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The stability, sensitivity, and volatility of essential oils are some of their most serious limitations, and nanoencapsulation has been considered one of the most effective techniques for solving these problems. This research aimed to investigate the incorporation of Salvia rosmarinus Speen and Cedrus atlantica Manetti (MEO) essential oil mixture in Gum Arabic (GA) and to evaluate nanoencapsulation's ability to promote antifungal activity against two brown rot fungi responsible for wood decay Gloeophyllum trabeum and Poria placenta. The optimization of encapsulation efficiency was performed using response surface methodology (RSM) with two parameters: solid-to-solid (MEO/GA ratio) and solid-to-liquid (MEO/ethanol). The recovered powder characterization was followed by various techniques using a scanning electron microscope (SEM), X-ray diffractometry (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR), and thermo-gravimetric analysis (TGA). The optimal nanoencapsulating conditions obtained from RSM were ratios of MEO/GA of 1:10 (w/w) and MEO/ethanol of 10% (v/v), which provided the greatest encapsulation efficiency (87%). The results of SEM, XRD, DLS, FTIR, and TGA showed that the encapsulation of MEO using GA modified particle form and molecular structure and increased thermal stability. An antifungal activity assay indicated that an effective concentration of MEO had an inhibitory effect on brown rot fungi. It had 50% of the maximal effect (EC50) value of 5.15 ± 0.88 µg/mL and 12.63 ± 0.65 µg/mL for G. trabeum and P. placenta, respectively. Therefore, this product has a great potential as a natural wood preservative for sustainable construction and green building.

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Chemical fungicides are often harmful to people and the environment because of their toxicity. The wood protection industry places a high priority on replacing them with natural products. Therefore, this investigation focused on developing a formulation of a binary combination of Salvia rosmarinus Spenn and Cedrus atlantica Manetti obtained by Simultaneous hydrodistillation to protect the wood from decay using a mixture design methodology. The chemical composition of essential oil was identified by gas chromatography coupled with mass spectrometry (GC/MS), and their anti-wood-decay fungal activity was assessed using the macrodilution method against four fungi responsible for wood decay: Coniophora puteana, Coriolus versicolor, Gloeophyllum trabeum, and Poria placenta. The results of GC/MS identified myrtenal as a new component appearing in all binary combinations. The optimum anti-wood-decay fungal activity was observed in a combination of 60% S. rosmarinus and 40% C. atlantica essential oils, providing an effective concentration for 50% of maximal effect (EC50) value of 9.91 ± 1.91 and 9.28 ± 1.55 µg/mL for C. puteana and C. versicolor, respectively. The highest anti-wood-decay fungal activity for G. trabeum and P. placenta was found in the combination of 55% of S. rosmarinus and 45% of C. atlantica essential oils, with EC50 values of 11.48 ± 3.73 and 22.619 ± 3.79 µg/mL, respectively. Combined simultaneous hydrodistillation improved the antifungal effect of these essential oils. These results could be used to improve antifungal activity and protect wood against wood-decay fungi.

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ETHNOPHARMACOLOGICAL RELEVANCE: Throughout history, Humans have always used tar for different purposes such as gluing materials, waterproofing, and conserving wood, but today, it is also used in medicines and cosmetics. Many countries around the world, Norway and Morocco to name a few, obtain tar from endemic trees. In a process of dry distillation, the organic material, endemic trees in this case, is exposed to a high temperature with a restricted amount of oxygen. Eventually, it cracks the large molecules in the matter and turns it into tar. AIM OF THE REVIEW: This review article sheds light on tar production and the species that have been used in the process. Equal emphasis is placed on its uses, chemical composition, and toxicity. MATERIALS AND METHODS: Meta-analysis (PRISMA) guidelines have been used to review this article. The review is put together from various articles, theses, documents in "Science Direct", "Hal (Archive ouvert)", "Web of knowledge" without limitation date. RESULTS: It turns out that tar can be produced by 18 tree taxonomic families, notably Cupressaceae, Pinaceae, and Betulaceae. As for the production techniques, two methods are considered: Per ascensum and Per descensum, which can take different forms. The chemical composition of tar consists of acids, phenols, and aromatic hydrocarbons. The uses of tar have changed over time, while its toxicity is strongly related to its chemical composition. CONCLUSIONS: The different species used in tar production have been highlighted in this research review. Equal importance has been given to its methods of extraction, uses and its chemical components. We hope that future studies will focus more on these species used to produce tar in other biological activities.

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The main odorant constituents of Cedrus atlantica essential oil were characterized by GC-Olfactometry (GC-O), using the Aroma Extract Dilution Analysis (AEDA) methodology with 12 panelists. The two most potent odor-active constituents were vestitenone and 4-acetyl-1-methylcyclohexene. The identification of the odorants was realized by a detailed fractionation of the essential oil by liquid-liquid basic extraction, distillation and column chromatography, followed by the GC-MS and GC-O analyses of some fractions, and the synthesis of some non-commercial reference constituents.

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Deodarone [2,2,6-trimethyl-6-(4-methylcyclohex-3-enyl)-tetrahydro-4-pyrone] is a sesquiterpene tetrahydro-γ-pyrone related to bisabolene and atlantone, first isolated from Cedrus deodora essential oil. With respect to the stereochemistry of the asymmetric carbons C4 and C8, two diastereoisomers may be distinguished. Identification and quantification of both diastereoisomers in wood and tar oils from C. atlantica has been achieved using 13C NMR spectroscopy, in combination with GC (polar column). The contents of (4R,8R)- and (4R,8S)-deodarone varied between 1.1-2.8% and 1.0-3.0%, respectively.

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