RESUMEN
The raising burden of cancer can be controlled by fabricating smart nanomaterials that can detect tumours easily. In this study, we report about the preparation of radiopaque fibrin nanocomplex (RFN) for imaging solid tumours. The nanocomplex exhibits high X-ray absorption and therefore utilizes X-ray radiography and computed tomography (CT) for imaging tumours. The CT images taken after intratumoral administration of RFN in tumor bearing mice displayed excellent visibility of tumour. Moreover, increased amount of RFN was seen at the site of tumour after 45 min of post-injection. These research findings prove the promising use of RFN as a valuable tool for imaging solid tumours.
Asunto(s)
Tomografía Computarizada por Rayos XRESUMEN
BACKGROUND: A variety of bioimaging tools assists in the diagnosis and evaluation of rheumatoid arthritis (RA) and other osteoarthritis. However, detection of RA in the early stages by targeting its macrophages with suitable contrast agents will help in arresting the progression of the disease. METHODS: In the present study, we investigated the effectiveness of using magnetic fibrin nanoparticles (MFNPs) conjugated with folic acid (FA-MFNPs) as a specific contrast agent to target the activated macrophages, which overexpress the folate receptors (FR) in the knee joints of rats with antigen-induced arthritis (AIA). RESULTS: FA-MFNPs were spherical with an average size of 18.3±1.6nm. In vitro studies have shown effective internalization of FA-MFNPs into the Raw264.7 macrophage cells. In vivo studies were carried out by injecting FA-MFNPs intravenously into the arthritic rats. The results showed enhanced MR imaging in the synovium of arthritic joints. Prussian blue histological staining confirmed uptake of FA-MFNPs by macrophages in the synovial tissue. CONCLUSION: The animal experiment results indicate that FA-MFNPs can be used as a specific MRI contrast agent in identifying phagocytic active macrophages in the synovial joints. GENERAL SIGNIFICANCE: Blood is the precursor source for synthesising the fibrin-based iron oxide (magnetic) nanoparticles (MFNPs) with diameters between 12 and 15nm. It has excellent superparamagnetic behaviour, biocompatibility, osteogenic potency, hemocompatibility, and biodegradable properties. MFNPs-based nanocomposites might be a promising contrast agent for bioimaging.
Asunto(s)
Artritis Reumatoide/diagnóstico , Artritis Reumatoide/patología , Medios de Contraste/química , Fibrina/metabolismo , Macrófagos/metabolismo , Imagen por Resonancia Magnética/métodos , Nanopartículas de Magnetita/química , Animales , Endocitosis/efectos de los fármacos , Ácido Fólico/metabolismo , Cabras , Hidrodinámica , Hígado/efectos de los fármacos , Hígado/patología , Macrófagos/efectos de los fármacos , Nanopartículas de Magnetita/ultraestructura , Masculino , Ratones , Polvos , Células RAW 264.7 , Ratas Wistar , Espectrofotometría Ultravioleta , Espectroscopía Infrarroja por Transformada de Fourier , Coloración y Etiquetado , Electricidad Estática , Difracción de Rayos XRESUMEN
PURPOSE: Bluefin Trevally (Caranx melampygus) fish is mainly used for fillet production, the bones of which are discarded as a major solid waste in the fish food processing industry. In the present study, novel collagen films were prepared using the bones of Bluefin Trevally (BT). The study investigates the potential of using this collagen film as a wound dressing material. METHODS: The prepared collagen films (CFs) were characterized for their physicochemical properties using Fourier transform infrared spectroscopy (FTIR), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), atomic force microscopy (AFM), tensile strength, elongation at break, etc. In vitro studies using human keratinocyte cell line (HaCaT) also proved the biocompatibility of CF. The CFs were used as wound dressing material on the experimental wounds of rats and the healing pattern was evaluated using planimetric and histopathological studies. RESULTS: CF prepared from the bones of BT possessed better mechanical properties. The in vitro studies demonstrated its biocompatible nature. Acceleration of wound healing in CF-treated rats was evident in the in vivo studies. CONCLUSIONS: The study has devised a process for using fish waste in the preparation of a value-added product like wound dressing material. The CF with the required strength, biocompatibility and wound healing properties may be tried as a wound dressing material in large animals after obtaining the necessary approval.
Asunto(s)
Vendajes , Huesos/química , Colágeno/análisis , Peces , Residuos Industriales , Animales , Materiales Biocompatibles , Línea Celular , Supervivencia Celular , Industria de Procesamiento de Alimentos , Humanos , Queratinocitos/fisiología , Ratas Wistar , Cicatrización de HeridasRESUMEN
There is an emerging need for the development of new anticancer nanocomposite which exhibits imaging properties and targeted drug delivery. In the present study, a nanobiocomposite was prepared, in this direction, which contains carbon nanoparticles (CNP), methotrexate (Mtx) and asparaginase (Asp) coupled with fluorescein isothiocyanate (FITC). The prepared nanobiocomposite kills only the cancer cells due to the presence of Mtx which is a folic acid analogue and targets the cancer cells due to the over expression of folate receptors on their surface and apoptosis occurs due to the anticancer activity of enzyme asparaginase. The results obtained from the present study confirmed the sustained release of Mtx and Asp from the nanobiocomposite. The nanobiocomposite was found to be haemocompatible, biocompatible and showed more than 90% apoptosis. The drug pathway was clearly monitored due to the presence of FITC in the nanobiocomposite. This study proves the effectiveness of the fabricated nanoconjugate, as it helps in imaging, targeting and destructing the cancerous cells. The prepared nanoconjugate may be effectively applied in in vivo experiments before applying on to humans.
Asunto(s)
Antineoplásicos/administración & dosificación , Asparaginasa/administración & dosificación , Carbono/química , Portadores de Fármacos/química , Transportadores de Ácido Fólico/metabolismo , Metotrexato/administración & dosificación , Nanopartículas/química , Células 3T3 , Animales , Antineoplásicos/farmacología , Apoptosis/efectos de los fármacos , Asparaginasa/farmacología , Línea Celular Tumoral , Supervivencia Celular/efectos de los fármacos , Liberación de Fármacos , Hemólisis/efectos de los fármacos , Humanos , Metotrexato/farmacología , Ratones , Microscopía de Fuerza Atómica , Nanocompuestos/administración & dosificación , Nanocompuestos/química , Espectroscopía Infrarroja por Transformada de Fourier , Propiedades de Superficie/efectos de los fármacosRESUMEN
The effect of Gallic acid (GA) in the presence of 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-hydroxysuccinimide (NHS) on collagen scaffold is investigated. The thermal mechanical analyzer (TMA), differential scanning calorimetric (DSC), and thermogravimetric analysis (TGA) including tensile strength (TS, 180 ± 3 MPa), denaturation temperature (Td, 80.03°C), % elongation (% E, 180 ± 9) and weight loss (31.76%), indicate that the modification improves the structural integrity and stability of the collagen scaffold. The GA-EDC/NHS treatment inhibits the action of collagenase against collagen degradation compared to GA and EDC/NHS. It is concluded from docking studies that GA binds with collagen like peptide (CLP) and collagenase through multiple H-bonds and hydrophobic interactions leading to low binding energy -5.1 and -5.3 Kcal/mol, respectively. The hydrophobic core of the GA molecules, probably incorporates itself into the hydrophobic areas of the collagen groups, whereas OH and COOH moieties of GA establish multiple H-bonds with neighboring collagen molecules and carboxamide bond, thereby improving the swelling and water uptake properties, biocompatibility and cell adhesion properties. This results in improving stability of the scaffold, which prevents the free access of the collagenase to reactive sites in the triple helical collagen chains.
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Colágeno/química , Ácido Gálico/química , Andamios del Tejido , Animales , Rastreo Diferencial de Calorimetría , Bovinos , Clostridium histolyticum/enzimología , Colagenasas/metabolismo , Interacciones Hidrofóbicas e Hidrofílicas , Ratones , Células 3T3 NIH , Espectroscopía Infrarroja por Transformada de Fourier , TermogravimetríaRESUMEN
Open burn wounds require proper dressings for faster healing and to prevent infection. In the present study, a wound dressing material in sheet form, containing fish scale collagen (FSC), physiologically clotted fibrin (PCF) and Macrotyloma uniflorum plant extract (MPE) was applied on the experimental wounds of rats. It was found that MPE accelerated wound healing, by suppressing the cyclooxygenase-2 (COX-2) and inducible nitric oxide synthases (iNOS) expressions thereby reduced inflammation. It has influenced the regulation of growth factors such as vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), epidermal growth factor (EGF) and transforming growth factor (TGF-ß). The biocomposite sheet has enhanced collagen synthesis and down regulated the matrix metalloproteinases (MMPs), thereby helped faster healing of wounds. The results suggest that the incorporation of MPE played an important role in enhancing the wound healing rate. The FSC-PCF biosheet containing MPE may be further tried on the clinical wounds of small and large animals before application on to humans.
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Vendajes , Colágeno/farmacología , Citocinas/farmacología , Fabaceae/química , Mediadores de Inflamación/farmacología , Péptidos y Proteínas de Señalización Intercelular/farmacología , Extractos Vegetales/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Ciclooxigenasa 2/metabolismo , Epitelio/efectos de los fármacos , Femenino , Peces , Perfilación de la Expresión Génica , Hexosaminas/metabolismo , Hidroxiprolina/metabolismo , Inmunohistoquímica , Péptidos y Proteínas de Señalización Intercelular/genética , Péptidos y Proteínas de Señalización Intercelular/metabolismo , Masculino , Óxido Nítrico Sintasa de Tipo II/metabolismo , ARN Mensajero/genética , ARN Mensajero/metabolismo , Ratas Wistar , Resistencia a la Tracción/efectos de los fármacos , Ácidos Urónicos/metabolismoRESUMEN
Click chemistry approaches are tailored to generate molecular building blocks quickly and reliably by joining small units together selectively and covalently, stably and irreversibly. The vegetable tannins such as hydrolyzable and condensed tannins are capable to produce rather stable radicals or inhibit the progress of radicals and are prone to oxidations such as photo and auto-oxidation, and their anti-oxidant nature is well known. A lot remains to be done to understand the extent of the variation of leather stability, color variation (lightening and darkening reaction of leather), and poor resistance to water uptake for prolonged periods. In the present study, we have reported click chemistry approaches to accelerated vegetable tanning processes based on periodates catalyzed formation of oxidized hydrolysable and condensed tannins for high exhaustion with improved properties. The distribution of oxidized vegetable tannin, the thermal stability such as shrinkage temperature (T s) and denaturation temperature (T d), resistance to collagenolytic activities, and organoleptic properties of tanned leather as well as the evaluations of eco-friendly characteristics were investigated. Scanning electron microscopic analysis indicates the cross section of tightness of the leather. Differential scanning calorimetric analysis shows that the T d of leather is more than that of vegetable tanned or equal to aldehyde tanned one. The leathers exhibited fullness, softness, good color, and general appearance when compared to non-oxidized vegetable tannin. The developed process benefits from significant reduction in total solids and better biodegradability in the effluent, compared to non-oxidized vegetable tannins.
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Química Clic/métodos , Extractos Vegetales/química , Taninos/química , Verduras/química , Oxidación-Reducción , TemperaturaRESUMEN
The modification of carbon nanoparticles (CNPs) using biological molecules is important in the field of chemical biology, as the CNPs have the potential to deliver the drugs directly to the targeted cells and tissues. We have modified the CNPs by coating bovine serum albumin (BSA) on their surfaces and loaded with methotrexate (Mtx). Infrared spectra have revealed the coating of BSA and Mtx on CNP (CBM). Scanning electron microscopy (SEM) and atomic force microscope (AFM) pictures have exhibited the spherical nature of the composite and coating of the proteins on CNPs. The prepared CBM biocomposite has exhibited a sustained release of drug. MTT assay using A549 lung cancer cell lines has revealed 83% cell death at 150 µg/ml concentration of CBM. These results indicate that CNPs based biocomposites may be tried as therapeutic agents in treatment of cancer like diseases.
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Antineoplásicos/farmacología , Carbono/metabolismo , Portadores de Fármacos/química , Sistemas de Liberación de Medicamentos , Metotrexato/farmacología , Nanopartículas/química , Albúmina Sérica Bovina/metabolismo , Animales , Bovinos , Muerte Celular/efectos de los fármacos , Línea Celular Tumoral , Hemólisis/efectos de los fármacos , Humanos , Microscopía de Fuerza Atómica , Nanopartículas/toxicidad , Nanopartículas/ultraestructura , Espectroscopía Infrarroja por Transformada de Fourier , Espectrometría Raman , Termogravimetría , Difracción de Rayos XRESUMEN
Application of plant extracts for the burn/wound treatment is followed over the decades as a common practice and it is an important aspect in clinical management. In this study porous collagen sponges (CS) were prepared using fish scales and were incorporated with mupirocin (CSM) and extracts of Macrotyloma uniflorum (CSPE) separately to impart antimicrobial activity to the sponges. The results showed that the addition of plant extract increased the tensile strength of CSPE and stability against collagenase enzyme. FTIR studies have shown the incorporation of plant extract in CSPE, SEM studies have revealed the porous nature of the sponges and XRD patterns have shown the retention of collagen triple helical structure even after the addition of plant extract. CSPE and CSM have exhibited antimicrobial properties. The sponges prepared were analysed for their in vitro biocompatibility studies using fibroblasts and keratinocyte cell lines and the results have shown their biocompatible nature. Based on the results obtained, CS, CSM and CSPE may be tried as a burn/wound dressing materials, initially, in small animals in vivo.
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Quemaduras , Colágeno/química , Fabaceae/química , Extractos Vegetales/farmacología , Cicatrización de Heridas/efectos de los fármacos , Animales , Antiinfecciosos/química , Antiinfecciosos/farmacología , Bacillus subtilis/efectos de los fármacos , Materiales Biocompatibles/química , Supervivencia Celular/efectos de los fármacos , Colagenasas/metabolismo , Peces , Ratones , Mupirocina/química , Células 3T3 NIH , Extractos Vegetales/química , Porosidad , Espectroscopía Infrarroja por Transformada de Fourier , Staphylococcus aureus/efectos de los fármacos , Resistencia a la Tracción , Difracción de Rayos XRESUMEN
Developing biomaterials having wound healing properties within the search of a common man is the need of hour, particularly in developing and third world countries. Keeping this objective in view we have developed a wound dressing material, in sheet form, containing fish scale collagen (FSC) and physiologically clotted fibrin (PCF), both are by products of aqua food and meat industries respectively. To impart antimicrobial properties to the composite sheet, it was incorporated with Macrotyloma uniflorum plant extract (MPE). SEM pictures have shown that FSC:PCF:MPE composite has fibrous and porous surface which helps in transportation of oxygen as well as absorbing wound fluids and their evaporation. The biomaterials have shown 100% biocompatibility and the percentage cell viability was found to be above 89%. The FSC:PCF:MPE biocomposite film with required mechanical strength, biocompatibility and antimicrobial properties can be tried as a burn/wound dressing material.
Asunto(s)
Vendajes , Materiales Biocompatibles/química , Animales , Colágeno/química , Fibrina/química , Peces/metabolismo , Piel/químicaRESUMEN
The ex vivo cultured limbal stem cells over a biocompatible scaffold are used in the management of limbal stem cell deficiency as an ideal replacement for human amniotic membrane (HAM). A novel source of collagen from fish scales (FSC) was used to fabricate the scaffold. In this study, we have evaluated the physicochemical, mechanical, and culture characteristics of FSC and compared with denuded HAM. The cultured corneal cells were characterized by real-time polymerase chain reaction for putative stem cell markers. The swelling ratio, collagenase assay, and microbial resistance of FSC gave better results when compared to those of HAM. The mechanical and physical strengths of FSC were good enough to handle when compared to HAM. Under microscopic observation, epithelial migration was noted at the end of 48 h from limbal explants plated on FSC and on HAM at the end of 72 h. By the end of the 15th day, 90 to 100% confluent growth was seen resembling the morphological features of limbal epithelium. In conclusion, FSCs from a novel renewable biological source were optically clear with sufficient strength, and gave encouraging results in culture studies; the same may be tried as potential candidate for corneal transplantation after in vivo studies.
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Colágeno/química , Córnea/citología , Epitelio Corneal/citología , Peces , Ingeniería de Tejidos/métodos , Andamios del Tejido/química , Amnios/citología , Animales , Proliferación Celular , Células Cultivadas , Colágeno/aislamiento & purificación , Colágeno/metabolismo , Colagenasas/metabolismo , Córnea/metabolismo , Epitelio Corneal/metabolismo , Peces/anatomía & histología , Peces/metabolismo , Regulación de la Expresión Génica , Humanos , Limbo de la Córnea/citología , Limbo de la Córnea/metabolismo , Resistencia a la Tracción , Andamios del Tejido/microbiologíaRESUMEN
AIM: The aim of this study was to compare some of the physicochemical characteristics of type 2 diabetic bones (DBs) and normal bones (NBs). MATERIALS AND METHODS: The organic and inorganic parts of human NBs and DBs were separated using conventional methods, and their physicochemical characteristics were compared using infrared (IR) spectroscopy, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, thermogravimetric analysis, X-ray diffraction (XRD), and scanning electron microscopy (SEM). RESULTS: The IR spectrum of the collagen part of DBs has showed the presence of carbonyl groups, indicating cross-linking in the α-chain. This was also confirmed by the increased thermal stability (22°C) in DB collagen. The XRD data of the inorganic part of DBs have revealed decreased crystallinity. SEM images of the inorganic part of DBs have shown a porous (weak) nature compared to those of NBs, which exhibited a compact (healthy) nature. CONCLUSION: The cross-linking in DB collagen molecules and the decreased and defective crystallinity in the inorganic portion of DBs might be the reasons for the increased risk of fracture among diabetic patients.
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Enfermedades Óseas/diagnóstico , Enfermedades Óseas/fisiopatología , Huesos/química , Huesos/fisiología , Diabetes Mellitus Tipo 2/fisiopatología , Absorciometría de Fotón , Densidad Ósea , Huesos/ultraestructura , Electroforesis en Gel de Poliacrilamida , Humanos , Microscopía Electrónica de Rastreo , Espectrofotometría Infrarroja , TermogravimetríaRESUMEN
In this report, a novel method for the synthesis of silver nanoparticles on the surface of hydroxyapatite is described. Hydroxyapatite crystals are synthesized from acid mineralized solution, a byproduct of bone glue industries, by a simple chemical precipitation method, which results in the formation of hydroxyapatite nanocrystals. The reduction of silver ions occurs by the electron transfer from the hydroxyl groups on the surface of hydroxyapatite. This results in the formation of silver nanorods and needle shaped nanoparticles that are bound on the surface of hydroxyapatite, and the observed silver nanocrystals show anisotropic structure. Thus, hydroxyapatite crystals can be used as a new class of inorganic scaffolds for the synthesis of nanomaterials with implications in designing inorganic-inorganic hybrid nanocomposites for different applications.