RESUMEN
We have developed a novel method for nanometer-sized droplet deposition of electrically conductive liquids based on the dynamic mode atomic force microscopy (AFM). We succeeded in depositing liquid droplets with a volume of ten zeptoliters or smaller and in making arrays of the droplets with a spacing of several tens of nanometers using the AFM cantilever with a small aperture fabricated by a focused ion beam. The liquid droplets were deposited through the aperture by applying an electric field between the liquid and a conductive surface.
RESUMEN
A 62-year-old man with an ventricular pacemaker for sick sinus syndrome received right shoulder arthroplasty. During the intraoperative discrepancy between heart rate and pulse rate, continuous infusion of low dose landiolol was useful to control his heart rate of atrial fibrillation.
Asunto(s)
Antiasmáticos/administración & dosificación , Fibrilación Atrial/tratamiento farmacológico , Fibrilación Atrial/etiología , Estimulación Cardíaca Artificial/efectos adversos , Frecuencia Cardíaca , Cuidados Intraoperatorios , Complicaciones Intraoperatorias/tratamiento farmacológico , Morfolinas/administración & dosificación , Síndrome del Seno Enfermo/complicaciones , Urea/análogos & derivados , Anestesia General , Artroplastia de Reemplazo , Fibrilación Atrial/fisiopatología , Ventrículos Cardíacos , Humanos , Infusiones Intravenosas , Complicaciones Intraoperatorias/etiología , Masculino , Persona de Mediana Edad , Articulación del Hombro/cirugía , Resultado del Tratamiento , Urea/administración & dosificaciónRESUMEN
When single embryoid bodies of teratocarcinoma OTT 6050 were cultured by the diffusion chamber technique in the peritoneal cavity of a mouse, they lost their characteristic three-dimensional structure early in the culture period and proliferated logarithmically up to the 60th day of culture with a doubling time of 3.7 days, forming cell layers that adhered to the surface of the membrane filters of the diffusion chamber. They continued further to proliferate at a lower rate up to the 80th day of culture. At the 60th day, many round cells, classified by diameter into about three classes, were observed on the membrane filters. The tumorigenicity of these cells derived from the chamber cultures was much less than that of embryoid bodies injected directly into the abdominal cavity, judging from the number of days the mice survived.