Computador , Colonias de Salud , Enfermedades Pulmonares Obstructivas/rehabilitación , Modalidades de Fisioterapia/métodos , Técnicas de Apoyo para la Decisión , Sistemas Especialistas , Humanos , Análisis de RegresiónRESUMEN
The changes in the system of hemostasis were studied in 36 patients with sepsis managed with sorptive detoxication. The starting status of the system of hemostasis in patients with favorable outcome of the disease can be considered as the 2d stage of the disseminated intravascular coagulation (DIC), and in patients with the unfavorable outcome--as the 3d (hypocoagulative) stage. The sorptive detoxication provides the treatment of the DIC syndrome in favorable outcome, as well as further progression of the process in unfavorable outcome of sepsis. Conservative treatment provides stabilization of the hemostatic system and gives opportunity to perform delayed hemosorption with a positive clinical result.
Asunto(s)
Hemoperfusión , Hemostasis/fisiología , Sepsis/sangre , Adolescente , Adulto , Anciano , Coagulación Intravascular Diseminada/sangre , Coagulación Intravascular Diseminada/etiología , Coagulación Intravascular Diseminada/terapia , Femenino , Humanos , Masculino , Persona de Mediana Edad , Sepsis/complicaciones , Sepsis/terapia , Infección de la Herida Quirúrgica/sangre , Infección de la Herida Quirúrgica/complicaciones , Resultado del TratamientoRESUMEN
The experiments on rats with overload heart failure have shown that creatine phosphate (CP) used in a single dose at the peak of severe signs of cardiac decompensation was found to produce a short-term normalization of rhythm and an increase in heart rate. With multiple doses, the agent slowed down the development of bradycardia, prevented arrhythmias and promoted the rise of longevity in the experimental animals. At the same time, exogenous CP failed to affect energy-dependent processes of re- and depolarization which were impaired in the myocardium in acute heart failure.
Asunto(s)
Antiarrítmicos/uso terapéutico , Insuficiencia Cardíaca/tratamiento farmacológico , Corazón/efectos de los fármacos , Fosfocreatina/uso terapéutico , Enfermedad Aguda , Animales , Evaluación Preclínica de Medicamentos , Electrocardiografía/efectos de los fármacos , Electrofisiología , Metabolismo Energético/efectos de los fármacos , Corazón/fisiopatología , Insuficiencia Cardíaca/fisiopatología , Miocardio/metabolismo , RatasRESUMEN
Experiments with rats undergoing overload testing showed that exogenous creatine phosphate was likely to fail to penetrate into myocardiocytes and to affect their energy balance directly. However, during its long-term administration in energy deficiency, creatine phosphate was able to maintain cardiac pump function via activation of processes controlling sinus nodal automatism and atrioventricular conduction.